Acute oral toxicity study of polyherbal formulation: Constac granules

Abstract

BACKGROUND: Constipation is a common and frequently persistent gastrointestinal motility condition. It affects the economic burden, the standard of living, social interaction, and the capacity to carry out everyday tasks. This study aimed to evaluate the acute oral toxicity of Constac granules in Wistar rats. METHODS: A total of 12 rats were randomly divided into four groups of three each. Group 1 served as the control and received only distilled water, while groups 2 and 3 received doses of 2000 mg/kg body weight of Constac granules, and group 4 received doses of 5000 mg/kg body weight of Constac granules. The rats were observed for 14 days for any signs of toxicity. Necropsy was performed on all rats at the termination of the study and gross pathology was recorded. RESULTS: The results showed no clinical signs of toxicity or mortality observed in any of the control and treatment groups rats during the 14-day observation period. The LD50 value was estimated to be greater than 5000 mg/kg body weight. CONCLUSION: The acute oral toxicity study of Constac granules in Wistar rats showed no toxicity at doses up to 5000 mg/kg body weight. These findings showed that the oral administration of the Constac granules is safe to use.