Acute enhancing effect of a standardized extract of Centella asiatica (ECa 233) on synaptic plasticity: an investigation via hippocampal long-term potentiation

Abstract

Context: ECa 233 is the standardized extract of Centella asiatica (L.) Urban. (Apiaceae). It contains at least 85% of triterpenoid glycosides and yields neuroprotective and memory-enhancing effects. However, the exact molecules exerting the effects might be triterpenic acid metabolites reproduced through gut metabolism after orally ingesting C. asiatica, not triterpenoid glycosides.Objective: This study demonstrates the effect of unmetabolized ECa 233 on hippocampal synaptic plasticity after directly perfusing ECa 233 over acute brain slices. Materials and methods: The brain slices obtained from 7-week-old male Wistar rats were randomly divided into 4 groups. We perfused either artificial cerebrospinal fluid (ACSF), 0.01% DMSO, 10 mg/mL ECa 233, or 100 mg/mL on brain slices, and measured the long-term potentiation (LTP) magnitude to determine the synaptic plasticity of hippocampal circuits in each group.

Optimization of Parkinson's disease therapy with plant extracts and nutrition’s evolving roles

“…Essentially, these compounds exert their effects through modulation of molecular pathways concerned with pathogenesis of PD, including mitochondrial dysfunction, neuroinflammation, and oxidative stress ( Haleagrahara and Ponnusamy, 2010 , Xu ChangLiang et al, 2012 ). Furthermore, CA has been shown to promote synaptic plasticity, neurogenesis, neuronal survival, and function in disease rodent brain regions and networks ( Omar et al, 2011 , Gray et al, 2016 , Sbrini et al, 2020 , Boondam et al, 2021 ).…”

Determination of the anticancer activity of standardized extract of Centella asiatica (ECa 233) on cell growth and metastatic behavior in oral cancer cells

“…It possesses the major bio-composition of madecassoside and asiaticoside, characterized as a white to off-white powder and a ratio of madecassoside to asiaticoside of 1.5 ± 0.5:1 ( 20 21 ). This plant has been reported to have various pharmacological properties and has been used for its asthma, ulcer, eczema, and wound healing effects, as well as memory and learning enhancement and anxiety relief, which can contribute to a deep state of relaxation and/or mental calmness throughout meditation practices ( 22 23 24 25 26 ). ECa 233 also acts an as inhibitor in the lipopolysaccharide-stimulated production of inflammatory mediators in keratinocyte HaCaT cells by suppressing ERK1/2 and nuclear factor kappa B pathways, which may be related to the inhibition of reactive oxygen species (ROS) production ( 27 ).…”