Acute inflammatory response induced byHelicobacter pyloriin the rat air pouch

Abstract

Infection by Helicobacter pylori elicits persistent neutrophil infiltration in the gastric mucosa and stimulates the release of substances that may contribute to the establishment of gastritis. In this study, we used the rat air pouch model to evaluate the acute inflammatory response to H. pylori, in vivo. A pronounced neutrophil infiltration was observed 6 h and 12 h after the injection of H. pylori into the air pouch. Strains with different genotypes were able to induce cellular influx. This response was dependent upon the amount of bacteria injected and still occurred when heat-killed bacteria were employed. An increase in prostaglandin E(2) levels was observed, indicating that H. pylori induced cyclooxygenase 2 in this model. The production of interleukin-1 beta and tumor necrosis factor-alpha by leukocytes was also enhanced, suggesting that this model may be useful for studying the direct activation of neutrophils by H. pylori in vivo.

Resveratrol as a bioenhancer to improve anti-inflammatory activities of apigenin

“…Carrageenan-induced mice hind paw edema has been widely used for the discovery and evaluation of anti-inflammatory drugs because the relative potency estimates obtained tend to reflect clinical experience. This model allows quantitative assessment of both inflammation and the formation of chemical mediators such as cytokines [28]. As previously observed in in vitro experiment, acute inflammation being measured by paw volume was significantly suppressed when apigenin and resveratrol were co-administrated in mice before subcutaneous injection of carrageenan in hind paw (Figure 4).…”

Simultaneous determination of liquiritin, hesperidin, and glycyrrhizin by HPLC-photodiode array detection and the anti-inflammatory effect of Pyungwi-san

“…This model allows quantitative assessment of both inflammation and the formation of chemical mediators such as cytokines (Gambero et al, 2003). The anti-inflammatory effect of PWS was evaluated in vivo, using carrageenin-induced paw edema in SD rats.…”

The effects of 5HT3 receptor antagonist granisetron on inflammatory parameters and angiogenesis in the air-pouch model of inflammation

“…After 2 days the pouches were re-inflated with 10 ml of air. On day 6, inflammation was induced by injection of 1 ml of a 1% (w/v) suspension of carrageenan (Sigma, USA) in saline into the air-pouch under light diethyl ether anesthesia [25]. The carrageenan solution had been sterilized by autoclaving at 121°C for 15 min and supplemented with antibiotics [0.1 mg of penicillin G potassium (Jaber Ebn-e-Hayyan, Iran) and 0.1 mg streptomycin sulfate (Jaber Ebn-e-Hayyan, Iran) per milliliter of the solution] after cooling to 40-45°C.…”