Acute Effects of Tetracycline Exposure in the Freshwater Fish Gambusia holbrooki: Antioxidant Effects, Neurotoxicity and Histological Alterations

Nunes, Bruno; Antunes, Sara C.; Gomes, Rosana Canuto; Campos, João C.; Braga, Márcia R.; Ramos, A. S.; Correia, Alberto Teodorico

doi:10.1007/s00244-014-0101-z

Cited by 107 publications

(52 citation statements)

References 69 publications

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“…It was found at 295 μg kgG 1 after 10 days of continuous medication with the dosage of 75 mg kgG 1 . So, after 10 days all residues are not eliminated properly and substantial alteration were recorded in both kidney and liver tissues (Elia et al, 2014) and functioned as prooxidant (Nunes et al, 2015). Our finding indicates the same results we found all residues eliminated after 45 days from end of medication feeding (Fig.…”

Section: Discussionsupporting

confidence: 86%

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Effect of Oxytetracycline on Thai Silver Barb (Barbonymus gonionotus) and on it’s Culture Environment

Islam¹,

Rasul²,

Kashem³

et al. 2015

J. of Fisheries and Aquatic Science

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Studies were conducted to investigate impacts of oxytetracycline on Barbonymus gonionotus muscle. Oxytetracycline the most widely used antibiotics, was fed to Barbonymus gonionotus with 4 g kgG 1 rate through diet for 45 days. Growth rate was observed for different concentrations of oxytetracycline. Water quality parameters and bacterial quantification were observed for a period of 21 days. Pond water, sediment and fish muscle were tested before and after oxytetracycline medicated feeding. Before feeding of oxytetracycline medicated feed, there was no oxytetracycline in water, sediment and fish muscles. After 2 days of feeding the initial oxytetracycline accumulation was 5070.0 ppb, which reduced considerably to 1190.0 ppb after 20 days from end of medicated diet feeding and not detected after 42 days. Physico-chemical parameters of pond water were determined. Before treatment dissolve oxygen, pH and total hardness of fish pond was 4.516±0.25 mg LG 1 , 7.413±0.09 and 822.33±1.52 ppm, respectively. After 21 days of study period, reached to 5.27±0.025 mg LG 1

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Section: Discussionsupporting

confidence: 86%

“…5-6). Exposure to drug oxytetracycline could result in adaptive alterations, changes in tissue and cellular damages (Nunes et al, 2015). Liver tissue specimens of the control group show normal hepatocytes and sinusoidal architectures (Fig.…”

Section: Discussionmentioning

confidence: 99%

Effect of Oxytetracycline on Thai Silver Barb (Barbonymus gonionotus) and on it’s Culture Environment

Islam¹,

Rasul²,

Kashem³

et al. 2015

J. of Fisheries and Aquatic Science

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“…Azithromycin and erythromycin orally administrated to salmon Oncorhynchus tshawytscha did not cause histopathologically significant lesions in the any tissue including gills, liver and kidney (Fairgrieve, Masada, McAuley, Peterson, Myers & Strom ); nevertheless, cytoplasm vacuolization, cellular hypertrophy and focal necrosis in renal tubules caused by erythromycin were reported in the some species (Kiryu & Moffitt ; Moffitt & Kiryu ). Fumagilin caused focal hepatic and kidney necrosis in O. mykiss ( Laurén, Wishkovsky, Groff, Hedrick & Hinton ); tetracycline caused numerous histopathology, including necrosis, in the gills and liver of Gambusia holbrooki (Nunes, Antunes, Gomes, Campos, Braga, Ramos & Correia ); oxytetracycline caused necrosis in the renal tubules and hematopoietic tissue of anterior kidneys in Tinca tinca (Soler, Reja, García‐Rubio, Míguez & Roncero ) and Cyprinus carpio (Svobodová, Sudová, Nepejchalová, Cervinka, Vykusová, Modrá & Kolárová ), but no change in kidney histology was detected in Piaractus mesopotamicus (Carraschi, Cruz, Machado Neto, Ignácio, Barbuio & Machado ). These data suggest that the toxic effects of a given therapeutic compound vary with specie sensitivity.…”

Section: Discussionmentioning

confidence: 99%

Effects of azithromycin on tilapia (Oreochromis niloticus): health status evaluation using biochemical, physiological and morphological biomarkers

