Acute nitrite exposure-induced oxidative damage, endoplasmic reticulum stress, autophagy and apoptosis caused gill tissue damage of grass carp (Ctenopharyngodon idella): Relieved by dietary protein

Liu, Hongju; Dong, Min; Jiang, Wei‐Dan; Wu, Pei; Liu, Yang; Jin, Xiao‐Wan; Kuang, Sheng‐Yao; Tang, Ling; Zhang, Lu; Li, Feng; Zhou, Xiao‐Qiu

doi:10.1016/j.ecoenv.2022.113994

Cited by 17 publications

(1 citation statement)

References 43 publications

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“…Gene Ontology (GO) enrichment analysis of the DEGs uncovered sustained alterations in the genes associated with peptidase activity, as well as those involved in the extracellular region and extracellular matrix, in response to water stress in juvenile Sebastes schlegelii. These findings are consistent with previous studies that documented damage to gill filaments, epithelial cell flattening, and mitochondrial changes following acute water stress [28]. In the BD vs. BC group, the most significantly enriched DEGs were linked to peptidase activity and the extracellular region, suggesting that the genes related to enzyme activities are among the primary responders to water stress [29].…”

Section: Discussionsupporting

confidence: 91%

Transcriptome Analysis of Juvenile Black Rockfish Sebastes schlegelii under Air Exposure Stress

Liu,

Zhang,

Wei

et al. 2024

Fishes

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The study aimed to uncover the molecular response of juvenile Sebastes schlegelii to air exposure stress by identifying differentially expressed genes (DEGs) that may underlie their anti-stress mechanisms. Juvenile Sebastes schlegelii were subjected to varying durations of air exposure stress. The total RNA was extracted from whole tissues and sequenced using the Illumina NovaSeq 6000 platform. The transcriptome data were analyzed to identify DEGs through pairwise comparisons across a control group and two experimental groups exposed to air for 40 s and 2 min 30 s, respectively. The comparative DEG analysis revealed a significant number of transcripts responding to air exposure stress. Specifically, 5173 DEGs were identified in the 40 s exposure group (BS) compared to the control (BC), 6742 DEGs in the 2 min 30 s exposure group (BD) compared to the control (BC), and 2653 DEGs when comparing the BD to the BS group. Notably, Gene Ontology (GO) analysis showed an enrichment of DEGs associated with peptidase activity and extracellular regions, suggesting a role in the organism’s stress response. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis pointed to the involvement of metabolic pathways, which are crucial for energy management under stress. The upregulation of protein digestion and absorption pathways may indicate a physiological adaptation to nutrient scarcity during stress. Additionally, the identification of antibiotic biosynthesis pathways implies a potential role in combating stress-induced infections or damage. The identified DEGs and enriched pathways provide insights into the complex anti-stress response mechanisms in juvenile Sebastes schlegelii. The enrichment of peptidase activity and extracellular region-related genes may reflect the initiation of tissue repair and immune response following air exposure. The connection between protein digestion and absorption pathways and anti-stress capabilities could be interpreted as a metabolic readjustment to prioritize energy-efficient processes and nutrient assimilation during stress. The role of antibiotic biosynthesis pathways suggests a defensive mechanism against oxidative stress or microbial invasion that might occur with air exposure.

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Section: Discussionsupporting

confidence: 91%