Acute Excisional Wounds Treated with a Tissue-Engineered Skin (Apligraf)

Eaglstein, William H.; Alvarez, Oscar M.; Auletta, Michael J.; Leffel, David; Rogers, Gary S.; Zitelli, John A.; Norris, James E; Thomas, Isabelle; Irondo, Manuel; Fewkes, Jessica L.; Hardin-Young, Janet; Duff, Ronald G.; Sabolinski, Michael L.

doi:10.1046/j.1524-4725.1999.08186.x

Cited by 123 publications

(85 citation statements)

References 10 publications

(12 reference statements)

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“…30,54 Type I collagen triggers immune reactions in only a low percentage of people, and a simple serologic test can determine whether a patient has it. 55,56 In addition, collagen and HA implants significantly inhibited the growth of bacteria pathogens that cause infections, compared with polylactic-co-glycolic acid implants. 57 However, for a prefabricated bioceramic implants such as HA to fit into a bone cavity, the surgeon needs to machine the graft to the desired shape or carve the surgical site, thus leading to increases in bone loss, trauma, and surgical time.…”

Section: Discussionmentioning

confidence: 99%

Collagen-Calcium Phosphate Cement Scaffolds Seeded with Umbilical Cord Stem Cells for Bone Tissue Engineering

Thein-Han

2011

Tissue Engineering Part A

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Human umbilical cord mesenchymal stem cells (hUCMSCs) avoid the invasive procedure required to harvest bone marrow MSCs. The addition of collagen fibers into self-setting calcium phosphate cement (CPC) may increase the scaffold strength, and enhance cell attachment and differentiation. The objectives of this study were to develop a novel class of collagen-CPC composite scaffolds, and to investigate hUCMSC attachment, proliferation, and osteogenic differentiation on collagen-CPC scaffolds for the first time. Collagen fibers in CPC improved the load-bearing capability. Flow cytometry showed that the hUCMSCs expressed cell surface markers characteristic of MSCs, and were negative for hematopoietic and endothelial cell markers. hUCMSCs proliferated rapidly in all CPC composite scaffolds, with cell number increasing by sevenfold in 8 days. Cellular function was enhanced with collagen fibers in CPC scaffolds. Cell density increased from (645 -60) cells/mm 2 on CPC with 0% collagen, to (1056 -65) cells/mm 2 on CPC with 8% collagen ( p < 0.05). The actin stress fibers inside the hUCMSCs were stained, and the fluorescence intensity was doubled when the collagen in CPC was increased by 0% to 8%. RT-PCR showed that hUCMSCs on CPC with collagen had higher osteogenic expression than those on CPC without collagen. Alizarin Red S staining revealed a great increase in mineralization by hUCMSCs on CPC with collagen than that without collagen. In conclusion, hUCMSCs showed excellent proliferation, differentiation, and synthesis of bone minerals in collagen-CPC composite scaffolds for the first time. The novel hUCMSC-seeded collagen-CPC construct with superior cell function and load-bearing capability is promising to enhance bone regeneration in a wide range of orthopedic and craniofacial applications.

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Section: Discussionmentioning

confidence: 99%

Collagen-Calcium Phosphate Cement Scaffolds Seeded with Umbilical Cord Stem Cells for Bone Tissue Engineering

Thein-Han

2011

Tissue Engineering Part A

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“…A tissue-engineered, Food and Drug Administration-approved, artificial skin containing fibroblast allografts harvested from neonatal skin samples has been available for treatment of nonhealing ulcers and other cutaneous defects. [25][26][27][28][29] Little or no deleterious immune response to the implanted cells has been documented with this device. 29 Recently, investigators from Japan 30 and South Korea 31 also reported research related to FBC coil embolization.…”

Section: Discussionmentioning

confidence: 99%

Endovascular Treatment of Experimental Aneurysms by Use of Fibroblast-Coated Platinum Coils

Dai

Ding

Danielson

et al. 2007

Stroke

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Background and Purpose-The purpose of this study was to determine whether implanting exogenous fibroblasts on platinum coils could enhance intra-aneurysmal fibrosis. Hypotheses included: (1) fibroblast-coated (FBC) platinum coils can improve angiographic results after embolization; and (2) FBC platinum coils can accelerate histological healing of embolized aneurysms. Methods-Experimental aneurysms in rabbits were embolized with control platinum coils (nϭ18) or FBC coils (nϭ18).Subjects were euthanized at 14 days, 1 month, 3 months and 6 months after implantation. Digital subtraction angiography was used to evaluate stability after embolization. Histological samples were examined with a grading system (range, 0 to 12) based on neck and dome healing. Results-Histology total scores and fibrosis ratio at 14 days were significantly greater in the FBC coil group compared with controls (6.6Ϯ1.9 versus 2.5Ϯ1.1, 1.2Ϯ0.6% versus 0.2Ϯ0.3%, respectively; Pϭ0.0090). Cavities embolized with FBC coils showed cellular proliferation and thrombus organization, with an endothelialized membrane bridging the neck. There were no differences between groups in the later timepoints. The FBC coil group showed radiographic stability in 11 (61%) cases, coil compaction in 2 (11%) cases, and progressive occlusion in 5 (28%) cases. No progressive occlusion was seen in controls; 3 (17%) of 18 control cases exhibited coil compaction (Pϭ0.0546). Conclusions-FBC

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“…Although this product does not cause immunological rejection, allogeneic cells of the construct do not survive after one to two months in vivo (Eaglstein et al 1999;Griffiths et al 2004). Hence, Apligraf, which was marketed initially as an organotypic skin substitute, can only be considered as a temporary bioactive dressing.…”

Section: Apligrafmentioning

confidence: 99%

A review of tissue-engineered skin bioconstructs available for skin reconstruction

Shevchenko

James

2009

J. R. Soc. Interface.

629

469

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Situations where normal autografts cannot be used to replace damaged skin often lead to a greater risk of mortality, prolonged hospital stay and increased expenditure for the National Health Service. There is a substantial need for tissue-engineered skin bioconstructs and research is active in this field. Significant progress has been made over the years in the development and clinical use of bioengineered components of the various skin layers. Off-the-shelf availability of such constructs, or production of sufficient quantities of biological materials to aid rapid wound closure, are often the only means to help patients with major skin loss. The aim of this review is to describe those materials already commercially available for clinical use as well as to give a short insight to those under development. It seeks to provide skin scientists/tissue engineers with the information required to not only develop in vitro models of skin, but to move closer to achieving the ultimate goal of an off-the-shelf, complete full-thickness skin replacement.

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Acute Excisional Wounds Treated with a Tissue-Engineered Skin (Apligraf)

Abstract: To date, this is the largest experience with a tissue-engineered skin product in acute wounds, and this study suggests that tissue therapy may be safe and useful.

Cited by 123 publications

References 10 publications

Collagen-Calcium Phosphate Cement Scaffolds Seeded with Umbilical Cord Stem Cells for Bone Tissue Engineering

Collagen-Calcium Phosphate Cement Scaffolds Seeded with Umbilical Cord Stem Cells for Bone Tissue Engineering

Endovascular Treatment of Experimental Aneurysms by Use of Fibroblast-Coated Platinum Coils

A review of tissue-engineered skin bioconstructs available for skin reconstruction

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