Acute hemodynamic changes following hemorrhage and volume restitution, using a low viscosity plasma expander, in anesthetized portal hypertensive rats

Systemic and splanchnic hemodynamics following hemorrhage and volume restitution with Haemaccel in portal hypertensive rats: The effect of propranolol

“…Haemaccel is a commercially available, colloid solution, a product of gelatin 7 (Aventis‐Pharma, Marbourg, Germany) that is used as a plasma volume expander in the treatment of hemorrhagic shock. The viscosity of Haemaccel is lower than the viscosity of blood, and volume replacement with Haemaccel reduces blood viscosity 6 . We found that in this model, Haemaccel induced an increase in cardiac output and portal venous inflow, thus preventing the reduction in portal pressure, which might be expected when viscosity is reduced 6 …”

“…Portal hypertension was induced by portal vein stenosis. The operative procedure for production of portal hypertension has been previously described 6,11,12 . The animals were anesthetized with ketamine HCl (150 mg/kg, i.m.).…”

“…Hemodynamic studies were performed by using the radioactive microsphere technique. The technique used for the hemodynamic measurements has been detailed previously 6,12 . Animals were anesthetized with ketamine‐HCl (150 mg/kg, i.m.)…”

“…Blood was withdrawn from the femoral catheter into a heparinized syringe by the use of a Harvard pump (Harvard Apparatus, Millis, MA, USA), at a constant rate of 0.3 mL/min for 15 min. The amount of blood withdrawn was approximately 15 mL/kg bodyweight, which is 19% of the calculated blood volume (assumed to be 8% of bodyweight) 3,6 . This was followed by 15 min of stabilization.…”

“…However, depletion of blood volume lowers portal pressure, 2 while blood volume expansion increases portal pressure 3 and occasionally precipitates hemorrhage 4,5 . Recently, we studied the acute hemodynamic effects of hemorrhage and volume restitution with Haemaccel in a rat model with prehepatic portal hypertension 6 . Haemaccel is a commercially available, colloid solution, a product of gelatin 7 (Aventis‐Pharma, Marbourg, Germany) that is used as a plasma volume expander in the treatment of hemorrhagic shock.…”