Acute oral toxicity test and assessment of combined toxicity of cadmium and aflatoxin B1 in kunming mice

Zhao, Qing; Yang, Zhao-Si; Cao, Sanjie; Chang, Yung-Fu; Cao, Yuqin; Li, Jia-Bing; Yao, Zi-Xuan; Wen, Yiping; Huang, Xiaobo; Wu, Rui; Yan, Qigui; Huang, Yong; Ma, Xiaoping; Han, Xinfeng; Wu, Yinglong

doi:10.1016/j.fct.2019.110577

Cited by 30 publications

(11 citation statements)

References 42 publications

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“…Thirty male and female C57BL6/J mice (7 weeks old, 20 ± 2 g) were purchased from Charles River (Beijing, China) and were raised in specific pathogen-free conditions at room temperature (25 ± 2 °C) and under a 12-h light/dark cycle in the barrier facility of the Animal Center of Jiangnan University (Wuxi, China) [ 33 ]. All of the animals were fed standard chow and sterile water and were acclimatized for 1 week before the experiment began.…”

Section: Methodsmentioning

confidence: 99%

A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

et al. 2021

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In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous intestinal microorganisms with potential probiotic function, we conducted a comprehensive experimental analysis of DSM 2950 to determine its safety. This comprised a study of its potential virulence genes, antibiotic resistance genes, genomic islands, antibiotic resistance, and hemolytic activity and a 14-day test of its acute oral toxicity in mice. The results indicated no toxin-related virulence genes in the DSM 2950 genome. Most of the genomic islands in DSM 2950 were related to metabolism, rather than virulence expression. DSM 2950 was sensitive to most of the tested antibiotics but was tolerant of treatment with kanamycin, neomycin, clindamycin, or ciprofloxacin, probably because it possessed the corresponding antibiotic resistance genes. Oral acute toxicity tests indicated that the consumption of DSM 2950 does not cause toxic side effects in mice. Overall, the safety profile of DSM 2950 confirmed that it could be a candidate probiotic for use in food and pharmaceutical preparations.

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Section: Methodsmentioning

confidence: 99%

A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

et al. 2021

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“…Heavy metals such as nickel, arsenic, lead, chromium, mercury, and cadmium share the main target organ, liver, with AFs [96], and it is therefore important to research their interactions. In an in vivo study, AFB 1 and cadmium chloride showed an additive interaction in inducing acute oral toxicity in kunming mice [136]. A synergistic effect of AFB 1 and sodium arsenite has been observed, including cytotoxicity in vitro in urinary bladder (HUC-PC) cells [137].…”

Section: Toxicological Interactions Of Aflatoxins With Other Contaminantsmentioning

confidence: 96%

Aflatoxins: History, Significant Milestones, Recent Data on Their Toxicity and Ways to Mitigation

Pickova

Ostrý

Toman

et al. 2021

Toxins

114

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In the early 1960s the discovery of aflatoxins began when a total of 100,000 turkey poults died by hitherto unknown turkey “X” disease in England. The disease was associated with Brazilian groundnut meal affected by Aspergillus flavus. The toxin was named Aspergillus flavus toxin—aflatoxin. From the point of view of agriculture, aflatoxins show the utmost importance. Until now, a total of 20 aflatoxins have been described, with B1, B2, G1, and G2 aflatoxins being the most significant. Contamination by aflatoxins is a global health problem. Aflatoxins pose acutely toxic, teratogenic, immunosuppressive, carcinogenic, and teratogenic effects. Besides food insecurity and human health, aflatoxins affect humanity at different levels, such as social, economical, and political. Great emphasis is placed on aflatoxin mitigation using biocontrol methods. Thus, this review is focused on aflatoxins in terms of historical development, the principal milestones of aflatoxin research, and recent data on their toxicity and different ways of mitigation.

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“…A number of studies have reported signi cant decreases in mice body weight following exposure to a toxicant in combination to high-fat diet (HFD) or when there is co-exposure involving two toxicants [36,37]. Zhang and colleagues reported a signi cant decrease in bodyweight in mice treated with HFD plus Cd when compared with the HFD alone group and the group receiving low doses of Cd alone, intraperitoneally administered twice a week [36].…”

Section: Effects Of CD Exposure (Drinking Water) On Body Weight (G) Of Apoe (-/-) Mice For 4 Monthsmentioning

confidence: 99%

Sub-chronic cadmium exposure in drinking water dysregulates lipid and glucose metabolisms in ApoE (-/-) mice: Molecular insights into the etiology of atherosclerosis

Okpara

Wan

Huang

et al. 2021

Preprint

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Background: Exogenous exposure to cadmium is associated with cardiovascular diseases, including atherosclerosis, but experimental evidence elucidating the molecular events involved is still unclear, hence this study. We investigated whether sub-chronic cadmium exposure alone could cause a disruption in lipid and glucose metabolisms and whether the combined effect of these dysregulations could progressively lead to atherosclerosis in ApoE (-/-) mice.Methods: Experiments were performed in male ApoE (-/-) mice (8 weeks 25 ± 3g; n=60), which were randomly placed into four (4) groups according to body weight and administered cadmium (CdCl2) 0, 50, 100 and 200 mg/L, respectively in drinking water consecutively for 4 months. After treatment, changes in body weight were evaluated and mice plasma was analyzed for LDL, HDL, TCHO, TG, insulin and glucose levels. The collected samples were sent for histological examination in the 4th month and two-way ANOVA was used for statistical analysis.Results Levels of plasma LDL increased in all treatment groups but was statistically significant in the 4th month across all treatment groups when compared both with the blank control group and the first month mice. Plasma HDL levels were lowered in all treatment groups in the 2nd and 4th months when compared with the 1st month mice. Statistically significant decreases in plasma HDL levels were also observed in the 2nd month in the group receiving CdCl2 (100mg/L and 200mg/L) when compared with the blank control. TCHO andTG levels increased but was statistically significant in the former in the 1st, 2nd and 4th month at CdCl2 (100mg/L and 200mg/L) and in the latter at the same dose only in the 4th month. Moreover, fasting plasma glucose and insulin were elevated and lowered, respectively. Histological examination of aortal root also showed a dose-dependent increase in plaque formation, being most visible in the treatment group receiving CdCl2 (200mg/L).Conclusion: In line with our hypothesis, sub-chronic oral exposure of ApoE (-/-) mice to cadmium dysregulated their glucose and lipid profiles and the combined effect of this may be a contributory molecular event in the development of atherosclerosis.

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Acute oral toxicity test and assessment of combined toxicity of cadmium and aflatoxin B1 in kunming mice

Cited by 30 publications

References 42 publications

A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

Aflatoxins: History, Significant Milestones, Recent Data on Their Toxicity and Ways to Mitigation

Sub-chronic cadmium exposure in drinking water dysregulates lipid and glucose metabolisms in ApoE (-/-) mice: Molecular insights into the etiology of atherosclerosis

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