Acute physical exercise alters apolipoprotein E and C-III concentrations of apo E-rich very low density lipoprotein fraction

Klein, Lavy; Miller, Todd D.; Radam, Teresa E.; O'Brien, T. G.; Nguyen, Tu T.; Kottke, Bruce A.

doi:10.1016/0021-9150(92)90049-m

Cited by 25 publications

(18 citation statements)

References 37 publications

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“…There is accumulating evidence that postprandial lipemia can be attenuated by a prior session of exercise 12) ; however, the effects of exercise performed after fat ingestion on postprandial TG concentrations are not conclusive, with some studies reporting an exercise-induced reduction and others reporting no effect [13][14][15][16][17][18] . Studies in both the fasting and postprandial state suggest that maximal exercise-induced TG attenuation occurs after a delay of some hours, rather than during or immediately after exercise.…”

Section: Discussionmentioning

confidence: 99%

“…Although it is clear that exercise performed several hours before the meal (usually on the previous day) is effective to mitigate postprandial lipemia, it is difficult for participants in the exercise to realize the effects. In the present study, we chose to have the exercise performed shortly before and after the test cream intake because the few related studies that exist all used very high fat-containing meals [13][14][15][16][17][18] . Physical activity recommendations advise that adults should engage in moderate-intensity physical activity for a minimum of 30 min on 5 or more days of the week [19][20][21] .…”

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confidence: 99%

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Acute Effects of Shortly Pre- Versus Postprandial Aerobic Exercise on Postprandial Lipoprotein Metabolism in Healthy but Sedentary Young Women

Hashimoto

Ootani

Hayashi

et al. 2011

JAT

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Acute Effects of Shortly Pre- Versus Postprandial Aerobic Exercise on Postprandial Lipoprotein Metabolism in Healthy but Sedentary Young Women

Hashimoto

Ootani

Hayashi

et al. 2011

JAT

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“…This is the first attempt to determine whether exercise can suppress the lipemic response to a meal approximating the macronutrient composition of the typical Western diet, that is (in terms of energy) 35-40% fat, about 47% carbohydrate, and about 16% protein (Williams, 2002). In fact, with the exception of Murphy et al (2000), who employed a diet providing 47% of energy from fat over the course of 1 day, all studies on the effect of exercise on postprandial lipemia have used test meals containing 60-93% of energy as fat, or 1.0-1.5 g/kg body mass (Maruhama et al, 1977;Schlierf et al, 1987;Klein et al, 1992;Aldred et al, 1994;Hardman & Aldred, 1995;Tsetsonis & Hardman, 1996a, b;Tsetsonis et al, 1997;Gill et al, 1998Gill et al, , 2001aGill et al, , b, 2002Hardman et al, 1998;Herd et al, 1998Herd et al, , 2000Herd et al, , 2001Zhang et al, 1998;Malkova et al, 1999;Gill & Hardman, 2000;Thomas et al, 2001;Petitt et al, 2003). Since the lipemic response to a fat meal is expected and has been shown to be positively related to the amount of fat ingested (Dubois et al, 1998), one could claim that exercise might not have a significant effect on lipemia after a meal of moderate fat content.…”

Section: Discussionmentioning

confidence: 99%

Effect of prior exercise on lipemia after a meal of moderate fat content

2004

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Objective: Prior exercise has been repeatedly shown to reduce lipemia after meals of exaggerated fat content (over 60% of total energy). The aim of the present study was to investigate whether the same applies to meals closer to the composition of the typical Western diet and explore whether exercise affects the release of dietary fat into the bloodstream. Design: Randomized counterbalanced. Setting: Laboratory. Subjects: Nine healthy young male volunteers. Intervention: Subjects consumed a meal of moderate fat content (35% of total energy, 0.66 g/kg body mass) 14 h after having either cycled for 1 h at 70-75% of maximal heart rate or rested. Macadamia nuts were used as the main source of dietary fat to trace its entry into the circulation because of their unusual fatty acid composition. Blood samples were drawn before the meal and for 8 h postprandially. Results: Plasma triacylglycerol concentrations and total area under the triacylglycerol concentration vs time curve (AUC) were significantly lower after exercise (P ¼ 0.001 and 0.003, respectively; effect size for the latter, 0.84). However, incremental (above baseline) AUC was not affected by exercise significantly. When controlling for differences in baseline plasma concentrations, only the fatty acids that were more abundant in the meal than in plasma triacylglycerols were decreased in the early postprandial period following exercise, implying either a suppressive effect of exercise on the rate of triacylglycerol release from the intestine or a more rapid chylomicron clearance after meal consumption. Conclusions: Exercise performed between 15 and 14 h before a meal of moderate fat content reduced postprandial lipemia, mainly by lowering fasting triacylglycerols. The effect of exercise on postprandial triacylglycerol metabolism may be mediated, at least in part, by attenuated release of dietary fat from the intestine.

