Acute mobilization and migration of bone marrow-derived stem cells following anterior cruciate ligament rupture

Abstract

ACL rupture induced peripheral blood mobilization of MSCs and migration of intravenously-injected allogenic MSCs to the injured joint, where they localized in the synovium and quadriceps MTJ.

Metabolomic serum profiling after ACL injury in rats: A pilot study implicating inflammation and immune dysregulation in post‐traumatic osteoarthritis

“…Mannose is a monosaccharide involved in protein N‐glycosylation, which is integral to several physiologic functions including lectin‐mediated cell‐cell interactions. One hypothesis is that reduced serum mannose reflects consumption in N‐glycosylation associated with stem and immune cell trafficking to the injured joint, which we have observed in this model . Urita et al described decreased concentrations of N‐glycan species in both human and murine osteoarthritic articular cartilage and demonstrated a correlation between high mannose N‐glycans and chondrocyte production of matrix‐degrading enzymes MMP‐13 and ADAMTS‐5 in response to interleukin‐1α stimulation .…”

“…Further, Chen et al demonstrated that increasing kynurenine levels via indoleamine 2,3‐dioxygenase (IDO)‐targeted gene therapy promoted the resolution of collagen‐induced arthritis, primarily by apoptosis of T‐cells and reduction in synovial IL‐17. We previously demonstrated that our ACL injury model leads to acutely increased IL‐17 expression in synovium . Given the presently demonstrated reduction in tryptophan metabolites that could serve as AHR ligands in ACLR animals, we hypothesize that a pro‐inflammatory environment is favored in the acute period after injury.…”

“…Subcutaneous carprofen was administered for preemptive analgesia, and subcutaneous buprenorphine for post‐injury analgesia. Characterization studies of bony remodeling, articular cartilage degeneration with documented worsening histologic grade (OARSI score of ∼12 at 4 and 10 weeks), and acute stem cell migration have been published on the same rats utilized in this study and can be referenced for further information on downstream joint changes and cellular phenomena.…”

Intra-articular tracking of adipose-derived stem cells by chitosan-conjugated iron oxide nanoparticles in a rat osteoarthritis model

“…T. Maerz et al reported that the myotendinous junction (MTJ) of the quadriceps was also an important target in the acute mobilization of stem cells aer anterior cruciate ligament transection (ACLT). 39 Unfortunately, no signicant positive staining was found in the muscle or tendon, which was probably due to the difference in surgery and time. Surprisingly, abundant positive staining was found in part of the bone marrow cavity (Fig.…”

Oxymatrine exerts protective effects on osteoarthritis via modulating chondrocyte homoeostasis and suppressing osteoclastogenesis

“…ACLT is one of the most common sporting injuries and is associated with an increased risk for developing post‐traumatic osteoarthritis (PTOA) . The ACLT injury model has been shown to reliably reproduce the changes found in human osteoarthritic bone.…”