Acute electrophysiological responses of bradykinin‐stimulated human fibroblasts.

Estacion, Mark

doi:10.1113/jphysiol.1991.sp018569

Cited by 19 publications

(9 citation statements)

References 37 publications

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“…As is shown in the companion paper (Estacion, 1991), ion channels may be involved in the signal transduction response of human fibroblasts to a specific peptide mitogen. The possibility exists that different ion channels are involved depending on whether the signal codes for proliferation or for an action such as secretion.…”

Section: Possible Regulation Of Ion Channel Expressionmentioning

confidence: 99%

“…Experiments performed with GTPyS included in the pipette are described in the accompanying paper (Estacion, 1991). In cells that expressed inward rectifier, however, it was noticed that the inward rectifier current seemed to consistently 'wash-out' (n = 14) over a period of minutes.…”

Section: Voltage-gated Channels K+ Channelsmentioning

confidence: 99%

“…Possible roles for some of the ion channels are discussed. The characterization of the ion channels normally found in the fibroblast defines a baseline from which to detect mitogen-stimulated modulation described in the accompanying paper (Estacion, 1991). This paper includes currents characterized while performing control experiments for the addition of nucleotides to the pipette solutions used in the companion paper.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of ion channels seen in subconfluent human dermal fibroblasts.

Estacion¹

1991

The Journal of Physiology

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SUMMARY1. Ion channels expressed in human dermal fibroblasts are characterized using the patch-clamp technique.2. A number of different ion channels were found but their expression occurred at various frequencies. The most commonly found phenotype was the expression of voltage-gated K+ current. This 'typical' K+ current was seen in about 60% of the cells recorded.3. Subtypes of voltage-gated K+ channels could be discerned by differences in gating kinetics. One has fast inactivation and resembles the 'A' K+ current. Additional subtypes were sometimes discerned based on activation kinetics.4. The large-conductance Ca2+-activated K+ channel (maxi-K+) could be found in nearly every cell but required large depolarizations to activate using the standard Ca2l-buffered pipette solution (10-8 M [Ca2+]i).5. Inward rectifier K+ channels were seen in a low percentage of cells. The inward rectifier K+ current was sensitive to 'wash-out' if guanosine 5'-O-(3-thiotriphosphate) (GTPyS) was included in the pipette solution dialysing the cell.6. Tetrodotoxin (TTX)-sensitive voltage-gated Na+ channels were seen but in a lower number of cells recorded, about 20 %. Evidence for subtypes of Na+ channels were sometimes seen based on differences in gating kinetics.7. An ATP-dependent osmotically activated Cl-current was also found. This current showed some outward rectification but was otherwise voltage independent.8. In addition, a cell-to-cell contact-associated K+ current was described. This current was linear over the voltage ranges used and whose gating correlated with the existence of gap junctions.9. These currents were characterized to determine the baseline behaviour of unstimulated cells and to compare to bradykinin-stimulated cells described in the following paper. As unexcitable cells, human dermal fibroblasts are capable of expressing a surprising diversity of ion channel phenotypes and of ion channel modulations.

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Section: Possible Regulation Of Ion Channel Expressionmentioning

confidence: 99%

Section: Voltage-gated Channels K+ Channelsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of ion channels seen in subconfluent human dermal fibroblasts.

Estacion¹

1991

The Journal of Physiology

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“…Bk-induced PI metabolism has been shown to cause an increase in the intracellular Ca2+ concentration ([Ca"fIi) in various cell preparations (Estacion, 1991;Fasolato et al, 1988;Ogura ct al., 1990). Preliminary studies on cortical astrocytes have indicated that Bk is able to mobilize [Ca2+Ii in distinct sub-populations of cells (Gimpl et al, 1992;Reiser and Hamprecht, 1985).…”

Section: Introductionmentioning

confidence: 99%

Calcium‐mobilizing and electrophysiological effects of bradykinin on cortical astrocyte subtypes in culture

Stephens

Cholewinski

Wilkin³

et al. 1993

Glia

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The possible consequences of activating bradykinin (Bk) receptors on identified astrocyte subtypes derived from rat cortex have been investigated in terms of: 1) mobilization of intracellular Ca2+; and 2) electrophysiological response. Bk induced a rapid, transient rise in intracellular Ca2+ in 63% of cortical type-1-like astrocytes and 44% of type-2 astrocytes tested. Experiments involving Ca(2+)-free conditions suggested that the release occurred largely from internal stores in both astrocyte subtypes. Bk receptor activation resulted in an inward current in approximately 10% of each astrocyte subtype tested using the whole-cell patch clamp technique. The use of perforated patch recording confirmed a similar population of cells responsive to Bk. The Bk-induced current was associated with a decrease in membrane conductance. The reversal potential was close to the K+ equilibrium potential, consistent with the closure of K+ channels. The current demonstrated similar EC50 values and Hill coefficients and also a marked degree of desensitization for both astrocyte subtypes. Recordings from type-1-like astrocytes indicated that the receptor mediating the electrophysiological response was of the B2 subtype. The data were consistent with the functional translation of Bk receptor occupation to physiological responses in distinct sub-populations of cortical type-1-like and type-2 astrocytes.

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“…2C). Since many types of non-neuronal cells in the nervous system are reported to be responsive to bradykinin (2,3,6), effects of bradykinin on [Ca 2+ ] i were examined. Bradykinin applied at 1 μM caused a [Ca 2+ ] i rise in the cells not responding to the high K + stimulation (Fig.…”

Section: Effects Of Gymnopilin On Cultured Dorsal Root Ganglion Cellsmentioning

confidence: 99%

Gymnopilin-a substance produced by the hallucinogenic mushroom, Gymnopilus junonius-mobilizes intracellular Ca2+ in dorsal root ganglion cells

et al. 2012

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Gymnopilus junonius is a widely spread mushroom in Japan and well known as a hallucinogenic mushroom. Gymnopilin was purified from the fruiting body of G. junonius and was reported to act on the spinal cord and depolarize motoneurons. This is the only evidence that gymnopilin has a biological effect on animals and no mechanism of the action has been determined at all. In this study, we examined effects of gymnopilin on intracellular Ca . These results indicate that gymnopilin activated phospholipase C and mobilize Ca 2+ from the intracellular Ca 2+ store in non-neuronal cells from the DRG. This is the first report to show that gymnopilin directly acts on cells isolated from the mammalian nervous system.

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Acute electrophysiological responses of bradykinin‐stimulated human fibroblasts.

Cited by 19 publications

References 37 publications

Characterization of ion channels seen in subconfluent human dermal fibroblasts.

Characterization of ion channels seen in subconfluent human dermal fibroblasts.

Calcium‐mobilizing and electrophysiological effects of bradykinin on cortical astrocyte subtypes in culture

Gymnopilin-a substance produced by the hallucinogenic mushroom, Gymnopilus junonius-mobilizes intracellular Ca2+ in dorsal root ganglion cells

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