Acute metal stress in Populus tremula×P. alba (717‐1B4 genotype): Leaf and cambial proteome changes induced by cadmium²⁺

Abstract

The comprehension of metal homeostasis in plants requires the identification of molecular markers linked to stress tolerance. Proteomic changes in leaves and cambial zone of Populus tremula x P. alba (717-1B4 genotype) were analyzed after 61 days of exposure to cadmium (Cd) 360 mg/kg soil dry weight in pot-soil cultures. The treatment led to an acute Cd stress with a reduction of growth and photosynthesis. Cd stress induced changes in the display of 120 spots for leaf tissue and 153 spots for the cambial zone. It involved a reduced photosynthesis, resulting in a profound reorganisation of carbon and carbohydrate metabolisms in both tissues. Cambial cells underwent stress from the Cd actually present inside the tissue but also a deprivation of photosynthates caused by leaf stress. An important tissue specificity of the response was observed, according to the differences in cell structures and functions.

Effect of short-term cadmium stress on Populus nigra L. detached leaves

“…Durand et al (2010) observed a similar effect in poplar leaves upon Cd administration to intact plants for longer times of exposure. Authors hypothesized that alpha-tubulin decrease could reflect the negative impact of long term metal stress on the growth of leaves.…”

“…Cd content was measured as reported by Durand et al (2010) on digested leaves samples including petioles. In detail, dried material was milled to a fine powder (Grinder, IKA, Staufen, Germany) and then mineralized.…”

Proteomic study of β-aminobutyric acid-mediated cadmium stress alleviation in soybean

“…Peroxiredoxin is a thiol-specific peroxidase that detoxifies peroxides and also plays essential roles in enzyme activation and redox sensing [51,52]. The up-regulation of peroxiredoxin has been reported in Cd-challenged Populus [17] and rice [22]. Here, in Cd-stressed soybean plants without BABA pre-treatment, a higher abundance of antioxidant enzymes was not detected.…”

“…For example, a relatively low abundance of proteins involved in the photosynthetic electron transport chain and Calvin cycle has been reported in Cd-exposed poplar [18,19], Populus [17], and Thlaspi [57]. Our present proteomic analysis of soybean leaf proteins revealed high abundance of oxygen-evolving enhancer protein 1 and ribulose bisphosphate carboxylase small chain 1 in both nonprimed and BABA-primed leaves.…”

“…The "primed state" can also be induced by the pretreatment of plants with various natural and synthetic compounds [10], including β-aminobutyric acid (BABA), which is a potent inducer of plant defense mechanisms against infection by various pathogens [11,12], as well as abiotic stresses [8,13]. Proteomic techniques have been exploited by numerous researchers to study Cd stress-induced proteome changes in Arabidopsis [14][15][16], Populus [17][18][19][20], rice [21][22][23], soybean [24,25], and tomato [26]. Although Cao et al [13] investigated the role of BABA in enhancing Cd tolerance in Arabidopsis, the potential of chemical priming in mitigating heavy metal stress effects has not been explored in other crops.…”