Acute exercise during hemodialysis prevents the decrease in natural killer cells in patients with chronic kidney disease: a pilot study

Fuhro, Maria Isabel; Dorneles, Gilson Pires; Andrade, Francini Porcher; Romão, Pedro R T; Peres, Alessandra; Monteiro, Mariane Borba

doi:10.1007/s11255-017-1747-z

Cited by 11 publications

(20 citation statements)

References 45 publications

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“…Zhang et al found that NK cells can directly damage renal tubular epithelial cells in vitro and that perforin is also involved in their cytotoxic acquisition and deeply involved in brosis during their evolution [35][36]. Similarly, the accumulation of NK cells in renal tubules leads to irreversible renal brosis [37][38][39]. This is consistent with our data suggesting a correlation between renal brosis and increased perforin and IFN-γ.…”

Section: Discussionsupporting

confidence: 91%

Rehin Mitigates Chronic Kidney Injury by Decreasing the Release of Perforin and IFN-γ from Immune Cells Via Inhibiting the STING/TBK1/IRF3 Pathway Activation

Wang

Huang

Liao³

et al. 2022

Preprint

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Shenkang suppository (SKS), a Chinese medicinal preparation rich in various natural ingredients, has not been reported in any studies related to fibrosis. Our experiments validated the anti-fibrotic and anti-inflammatory effects of Rhein (Rh), which is a major component of SKS, and explored its potential immune mechanisms. Tissue and serum specimens from chronic kidney disease (CKD) patients and normal subjects were collected in 30 cases each, and the expression differences of perforin and IFN-γ were analyzed by ELISA. Further, the CKD mice model constructed with folic acid (FA) was used to validate these differences by WB and qRT-PCR to explore the potential nephroprotective mechanism of Rh. Besides, in vitro experiments were conducted to identify the release sources of perforin and IFN-γ. ELISA showed that perforin and IFN-γ were upregulated in CKD patients, and this phenomenon was also corroborated in CKD mice. WB and qRT-PCR data showed that Rh reversed perforin and IFN-γ upregulation, inflammatory factor recruitment, and extracellular matrix (ECM) protein upregulation. Results from in vitro experiments demonstrate that the upregulation of perforin and IFN-γ originates from the stress response of CD4+ T lymphocytes (CD4+ cells), CD8+ T lymphocytes (CD8+ cells) and natural killer cells (NK cells), which can be suppressed by Rh. More importantly, the activated STING/TBK1/IRF3 pathway in CKD was also inhibited by Rh. Our data suggest that Rh possesses anti-fibrotic and nephroprotective effects, which mechanistically are associated with decreased release of perforin and IFN-γ from immune cells, which may be achieved by suppressing the STING/TBK1/IRF3 pathway.

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Section: Discussionsupporting

confidence: 91%

Rehin Mitigates Chronic Kidney Injury by Decreasing the Release of Perforin and IFN-γ from Immune Cells Via Inhibiting the STING/TBK1/IRF3 Pathway Activation

Wang

Huang

Liao³

et al. 2022

Preprint

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“…In this context, Fuhro et al 24 reported that intradialytic exercise does not have the additional effect of removing systemic solutes (i.e., urea and creatinine) nor does it alter C-reactive protein levels, corroborating our findings.…”

Section: Discussionsupporting

confidence: 90%

The effects of aerobic exercise on biochemical parameters in individuals with CKD on hemodialysis: A longitudinal study

Oliveira

Morais

Abreu

et al. 2020

jhgd

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Background: Chronic kidney disease is directly related to cardiovascular disorders. Guided physical exercises significantly improve the adverse effects of dialytic treatment. Objective: To analyze changes in biochemical parameters of subjects with chronic kidney disease undergoing moderate exercise during hemodialysis. Methods: This is an experimental study composed of 54 subjects submitted to hemodialysis, split into a control group and a group with intervention. The experimental group underwent three weekly sessions of aerobic exercise, performed during hemodialysis sessions, with a duration of 30 minutes, for 12 weeks. The blood parameters of both groups were compared. Results: Statistically significant differences were observed between pre (p=0.001) and post-exercise protocol for urea (p=0.006), calcium (p=0.001), alanine aminotransferase (p=0.020) and sodium (p=<0.001). In the control group, we observed significant differences for the calcium variable (p<0.001), alanine aminotransferase (p=0.024), hematocrit (p=0.015), calcium vs phosphorus (p=0.018), and sodium (p=0.023), before and after the period. Conclusion: Aerobic training during hemodialysis was able to maintain blood level stability in patients with chronic kidney disease, both during and at the end of the protocol, even considering increased blood flow. This trial is registered in the Brazilian registry of clinical Trials - number RBR-7354r6. Registration date: July 5, 2018 at 12:59 PM. Last Update: July 24, 2018 at 10:24 AM. Identification of the test - UTN Number: U1111-1216-8272.

