Acute Dermal Toxicity Study of Areca Catechu Linn. Extract in Sprague-Dawley Rats

Abstract

Objective: Areca catechu Linn. or biji pinang is one of the most widely used psychoactive substance with several hundred million users worldwide, predominantly in Southern Asia. However, details of the dermal toxicity of A. catechu L. are still undiscovered. The objective of this study is to investigate the in vivo acute dermal toxicity of aqueous extract of A. catechu L. at dose 15,000 mg/kg body weight in Sprague-Dawley rats. Methods:The acute dermal toxicity of A. catechu L. nut extract was investigated in rats, as per OECD Guidelines 402 for acute toxicity protocols. The body weight, possibility of death, general signs, and behavior activity parameters were measured for 14 days to ascertain the median lethal dose (LD50) of the extract. At the end of the study, all the animals in all the treated group were sacrificed. Results:The LD50 was found to be >15,000 mg/kg body weight. There was significant weight increase (p<0.05) in treated group when compared to control group. No mortality was observed during whole 14 days study period. A single dose of 15,000 mg/kg of body weight did not produce treatment-related signs of toxicity in any of animal tested. Conclusion:A single dermal dose to A. catechu L. aqueous extract had no toxic effects on mortality, clinical signs, body weight changes, and gross findings in female rats at a dose of 15,000 mg/kg of body weight. Subsequently, the concentrate can be employed for pharmaceuticals nutrient plants.

Investigation of anti-Alzheimer’s activity of aqueous extract of areca nuts (Areca catechu L.): In vitro and in vivo studies

“…Our results from MWM test revealed that the extract at the doses of 1.5 and 3 mg/kg could reverse scopolamine-induced memory deficit in rats. Considering the fact that LD50 of the aqueous extract of betel nuts was found to be ≥15.000 mg/kg body weight (Sari et al, 2014;Kim et al, 2018), using very low toxic dose of extract would be beneficial to afford an herbal remedy possessing multi-target activity for the treatment of AD.…”

Acute Oral Toxicity Study of Ethanolic Extract of Actinoscirpus Grossus (L.F.) Goetgh. And D.A. Simpson

“…Acute oral toxicity is usually carried out in small groups of animal's gives information about the toxic profile of the plant extract. In any pharmaceutical preparation, acute oral toxicity studies are necessary to use in humans [14][15][16]. In the present study, the acute oral toxicity of ethanolic extract of A. grossus (L.f.) Goetgh.…”

MANFAAT DAN TOKSISITAS PINANG (Areca catechu) DALAM KESEHATAN MANUSIA

“…Batang lurus, tidak bercabang dan kokoh mengakibatkan AC digunakan sebagai pembatas lahan maupun pembatas pekarangan (Silalahi 2014). Bagi masyarakat lokal Indonesia, AC juga digunakan sebagai tanaman pekarangan (Sari et al 2014) dan juga pembatas ladang (Silalahi et al 2014). Silalahi et al (2015b) menyatakan bahwa buah AC telah lama diperjual-belikan oleh pedagang tumbuhan obat di pasar Kabanjahe sebagai obat tradisonal, bahan ritual maupun tujuan lainnya.…”

“…Bahan untuk menyirih pinang (AC), perbungaan atau daun Piper betle, dan kapur sirih mengakibatkan kanker oral di Asia Tenggara (Chiba et al 2001). Sari et al (2014) menyatakan bahwa di berbagai daerah Indonesia AC digunakan sebagai obat untuk mengatasi pendarahan seperti dalam menstruation, epistaxis ulcer, difteri, infeksi parasit, diare, dan disentri.…”