PurposeThe current study aimed to examine the acute effect of physioball wall squat training on knee joint active reposition senses and isokinetic parameters of knee flexion and extension in sedentary collegiate female students.MethodsAn experimental study was conducted with sixty sedentary female college students aged between 18 to 25 who were divided into two groups. The experimental group of 30 participants performed Swiss ball wall squats, spanning three sets of 12 repetitions each, with 30-second rest intervals between sets. The control group of 30 participants stood with a Swiss ball positioned behind their backs, replicating the same parameters as the experimental group but without squatting. The isokinetic 4 Pro device measured active knee joint repositioning senses and isokinetic parameters of knee flexion and extension.ResultsThe findings revealed notable improvements in active knee repositioning senses and all isokinetic parameters of knee flexion and extension (<i>p</i> < 0.05) in the experimental group post-training when compared to the control group. There were also significant differences detected between pre- and post-Swiss ball training intervention in the experimental group for all variables (<i>p</i> < 0.05).ConclusionsThese results suggest that Swiss ball wall squat training could enhance knee joint position senses and isokinetic parameters, potentially reducing knee injuries in sedentary individuals.