Acute melatonin and para-chloroamphetamine interactions on pineal, brain and serum serotonin levels as well as stress hormone levels

Manzana, Ehrine J. P.; Chen, Wei Jung A; Champney, Thomas H.

doi:10.1016/s0006-8993(01)02656-7

Cited by 6 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At neuroendocrine level AMPH increased in a dose-dependent manner plasma corticosterone levels but had no effects on circulating catecholamine levels. Effects of chronic AMPH on 5-HT and 5-HIAA contents in the hypothalamus are in agreement to previously reported for methamphetamine (MA) (15,16) or AMPH (13) and several observations suggest that serotonergic neurons are responsible of the corticosterone. Serotonin would stimulate CRF release from hypothalamic neurons producing an increase of plasma ACTH and corticosterone (13).…”

Section: Discussionsupporting

confidence: 91%

Hematological, immunological and neurochemical effects of chronic amphetamine treatment in male rats

Llorente-García

Abreu-González

González-Hernández

2009

J. Physiol. Biochem.

View full text Add to dashboard Cite

In the present study, we have analyzed the effect of chronic amphetamine sulfate (AMPH) treatment on haematological, immunological and neurochemical parameters in the male rat. AMPH increased the total peripheral leukocyte count, and altered its differential counts, decreasing lymphocytes and increasing neutrophils. Flow cytometry study showed that the decline in circulating lymphocytes was caused by the loss of a particular lymphocyte subset, B-cell, which reduced both in percentage and in absolute number by 50%. T-cell population increased by 15% but not in absolute number, however there was no difference in either CD4+ or CD8+ T lymphocyte subsets between experimental groups. Neurochemically, AMPH reduced norepinephrine (NE) and serotonin (5-HT) contents in the hypothalamus and increased dopamine (DA) content in the striatum. Chronic AMPH increased in a dose-dependent manner serum corticosterone levels, had no effect on circulating catecholamines, reduced adrenal weights, and did not affect spleen weights although reduced their cellularities. These results show that chronic AMPH have important effects on immune function, particularly on humoral immune response because it reduced the circulating B cell population by half. In addition, AMPH plays an important role in the redistribution and trafficking of leukocytes, and both effects seem to be mediated by sympathetic innervation of the lymphoid organs.

show abstract

Section: Discussionsupporting

confidence: 91%

Hematological, immunological and neurochemical effects of chronic amphetamine treatment in male rats

Llorente-García

Abreu-González

González-Hernández

2009

J. Physiol. Biochem.

View full text Add to dashboard Cite

show abstract

“…Moreover a decrease in 5-HT release from synaptosomes of the preoptic and anterior hypothalamic areas was reported after a single dose of MLT (0.5 mg/kg). However, no effect on 5-HT content after MLT administration at high doses (ranging from 10–50 mg/kg) was found in whole mouse brain or in rat cortex, brain stem, pineal gland, or serum (Manzana et al, 2001; Sugden, 1983), although following administration of an even higher dose (60 mg/kg) a decrease in 5-HT release in the hypothalamus, corpus striatum and nucleus accumbens has been reported (Chuang and Lin, 1994). Based on the above-mentioned studies, a lower dose of MLT induces an accumulation of 5-HT in discrete brain areas, but decreased release in synaptic terminals.…”

Section: Discussionmentioning

confidence: 99%

Short-term effects of melatonin and pinealectomy on serotonergic neuronal activity across the light–dark cycle

