Acute exposure to environmentally relevant concentrations of copper affects branchial and hepatic phosphoryl transfer network of Cichlasoma amazonarum: Impacts on bioenergetics homeostasis

Direct and delayed synergistic effects of marine heatwaves, metals and food limitation on tropical reef-associated fish larvae

“…We found strong and clear dose-dependent effects of Cu on all measured response variables of P. waigiensis larvae, including reduced survival, growth rate, and feeding. Cu is highly toxic to fish as it can increase cellular oxidative damages while impairing enzyme activities and repairing process and disrupting homeostasis (Baldissera et al, 2020; Braz-Mota et al, 2017; Xu et al, 2018), impairing behaviours (McIntyre et al, 2012; Sovova et al, 2014), and increased mortality (Braz-Mota et al, 2017). This is alarming as in this study, the exposure Cu concentrations of 100 - 175 µg Cu L -1 are lower than a limited Cu concentration of 200 µg L -1 allowed for the surface water for aquaculture purposes in Vietnam (MONRE-Vietnam, 2015) and fall within the measured Cu concentrations in coastal waters in the tropics (Jonathan et al, 2011; Le and Nguyen, 2018).…”

Creatine Kinase Activity as an Indicator of Energetic Impairment and Tissue Damage in Fish: A Review

“…The determination of CK activity in serum or plasma has been commonly used to evaluate tissue damage, since CK is released into the bloodstream during damage (Table 2). Oreochromis niloticus Bacterial infection (Providencia rettgeri) Gills Inhibition of cytosolic and mitochondrial CK activities [10] Ctenopharyngodon idella Fungal infection (Saprolegnia parasitica) Gills Inhibition of cytosolic and mitochondrial CK activities [11] Rhamdia quelen Exposure to organophosphate trichlorfon Gills Inhibition of cytosolic and mitochondrial CK activities [12] Rhamdia quelen Exposure to organophosphate trichlorfon Muscle Inhibition of cytosolic and mitochondrial CK activities [13] Cichlasoma amazonarum Exposure to copper Gills Inhibition of mitochondrial CK activity; increase in cytosolic CK activity [14] Ctenopharyngodon idella Exposure to methylmercury chloride Gills Inhibition of cytosolic and mitochondrial CK activities [15] Lophiosilurus alexandri Hypoxia Gills Inhibition of cytosolic and mitochondrial CK activities [16] Oreochromis niloticus Hypoxia Gills Inhibition of cytosolic and mitochondrial CK activities [17] Brycon amazonicus Air exposure Gills Inhibition of cytosolic and mitochondrial CK activities [18] Table 2. The use of serum/plasma creatine kinase (CK) activity as indicator of fish tissue damage.…”

“…The population growth and consequential increase in the release of domestic, industrial, and mining residues into the environment has increased metal contamination, which has aroused public concern due to their prolonged persistence in the environment, toxicity, and tendency towards bioaccumulation [33]. Exposure to relevant concentrations of copper (750 and 1500 ug/L) for 96 h affected branchial bioenergetic homeostasis in Cichlasoma amazonarum due to the decrease in ATP levels elicited by the disruption of mitochondrial CK activity [14]. The same authors demonstrated that these waterborne Cu levels impaired the energetic balance associated with ATP metabolism of the gills, an organ with high and variable energy requirements, through the inhibition of key enzymes of cellular and tissue homeostasis.…”