Acute porphyria. I. some observations on the solubility, precipitation, fluorescense and absorbtion spectra of calcium phosphate-adsorbed porphyrin pigments

Eriksen, L.

doi:10.3109/00365515109060584

Cited by 6 publications

(1 citation statement)

References 5 publications

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“…) The main portion of the pigment passed through the column, preceded by a blue fluorescent band which was found to be due to fat. At the top of the column, a dark brown pigment remained which was eluted later with chloroform/acetic acid, and gave a greenish fluorescence and two-banded very indistinct spectrum (Eriksen, 1951).…”

Section: Purificationmentioning

confidence: 99%

The biology of Asterias rubens L.V. A Porphyrin pigment in the integument

Kennedy

Vevers

1953

J. Mar. Biol. Ass.

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A porphyrin was extracted by MacMunn (1886), working at Plymouth, from the integument of Asterias rubens then known as Uraster rubens. He extracted whole starfishes with ethanol and ammonia and also with ethanol and sulphuric acid. After diluting his acid-ethanol solution with water he shook the extract with chloroform; the chloroform solution showed two absorption bands, at 607-593 and 566-548 mfl-. On evaporation this extract yielded a brown amorphous residue which he found to be soluble in absolute ethanol giving a red solution with the same absorption spectrum. On adding ammonia four absorption bands appeared, at 632-622, 586-566, 548-529 and 516-490 mfl-. He considered that this and similar pigments from the slug Arion empiricorum, and the coelenterates Flabellum variabile and Fungia symmetrica were identical with the haematoporphyrin of Hoppe-Seyler (1871) which was then the only porphyrin known. Experimental work has now shown that in other animals examined and found to contain porphyrin this pigment is never present in the form of haematoporphyrin. A reinvestigation has therefore been made of the occurrence of porphyrin in the starfish, Asterias rubens.Certain details of the distribution in #le Plymouth area of different colour .forms of A. rubens have already been recorded (Vevers, 1949). In general, starfishes on the Rame-Eddystone grounds are dark brown or red-brown, but specimens coloured violet, violet-brown, red or even pale pink are not uncommon. In Plymouth Sound, on the other hand, 'a pure population of bright red starfishes was found in 1948; this population has now been greatly reduced in number but specimens can still be obtained.In the present work four different colour types were used. The choice of these types was arbitrary, but they were nearly all colours which could be easily recognized in a sample of this species. The colour types used were dark brown, violet-brown and pale, all from the Rame-Eddystone grounds, and red (bright red) from the Plymouth Sound population. The violet-brown specimens were very close to the val'. violacea of earlier writers.

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Section: Purificationmentioning

confidence: 99%