et al. 2011

View full text Add to dashboard Cite

Melatonin (MLT) and serotonin (5-HT) are two biosynthetically related compounds implicated in several common physiological functions and the etiology of mood disorders. How they interact, though, is not yet fully understood. In this study, single-unit extracellular recordings were used to monitor dorsal raphe nucleus (DR) 5-HT neuronal activity in anesthetized rats, under basal conditions (CTRL), in response to MLT administration, and after pinealectomy (PX) across the light-dark cycle. Under basal conditions, the number of spontaneously active 5-HT neurons and their firing rate were both significantly lower in the dark phase. In the light phase, administration of MLT at low doses (0.5-1 mg/kg, i.v.) decreased 5-HT firing activity. This inhibitory effect of MLT was completely blocked by the MT₁/MT₂ receptor antagonist luzindole, but not by the selective MT(2) receptor antagonist 4P-PDOT, the selective 5-HT(1A) receptor antagonist WAY100635, or by the α₂ adrenoceptor antagonist idazoxan. In the opposite experiment, PX increased 5-HT firing activity in the dark phase, and this was reversed by MLT administration (1 mg/kg, i.v.). Finally, in a forced swim test, MLT (1 mg/kg, i.p.) increased immobility time and decreased swimming behavior. Together, these results suggest that nocturnal MLT secretion imposes tonic inhibitory control over a sub-population of DR 5-HT neurons. This MLT-induced decrease in 5-HT neurotransmission may represent a biological mechanism underlying mood disorders characterized by increased MLT secretion, such as seasonal affective disorder.

show abstract

“…Amphetamine, a psychostimulant drug, has been progressively more commonly abused in recent years; it acts in the central nervous system, causing damage to dopaminergic, adrenergic, and serotonergic nerve terminals in many brain areas [11–13]. Growing evidence has shown that acute AMPH exposure is toxic to the dopaminergic system, causing the release of excess dopamine from vesicles, inhibition of mitochondrial function, and the generation of reactive quinines, reactive oxygen species (ROS), and reactive nitrogen (RNS) species [14].…”

Section: Introductionmentioning

confidence: 99%

Melatonin reduces the expression of alpha‐synuclein in the dopamine containing neuronal regions of amphetamine‐treated postnatal rats

Sae-ung

Uéda

Govitrapong

et al. 2011

Journal of Pineal Research

View full text Add to dashboard Cite

Alpha-synuclein (α-syn) is a neuronal protein that is involved in various degenerative disorders such as Parkinson's disease. It is found in the presynaptic terminals and perinuclear zones of many brain regions. Amphetamine (AMPH), a psychostimulant drug abused progressively more commonly in recent years, has been known to induce neurotoxicity in the central dopaminergic pathway, which is associated with increased oxidative stress. Recently, AMPH has been shown to significantly increase the level of α-syn in dopaminergic neuroblastoma cell cultures. Melatonin is recognized as an antioxidant for the nervous system. This study tested whether melatonin can attenuate the effect of AMPH on the expression of α-syn in the dopaminergic pathway of the neonatal rat. Four-day old postnatal rats (P4) were injected subcutaneously with either AMPH (increasing dose, 5-10 mg/kg daily) alone or AMPH with melatonin (2 mg/kg) daily at 10:00 AM for 7 consecutive days. As determined using Western blot, the level of α-syn was significantly increased in the substantia nigra, dorsal striatum, nucleus accumbens, and prefrontal cortex of the AMPH-treated group, while melatonin treatment either prior to AMPH or alone decreased the accumulation of the protein to 77%, 96%, 78%, and 77% of the control value, respectively. Furthermore, an immunofluorescent study showed that the α-syn-immunoreactivity increased noticeably in the nuclei of cell bodies and nerve terminals of the AMPH-treated group. Again, melatonin lowered this immunoreactivity. These results indicate that melatonin has a direct or indirect effect in reducing the expression of α-syn in the postnatal rat. The exact mechanism of this mitigation should be further investigated.

show abstract

Acute melatonin and para-chloroamphetamine interactions on pineal, brain and serum serotonin levels as well as stress hormone levels

Cited by 6 publications

References 18 publications

Hematological, immunological and neurochemical effects of chronic amphetamine treatment in male rats

Hematological, immunological and neurochemical effects of chronic amphetamine treatment in male rats

Short-term effects of melatonin and pinealectomy on serotonergic neuronal activity across the light–dark cycle

Melatonin reduces the expression of alpha‐synuclein in the dopamine containing neuronal regions of amphetamine‐treated postnatal rats

Contact Info

Product

Resources

About