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“…After 1 week, the sample was resuspended in 10 ml of 0.9% saline and injected intravenously into the same subject. We conducted additional control experiments showing that such ex vivo-labeled VLDL-TG particles are indistinguishable from native VLDL with regard to electrophoretic properties, cholesterol-to-TG ratio, apolipoprotein B-100 (apoB-100) concentrations, and mobility on size-exclusion HPLC (14). When using this procedure, z50-60% of the tracer was recovered and available for infusion.…”

Section: Ex Vivo Vldl-tg Labelingmentioning

confidence: 99%

“…A small proportion was then analyzed for TG content using a COBAS Integra 800, and plasma concentrations of VLDL-TG could then be calculated. The remaining VLDL-TG was transferred to a glass scintillation vial, 10 ml of scintillation liquid was added, and the sample was measured for 14 C activity using dualchannel counting.…”

Section: Oleate and Vldl-tg Samentioning

confidence: 99%

Measuring VLDL-triglyceride turnover in humans using ex vivo-prepared VLDL tracer

Gormsen

Jensen

Nielsén

2006

Journal of Lipid Research

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There has been more interest in VLDL-triglyceride (TG) kinetics during the last decade. Unfortunately, robust measurement methods are elaborate and not readily available. Here, we describe a method using unique, ex vivo labeling of the fatty acid moiety of VLDL-TG followed by intravenous bolus infusion in the same person. We found that plasma disappearance of ex vivo-labeled VLDL-TG was comparable to that of in vivo-labeled VLDL-TG and that turnover rates can be safely estimated from the log linear decay of VLDL-TG specific activity. We found minor labeling of the plasma FFA (oleate) pool, which was largely attributable to coinfusion of free [14 C]triolein; VLDL-TG did not contribute substantially to the plasma FFA pool. The plasma decay curve of VLDL-TG was not affected by the presence of tracer in the FFA pool, provided that the data from 2 h after the VLDL tracer bolus infusion was used. The FFA contamination problem was circumvented by minor modification of the VLDL-TG tracer preparation. The approach we describe should expand the opportunity to study processes that cannot be assessed if the FFA precursor pool is labeled. This method for VLDL-TG tracer preparation can allow measurement of VLDL turnover, tissue uptake of VLDL-TG, and oxidation of VLDL-TG.-Gormsen L. C., M. D. Jensen, and S. Nielsen. Measuring VLDLtriglyceride turnover in humans using ex vivo-prepared VLDL tracer. J. Lipid Res. 2006. 47: 99-106. Supplementary key words very low density lipoprotein . lipoproteins . free fatty acids Our present understanding of human VLDL-triglyceride (TG) kinetics and metabolism has been limited by the lack of easily available and robust methods. Although the assessment of VLDL production using splanchnic balances is thought to be accurate (1, 2), this procedure requires blood sampling from the arterial bed as well as the hepatic and portal veins in combination with measurement of splanchnic blood flow, which is impractical in human experimental settings. Traditional turnover measurement, based on isotope dilution techniques using constant infusion of labeled VLDL-TGs (3), has not been widely used in human studies because of the lack of appropriate tracers and problems with tracer recycling. Consequently, a variety of alternative approaches have been used, as recently reviewed by Magkos and Sidossis (4). Among the most widely used approaches are labeling of the TG precursor pool with FFA (1-3), glycerol (8-11), or acetate (12), which results in in vivo incorporation of the tracer into the VLDL-TG complex. Frequent blood sampling and mathematical modeling of the data are needed to calculate the plasma VLDL-TG decay. These methods do not allow one to determine the metabolic fate of the fatty acid moiety of the TG molecule and are dependent upon the validity of the mathematical model for the given experimental setting. Another approach is to collect and isolate in vivo-labeled VLDL particles and reinfuse them into the same subject at a later time (13). However, using this approach, only a small amount ...

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Acute physical exercise alters apolipoprotein E and C-III concentrations of apo E-rich very low density lipoprotein fraction

Cited by 25 publications

References 37 publications

Acute Effects of Shortly Pre- Versus Postprandial Aerobic Exercise on Postprandial Lipoprotein Metabolism in Healthy but Sedentary Young Women

Acute Effects of Shortly Pre- Versus Postprandial Aerobic Exercise on Postprandial Lipoprotein Metabolism in Healthy but Sedentary Young Women

Effect of prior exercise on lipemia after a meal of moderate fat content

Measuring VLDL-triglyceride turnover in humans using ex vivo-prepared VLDL tracer

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