Acute effects of resistance exercise and intermittent intense aerobic exercise on blood cell count and oxidative stress in trained middle-aged women

Abstract

The aim of this study was to compare the effect of an intermittent intense aerobic exercise session and a resistance exercise session on blood cell counts and oxidative stress parameters in middle-aged women. Thirty-four women were selected and divided into three groups: RE group (performing 60 min of resistance exercises, N = 12), spinning group (performing 60 min of spinning, N = 12), and control group (not exercising regularly, N = 10). In both exercise groups, lymphocytes and monocytes decreased after 1-h recuperation (post-exercise) compared to immediately after exercise (P < 0.05). Immediately after exercise, in both exercised groups, a significant increase in TBARS (from 16.5 ± 2 to 25 ± 2 for the spinning group and from 18.6 ± 1 to 28.2 ± 3 nmol MDA/mL serum for the RE group) and protein carbonyl (from 1.0 ± 0.3 to 1.6 ± 0.2 for the spinning group and from 0.9 ± 0.2 to 1.5 ± 0.2 nmol/mg protein for the RE group) was observed (P < 0.05). A decrease in antioxidant activities (non-protein sulfhydryl, superoxide dismutase, catalase) was also demonstrated with a negative correlation between damage markers and antioxidant body defenses (P < 0.05). These results indicate that an acute bout of intermittent or anaerobic exercise induces immune suppression and increases the production of reactive oxygen species, causing oxidative stress in middle-aged and trained women. Furthermore, we demonstrated that trained women show improved antioxidant capacity and lower oxidative damage than sedentary ones, demonstrating the benefits of chronic regular physical activity.

A session of resistance exercise increases vasodilation in intermittent claudication patients

“…In regards to oxidative stress, resistance exercise did not lead to a change in MDA in PAD patients, which differs from the results obtained in previous studies in healthy subjects (Cardoso et al 2012;Rahimi 2011). The differences in results may be attributed to exercise intensity, since this study employed low-intensity resistance exercise (ϳ55% of 1-repetition maximum (1RM)), while previous studies used greater intensities (ϳ70% of 1RM).…”

Effects of exercise on markers of oxidative stress: an Ancillary analysis of the Alberta Physical Activity and Breast Cancer Prevention Trial

“…Exercise has generally been found to increase antioxidant enzyme capacity while decreasing lipid peroxidation and DNA damage markers in healthy populations of men and women. [39][40][41][42][43][44][45][46][47][48][49][50][51] However, not all studies showed consistency across biomarkers, 40 42 45 46 52 and some studies have shown no effect of exercise on oxidative stress or antioxidant capacity. 53 54 In these findings, study designs and populations are heterogeneous.…”

Spinning, oxidative damage and hemolysis in athletes

“…Immediately after spinning a significant increase in TBARS (thiobarbituric acid reactive substances) (from 16.5 AE 2 to 25 AE 2 nmol MDA/mL serum) was observed (p < 0.05) compared to both those at rest and control group (not regularly exercised). 9 The aim of this study is to test the effect of spinning load on oxidative damage (AAPH-induced MDA), oxidative hemolysis, RBC morphology study and relationship between athletes before and after exercise and in control samples and in AAPH treated samples (evaluating MDA levels).…”