Acute Mono-Arthritis Activates the Neurohypophysial System and Hypothalamo-Pituitary Adrenal Axis in Rats

Nishimura, Haruki; Kawasaki, Makoto; Matsuura, Takanori; Suzuki, Hitoshi; Motojima, Yasuhito; Baba, Kazuhiko; Ohnishi, Hideo; Yamanaka, Yoshiaki; Fujitani, Teruaki; Yoshimura, Mitsuhiro; Maruyama, Takashi; Ueno, Hiroki; Sonoda, Shozo; Nishimura, Kazuaki; Tanaka, Kentarou; Sanada, Kenya; Onaka, Tatsushi; Ueta, Yoichi; Sakai, Akinori

doi:10.3389/fendo.2020.00043

Cited by 16 publications

(26 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We randomly divided the experimental rats into three groups (n = 5‐6 per group): Group A (Control): without any intra‐articular (IA) injection, administered anaesthesia; Group B (Saline): administered 0.05 mL of saline IA injection; and Group C (MIA): administered an IA injection of MIA (1 mg/0.05 mL per joint; Sigma, St Louis, MO, USA) in saline. NaCl or MIA IA injections were administered into the right hind knee joint as described previously 26 …”

Section: Methodsmentioning

confidence: 99%

“…To evaluate knee OA, three‐dimensional micro‐computed tomography (CT) images and gross images of the right knee joint were taken. In addition, ipsilateral knee transverse diameters were determined to assess knee joint swelling using a digital caliper, as described previously 26 . As a baseline, ipsilateral knee transverse diameters were also determined before IA injection.…”

Section: Methodsmentioning

confidence: 99%

“…The mechanical and thermal nociceptive threshold of ipsilateral hind paws were assessed. For the mechanical nociceptive threshold, a manual von Frey test was carried out with calibrated filaments (North Coast Medical, Morgan Hill, CA, USA) as described previously 25,26,51 . For the thermal nociceptive threshold, a hot plate test (52.5°C) was performed as described previously 52 .…”

Section: Methodsmentioning

confidence: 99%

“…Tissue preparation for FIHC and fluorescence microscopy of transgenic rats was conducted as described previously 26,44 . Following i.p.…”

Section: Methodsmentioning

confidence: 99%

“…Because both exogeneous and endogenous OXT/AVP systems play an important role in nociception, we investigated this system in various rat models of pain 21‐26 . OXT and AVP are primarily synthesised in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) in the hypothalamus.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The neurohypophysial oxytocin and arginine vasopressin system is activated in a knee osteoarthritis rat model

Nishimura

Kawasaki

Suzuki

et al. 2020

J Neuroendocrinology

Self Cite

View full text Add to dashboard Cite

Osteoarthritis (OA) causes chronic joint pain and significantly impacts daily activities. Hence, developing novel treatment options for OA has become an increasingly important area of research. Recently, studies have reported that exogenous, as well as endogenous, hypothalamic‐neurohypophysial hormones, oxytocin (OXT) and arginine‐vasopressin (AVP), significantly contribute to nociception modulation. Moreover, the parvocellular OXT neurone (parvOXT) extends its projection to the superficial spinal dorsal horn, where it controls the transmission of nociceptive signals. Meanwhile, AVP produced in the magnocellular AVP neurone (magnAVP) is released into the systemic circulation where it contributes to pain management at peripheral sites. The parvocellular AVP neurone (parvAVP), as well as corticotrophin‐releasing hormone (CRH), suppresses inflammation via activation of the hypothalamic‐pituitary adrenal (HPA) axis. Previously, we confirmed that the OXT/AVP system is activated in rat models of pain. However, the roles of endogenous hypothalamic‐neurohypophysial hormones in OA have not yet been characterised. In the present study, we investigated whether the OXT/AVP system is activated in a knee OA rat model. Our results show that putative parvOXT is activated and the amount of OXT‐monomeric red fluorescent protein 1 positive granules in the ipsilateral superficial spinal dorsal horn increases in the knee OA rat. Furthermore, both magnAVP and parvAVP are activated, concurrent with HPA axis activation, predominantly modulated by AVP, and not CRH. The OXT/AVP system in OA rats was similar to that in systemic inflammation models, including adjuvant arthritis; however, magnocellular OXT neurones (magnOXT) were not activated in OA. Hence, localised chronic pain conditions, such as knee OA, activate the OXT/AVP system without impacting magnOXT.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%