2016
DOI: 10.1155/2016/7260562
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Acute Hypoxic Stress Affects Migration Machinery of Tissue O2‐Adapted Adipose Stromal Cells

Abstract: The ability of mesenchymal stromal (stem) cells (MSCs) to be mobilised from their local depot towards sites of injury and to participate in tissue repair makes these cells promising candidates for cell therapy. Physiological O2 tension in an MSC niche in vivo is about 4–7%. However, most in vitro studies of MSC functional activity are performed at 20% O2. Therefore, this study focused on the effects of short-term hypoxic stress (0.1% O2, 24 h) on adipose tissue-derived MSC motility at tissue-related O2 level. … Show more

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Cited by 14 publications
(8 citation statements)
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“…While there were no enriched GO categories or KEGG pathways in the down-regulated gene set, we also further investigated these genes through a literature search. We found that a number of these genes are known to be down-regulated in response to hypoxia in human cells, such as PLA2G4C [66] and MACF1 [67]. We also found that some of these genes are potentially related to immune response such as HNRNPR which positively regulates MHC class 1 expression [68], DOCK8 which is important in the functioning of natural killer cells and receptor-γt-positive innate lymphoid cells [69,70], and ELF1 which plays a role in the functioning and development of lymphocytes [71].…”
Section: Resultsmentioning
confidence: 99%
“…While there were no enriched GO categories or KEGG pathways in the down-regulated gene set, we also further investigated these genes through a literature search. We found that a number of these genes are known to be down-regulated in response to hypoxia in human cells, such as PLA2G4C [66] and MACF1 [67]. We also found that some of these genes are potentially related to immune response such as HNRNPR which positively regulates MHC class 1 expression [68], DOCK8 which is important in the functioning of natural killer cells and receptor-γt-positive innate lymphoid cells [69,70], and ELF1 which plays a role in the functioning and development of lymphocytes [71].…”
Section: Resultsmentioning
confidence: 99%
“…Proliferative activity was assessed as population doubling time (PDT), measured in hours, and was calculated according to the following equation: PDT = (T-T0)*log 2/(log N-log N0), where (T-T0) is the duration of experiment (in hours), N is a number of cells at the end of the experiment, and N0 is a number of cells seeded initially. [22][23][24] Cells were seeded at standard density (3 × 10 3 cells/cm 2 ). Cell number was determined using a hemocytometer at day 0 and day 7.…”
Section: Adipose Tissue-derived Stromal Cells Proliferationmentioning
confidence: 99%
“…We successfully demonstrated that tissue O 2 ‐adapted ASCs are resistant to short‐term hypoxia, which can ensure their effective involvement in the regeneration of tissue damage under significant oxygen deprivation (Andreeva, Lobanova, Udartseva, & Buravkova, ). Further study revealed an attenuation of targeted and non‐targeted migration of ASCs after O 2 deprivation (Udartseva et al, ). These data appeared to be quite surprising as earlier acute hypoxic exposure of ambient (20%) O 2 ‐adapted MSCs was shown to enhance migration rate (Buravkova, Andreeva, Gogvadze, & Zhivotovsky, ).…”
Section: Discussionmentioning
confidence: 99%