Acute Endometritis due to Taylorella equigenitalis Transmission by Insemination of Cryopreserved Stallion Semen

Overview of spatio-temporal distribution inferred by multi-locus sequence typing of Taylorella equigenitalis isolated worldwide from 1977 to 2018 in equidae

“…Within Europe, ST16 included in CC2 has emerged as the dominant genotype with a crossborder circulation; it is composed of strains from Austria (2004), Belgium (2008), France (2004), Germany (1989-2009), Poland and Switzerland (2005-2008. ST46, the primary founder of CC8, seems to have the same profile as ST16 without any Polish strains, but it was found to be mainly composed of German strains plus the strain isolated in 2018 from the vaginal discharge of a French mare infected by cryopreserved stallion semen collected and processed in Germany in 2012 (Delerue et al, 2019). This Germanic origin could be extended to CC8, which is mainly composed of German strains first isolated in 15 1981.…”

“…The sequence data obtained from the online Taylorella MLST database and from the literature (Delerue et al, 2019;Hicks et al, 2018) were incorporated into the dataset. The sequences of every individual locus were trimmed to equivalent lengths, like in the Taylorella MLST database.…”

“…The present study focused on a large population of 367 T. equigenitalis strains to determine, through their MLST genotypes, their diversity and spatiotemporal distribution according to the geographical origin, year of isolation and equine breed. A hundred and nineteen strains were characterised using MLST, and the MLST sequence data of 248 additional strains were accessed from the online Taylorella MLST database and the literature (Delerue et al, 2019;Hicks et al, 2018). Despite the large selection of strains in terms of geographical origin, year of isolation and equine breed, one area of concern is the amount of emphasis put on the number of strains within the same ST.…”

“…A total of 367 T. equigenitalis strains isolated from horses, two ponies and two donkeys, were included in this study: 119 strains were obtained and characterised using MLST; the MLST sequence data for 242 strains were downloaded from the online Taylorella MLST database (https://pubmlst.org/taylorella/), and the MLST sequence data for six strains were obtained from Hicks et al (Hicks et al, 2018) (n = 4) and Delerue et al (Delerue et al, 2019) (n = 2).…”

Comparison of seven nucleic acid amplification tests for detection of <i>Taylorella equigenitalis</i>

“…CEM cases were first confirmed in Japan in 1980 [13] and spread to the Thoroughbred population, but was eradicated from Japanese Thoroughbred horses by 2010 as a result of comprehensive infestation control measures [1]. However, sporadic cases of CEM have been identified around the world in recent years [8,10,15]. The detection of T. equigenitalis through quarantine is most important in CEM-free countries such as Japan.…”