Acute embryonic exposure of zebrafish to permethrin induces behavioral changes related to anxiety and aggressiveness in adulthood

Nunes, Mauro Eugênio Medina; Schimith, Lúcia Emanueli; Costa-Silva, Dennis Guilherme da; Leandro, Luana Paganotto; Martins, Illana Kemmerich; Mello, Renata Siqueira de; Nunes, Frederico; Santer, Melania; Vieira, Paula; Posser, Thaís; Franco, Jeferson Luís

doi:10.1016/j.jpsychires.2019.11.006

Cited by 13 publications

(3 citation statements)

References 35 publications

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“…At sublethal concentrations, permethrin is found to induce hyperactivity in larval fish; for example, visible spasms have been observed in zebrafish larvae at 50 μg l −1 ( DeMicco et al 2010 ). Larval zebrafish Danio rerio exposed to 25 μg l −1 permethrin decreased thigmotaxis, defined here as the affinity of an organism to preferentially remain near the boundaries of an environment, as opposed to the center ( Nunes et al 2020 ). Thigmotaxis has been observed in other fish species ( Millot et al 2009 , Sharma et al 2009 ), and can be used as a measure of anxiety in larval fish.…”

Section: Introductionmentioning

confidence: 99%

Exposure to permethrin or chlorpyrifos causes differential dose- and time-dependent behavioral effects at early larval stages of an endangered teleost species

Mundy

Hartz

Fulton

et al. 2021

Endang. Species. Res.

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Pyrethroid and organophosphate pesticides are two of the most commonly used classes of insecticide worldwide. At sublethal concentrations, permethrin (a pyrethroid) and chlorpyrifos (an organophosphate) impact behavior in model fish species. We investigated behavioral effects of environmentally relevant concentrations of permethrin or chlorpyrifos on early larval delta smelt Hypomesus transpacificus, a Critically Endangered teleost species endemic to the San Francisco Bay Delta, California, USA. Using a photomotor behavioral assay of oscillating light and dark periods, we measured distance moved, turn angle, meander, angular velocity, rotations, thigmotaxis (time spent in the border versus center), and swim speed duration and frequency. The lowest concentrations of permethrin used in the tests (0.05 and 0.5 µg l-1) caused significant increases in distance moved at 72 and 96 h, respectively. At 48, 72, and 96 h of exposure, 5 µg l-1 of permethrin caused a hyperactive state in which the larvae significantly decreased thigmotaxis, quickly turning in short bouts of activity, characterized by significant increases in rotations and freezing events. Larvae exposed to 0.05 µg l-1 chlorpyrifos significantly increased thigmotaxis at 72 and 96 h. In response to 5 µg l-1 chlorpyrifos, larvae significantly increased velocity at 72 h exposure, and significantly increased freezing events at 96 h. Behavioral data on larval delta smelt exposed to contaminants present in their limited habitat have the potential to aid evaluations of the suitability of spawning and rearing habitats for this endangered species, thus improving conservation management strategies focused on this sensitive life stage.

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Section: Introductionmentioning

confidence: 99%

Exposure to permethrin or chlorpyrifos causes differential dose- and time-dependent behavioral effects at early larval stages of an endangered teleost species

Mundy

Hartz

Fulton

et al. 2021

Endang. Species. Res.

View full text Add to dashboard Cite

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“…Thigmotaxism is an evolutionarily conserved behavior that consists in strongly avoiding the center of an arena and preferring to be in proximity to the boundaries of a novel environment. This behavior is found in a wide range of species, including rodents, fish, and humans, and is commonly used as an index of anxiety. − Reduced thigmotaxis was reported in Zebra Danio, Inland Silversides, and Delta Smelt after exposure to bifenthrin or other pyrethroid insecticides. ,− The observed combination of hyperactivity and reduced thigmotaxis can have broad ecological consequences, including an increased risk of predation. Hyperactive organisms with reduced thigmotaxis are indeed susceptible to having a reduced ability to hide from visually hunting predators .…”

Section: Discussionmentioning

confidence: 99%

Bifenthrin, a Ubiquitous Contaminant, Impairs the Development and Behavior of the Threatened Longfin Smelt during Early Life Stages

Mauduit

Segarra

Sherman

et al. 2023

Environ. Sci. Technol.

