Acute phase response and hematology in pigs after cryptorchidism or inguinal hernia surgery

Abstract

Invasive surgery is a strain to the body, and many pigs undergo abdominal surgery in field practice. An observational study was performed to investigate changes in the composition of serum proteins, including acute phase proteins, and white blood cells after cryptorchidism or inguinal hernia surgery. Blood samples were collected one day before surgery and one, three, six and fourteen days after surgery. The study showed an average increase of 45.52 mg/L in C-reactive protein (CRP) day one after surgery compared to the day before surgery (p<0.0001). CRP levels were normalized on day fourteen post-surgery. On day three postsurgery, α-and β-globulins were significantly higher than before surgery. Albumin was significantly decreased on day six (p<0.01) post-surgery. On day fourteen post-surgery, total protein, α-, β-and γ-globulins were 3.45 g/L (p<0.01), 0.94 g/L (p<0.05), 1.14 g/L (p<0.001) and 2.97 g/L (p<0.0001), respectively, higher than the levels pre-surgery. There was an increase in the numbers of white blood cells from the day before surgery until the sixth day after surgery (p<0.05) and an increase in neutrophil granulocytes (NG) from the day before surgery until the day after surgery (p<0.05). The NG number stayed significantly increased throughout the test period. The average percentage amount of NG increased from 39.53 to 47.36 from the day before surgery until the third day after surgery (p<0.01), and the percentage amount of lymphocytes decreased accordingly (p<0.05). There was also an increase in the concentration of eosinophil granulocytes from the day before to the day after surgery (p<0.05). The study demonstrated how surgical procedures leads to an increase in the acute phase protein CRP, and α-, β-and γ-Glob, reduction in Alb, and changes in the WBC profile in the days following surgery.

Preparation of Porcine Allogenic Acellular Diaphragm Matrix and its Application for Umbilical Hernioplasty in Pig

“…The values of Alanine Amino Transferase, Gamma-Glutamyle transferase and Serum Albumin were remained non-significant variation during entire experiment period. In agreement with the findings of the present experiment, Munthe-Kass et al, (2018) and Nevill et al, (2010) in dog; Schreiber et al, (1982), Kumar et al, (2015) and Schulz et al, (2007) in piglet and Citil et al, (2004) and Jamma (2004) in cattle and buffaloes recorded similar results .Indirect ELISA (Table 4), absorbance at 492 nm (OD 492 )revealed negligible antigenicity of autologous Tunica Vaginalis to host tissue (Group C); however minimal extracellular matrix elicits early immunogenic and inflammatory reaction in Group A and Group B, which decreased gradually. The difference of absorbance between Group A and Group B on 15 th and 20 th day of post implantation might be due to immunosuppressive action of methylprednisolone in Group B.…”

The Effect of the Repair of Induced Articular Cartilage Defects in Pigs Using Calcium Phosphate Cement with Aminoacids on the Concentrations of Selected Inflammatory Markers and Serum Enzyme Activities

“…In addition to their importance as an indicator of disease, their main diagnostic importance is that the concentrations of APPs are correlated with the degree of tissue damage, the severity of the disease, and the rate of recovery of impaired functions after treatment [11]. Furthermore, they may be useful markers in assessing disease progression, monitoring and control of post-operative conditions, as well as in monitoring treatment efficacy [12]. From this point of view, some APPs might be useful markers for the evaluation of post-operative complications also after cartilage reconstruction, and for assessing the extent of surgical trauma.…”