Shiogiri¹,

Ikefuti²,

Carraschi³

et al. 2016

Aquac Res

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Bacterial diseases cause tilapia's high‐mortality outbreak. This study investigated the toxicity of azithromycin (AZT), a macrolide antibiotic that has been considered a possible therapeutic drug for tilapia aquacultural use. The 48‐h acute toxicity (50% lethal concentration, LC50; 48 h) of AZT was determined for Oreochromis niloticus. Thereafter, fish were exposed to 0, 1, 50 and 100 mg L−1 AZT during 14 days (chronic exposure) and measured the haematological variables, the activity of superoxide dismutase, catalase, glutathione peroxidase, glutathione‐S‐transferase (GST) and the concentration of glutathione (GSH), protein carbonyl and lipid peroxidation in the liver; histopathology was analysed the liver, gills and kidneys. The LC50; 48 h was >100 mg L−1. No fish died during chronic exposure. Haematocrit and haemoglobin concentration increased in fish exposed to 50 and 100 mg L−1, and the total number of leucocyte and thrombocyte increased after exposure to 100 mg L−1 AZT, suggesting a stimulation of defence cell production. In the liver, the antioxidant enzyme activities did not change, but GST activity and the GSH level increased in fish exposed to 100 mg L−1 AZT. Oxidative stress did not occur. Histopathological index (HIL) indicates moderate liver damage; minor histological changes in the gill and no change in the kidneys. AZT was considered non‐toxic for O. niloticus after acute exposure and, although it causes moderated histopathology in the liver after chronic exposure, this antibiotic may be an alternative against bacterial infections, depending on its efficacy to control bacterial disease in fish.

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“…A large number of previous projects developed by our work team used fish collected at this same locations, without much variation among biological responses measured (see Brandão et al 2013;Nunes et al 2014Nunes et al , 2015. Being field organisms, all efforts were made (separation of males and immature females with the required size/age from the rest of the animals, and above all, a period of acclimatization/quarantine/depuration) to ensure results homogeneity (see below).…”

Section: Methodsmentioning

confidence: 99%

Ecotoxicological effect of zinc pyrithione in the freshwater fish Gambusia holbrooki

et al. 2015

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Currently diverse biocidal agents can be used for distinct applications, such as personal hygiene, disinfection, antiparasitic activity, and antifouling effects. Zinc pyrithione is an organometallic biocide, with bactericidal, algicidal and fungicidal activities. It has been recently incorporated in antifouling formulas, such as paints, which prevent the establishment of a biofilm on surfaces exposed to the aquatic environment. It has also been used in cosmetics, such as anti-dandruff shampoos and soaps. Previously reported data has shown the presence of this substance in the aquatic compartment, a factor contributing to the potential exertion of toxic effects, and there is also evidence that photodegradation products of zinc pyrithione were involved in neurotoxic effects, namely by inhibiting cholinesterases in fish species. Additional evidence points to the involvement of zinc pyrithione in alterations of metal homeostasis and oxidative stress, in both aquatic organisms and human cell models. The present work assesses the potential ecotoxicity elicited by zinc pyrithione in the freshwater fish Gambusia holbrooki after an acute (96 h) exposure. The oxidative stress was assessed by the quantification of the activities of specific enzymes from the antioxidant defense system, such as catalase, and glutathione-S-transferases; and the extent of peroxidative damage was quantified by measuring the thiobarbituric acid reactive substances levels. Neurotoxicity was assessed through measurement of acetylcholinesterase activity; and a standardized method for the description and assessment of histological changes in liver and gills of was also used. Zinc pyrithione caused non-specific and reversible tissue alterations, both in liver and gills of exposed organisms. However, histopathological indices were not significantly different from the control group. In terms of oxidative stress biomarkers, none of the tested biomarkers indicated the occurrence of pro-oxidative effects, suggesting that the oxidative pathway is not the major toxicological outcome of exposure to zinc pyrithione.

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Acute Effects of Tetracycline Exposure in the Freshwater Fish Gambusia holbrooki: Antioxidant Effects, Neurotoxicity and Histological Alterations

Cited by 107 publications

References 69 publications

Effect of Oxytetracycline on Thai Silver Barb (Barbonymus gonionotus) and on it’s Culture Environment

Effect of Oxytetracycline on Thai Silver Barb (Barbonymus gonionotus) and on it’s Culture Environment

Effects of azithromycin on tilapia (Oreochromis niloticus): health status evaluation using biochemical, physiological and morphological biomarkers

Ecotoxicological effect of zinc pyrithione in the freshwater fish Gambusia holbrooki

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