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The Longfin Smelt (Spirinchus thaleichthys) population in the San Franscisco Bay/Sacramento-San Joaquin Delta (Bay-Delta) has declined to ∼1% of its pre-1980s abundance and, as a result, is listed as threatened under the California Endangered Species Act. The reasons for this decline are multiple and complex, including the impacts of contaminants. Because the spawning and rearing seasons of Longfin Smelt coincide with the rainy season, during which concentrations of contaminants increase due to runoff, we hypothesized that early life stages may be particularly affected by those contaminants. Bifenthrin, a pyrethroid insecticide commonly used in agricultural and urban sectors, is of concern. Concentrations measured in the Bay-Delta have been shown to disrupt the behavior, development, and endocrine system of other fish species. The objective of the present work was to assess the impact of bifenthrin on the early developmental stages of Longfin Smelt. For this, embryos were exposed to 2, 10, 100, and 500 ng/L bifenthrin from fertilization to hatch, and larvae were exposed to 2, 10, and 100 ng/L bifenthrin from one day before to 3 days posthatch. We assessed effects on size at hatch, yolk sac volume, locomotory behavior, and upper thermal susceptibility (via cardiac endpoints). Exposure to these environmentally relevant concentrations of bifenthrin did not significantly affect the cardiac function of larval Longfin Smelt; however, exposures altered their behavior and resulted in smaller hatchlings with reduced yolk sac volumes. This study shows that bifenthrin affects the fitness-determinant traits of Longfin Smelt early life stages and could contribute to the observed population decline.

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“…BHG-I was weaker than IT and ICA in increasing SSA, COD, and bone mineralized elements. Behavioral changes of zebrafish have been linked to chemical exposure (Pitt et al, 2018;Nunes et al, 2020). Behavior analyzer-Ethovision XT-made it possible to examine numerous motor events and facilitates quantitative analysis of behavior (Zheng et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Prediction and Verification of Epimedium Flavonoids With Different Glycosylation Numbers in Reversing Glucocorticoid-Induced Bone Formation Inhibition by Molecular Docking and Zebrafish

Jiang

Xiao

et al. 2022

Front. Environ. Sci.

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Glucocorticoids have been detected in environmental waters, and their biological potency has raised concerns on their impact on aquatic vertebrates especially fish. Numerous researches showed that the continuous and direct contact of aquatic vertebrates with glucocorticoid contaminants in environmental water will cause bone formation inhibition. The aim of this study is to predict and verify the effect of icaritin (IT), icariin (ICA), and baohuside-I (BHG-I) in reversing glucocorticoid-induced bone formation inhibition (GIBFI) by molecular docking and zebrafish model. We contrasted their activity in reversing GIBFI from their affinity to bone metabolism proteins (OPG, RANKL, BMP-2, BMP-4, Runx-2) by molecular docking. Subsequently, zebrafish model was adopted to evaluate their reverse effects on GIBFI. Alizarin red staining coupled with image quantification were performed to evaluate the effects of ICA, IT, and BHG-I on skeleton stained area (SSA) and cumulative optical density (COD). Inductively coupled plasma-mass spectrometry was applied to determine the contents of bone mineral elements (CBME, Mg, K, Ca, Fe, Zn) in zebrafish bones. Docking results showed the receptors (BMP-2, BMP-4, and Runx2) all combined well to ICA, while BHG-I bound well to OPG, the affinity between IT and the above targets were the weakest. Fortunately, IT, ICA, and BHG-I significantly increased the SSA, COD, and the contents of Ca compared with the model group (p < 0.05) in the order of IT>ICA>BHG-I. In conclusion, the glycosyl groups increased the H-bond affinity between flavonoids and target sites, which weakened bone formation. IT, BHG-I, and ICA all alleviated GIBFI, but their intensity order was IT>ICA>BHG-I.

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Acute embryonic exposure of zebrafish to permethrin induces behavioral changes related to anxiety and aggressiveness in adulthood

Cited by 13 publications

References 35 publications

Exposure to permethrin or chlorpyrifos causes differential dose- and time-dependent behavioral effects at early larval stages of an endangered teleost species

Exposure to permethrin or chlorpyrifos causes differential dose- and time-dependent behavioral effects at early larval stages of an endangered teleost species

Bifenthrin, a Ubiquitous Contaminant, Impairs the Development and Behavior of the Threatened Longfin Smelt during Early Life Stages

Prediction and Verification of Epimedium Flavonoids With Different Glycosylation Numbers in Reversing Glucocorticoid-Induced Bone Formation Inhibition by Molecular Docking and Zebrafish

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