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“…Previously published data reported the peripheral frequency of Natural Killer cells and cytokine levels in response to intradialytic exercise. 7,9 The non-inclusion criteria included patients with severe heart disease, uncontrolled arterial hypertension, thrombosis, ischemic cardiopathy, amputation, excessive pallor, severe dyspnea, femoral fistula, precordial pain, orthopedic or neurological compromising, and cognitive impairment. Additionally, eight healthy elderly (age between 60 and 80 years) individuals with no apparent diseases (i.e.…”

Section: Methodsmentioning

confidence: 99%

“…6 The effects of intradialytic exercise also impact inflammatory cytokines and the modulation of the peripheral proportions of several immune cells. 6–8 Here, we aimed to evaluate the effects of a single session of intradialytic moderate-intensity exercise on CCR5+ monocytes and lymphocytes of elderly individuals with CKD.…”

Section: Introductionmentioning

confidence: 99%

The impact of intradialytic exercise on immune cells expressing CCR5+ in patients with chronic kidney disease: A cross-over trial

Fuhro¹,

Andrade

Dorneles

et al. 2021

Int J Artif Organs

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The C-C chemokine receptor type 5 (CCR5) plays a role in the immunopathogenesis of chronic kidney disease (CKD). Exercise has anti-inflammatory properties that may contribute to the rehabilitation of CKD patients. To date, the impact of the intradialytic exercise on CCR5 expression in monocytes and lymphocytes of CKD patients is unknown. We aimed to evaluate the effects of an acute intradialytic moderate-intensity exercise on CD4+CCR5+ T-cells and CD14+CCR5+ monocytes of elderly individuals with Chronic Kidney Disease (CKD). Eight CKD elderly patients performed a single bout of 20 min intradialytic exercise and a control hemodialysis (HD) session. Blood samples were collected at baseline, during and immediately after the trials. HD therapy increased the peripheral frequency of CD4+CCR5+ T-cells. The systemic CCL5 levels and the peripheral CD14+CCR5+ proportions increased during and after HD therapy. No significant alterations in CD4+CCR5+ and CD14+CCR5+ proportions or CCL5 levels were identified in CKD patients during and after intradialytic exercise. A negative correlation between the peripheral frequency of CD14+CCR5+ and the creatinine levels was identified in the intradialytic exercise session. A single moderate-intensity intradialytic exercise imposes an immunomodulatory impact in CKD elderly patients, preventing an excessive inflammatory response induced by hemodialysis.

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Acute exercise during hemodialysis prevents the decrease in natural killer cells in patients with chronic kidney disease: a pilot study

Abstract: This study suggests the potential role of intradialytic exercise to prevent the decrease in peripheral frequency of NK cell subsets during HD therapy. Moreover, moderate intensity intradialytic exercise did not exacerbate the systemic inflammation or induce muscle damage during HD therapy.

Cited by 11 publications

References 45 publications

Rehin Mitigates Chronic Kidney Injury by Decreasing the Release of Perforin and IFN-γ from Immune Cells Via Inhibiting the STING/TBK1/IRF3 Pathway Activation

Rehin Mitigates Chronic Kidney Injury by Decreasing the Release of Perforin and IFN-γ from Immune Cells Via Inhibiting the STING/TBK1/IRF3 Pathway Activation

The effects of aerobic exercise on biochemical parameters in individuals with CKD on hemodialysis: A longitudinal study

The impact of intradialytic exercise on immune cells expressing CCR5+ in patients with chronic kidney disease: A cross-over trial

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