Acute psychoactive and toxic effects of D. metel on mice explained by 1H NMR based metabolomics approach

Fu, Yong-Hong; Si, Zhi-Hong; Li, Pu-Min; Li, Minghui; Zhao, He; Jiang, Lei; Xing, Yue-Xiao; Wei, Hong; Ruan, Ling-Yu; Wang, Junsong

doi:10.1007/s11011-017-0038-9

Cited by 12 publications

(2 citation statements)

References 41 publications

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“…This increase in oxidative stress could result from an increase in peroxide accumulation in the brains of DM mice leading to the formation of ROS and disruption in the activities of CAT and GPx, the principal antioxidant defence enzymes that eliminate intracellular peroxides. This assertion is supported by previous studies which showed catalase and GSH depletion in cells having increased levels of H2O2 following oxidative damage [ 55 , 56 ].…”

Section: Discussionsupporting

confidence: 84%

Datura metel stramonium exacerbates behavioral deficits, medial prefrontal cortex, and hippocampal neurotoxicity in mice via redox imbalance

Igben,

Iju,

Itivere

et al. 2023

Lab Anim Res

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Background Datura metel (DM) stramonium is a medicinal plant often abused by Nigerians due to its psychostimulatory properties. Hallucinations, confusion, agitation, aggressiveness, anxiety, and restlessness are reported amongst DM users. Earlier studies suggest that DM induces neurotoxicity and affect brain physiology. However, the exact neurological effects of DM extract in the medial prefrontal cortex (mPFC) and hippocampal morphology have not been elucidated. In this study, we evaluated the hypothesis that oral exposure to DM extract exerts a neurotoxic effect by increasing oxidative stress in the mPFC and the hippocampus and induces behavioral deficits in mice. Results DM methanolic extract exposure significantly increased MDA and NO levels and reduced SOD, GSH, GPx and CAT activities in mice brains. In addition, our results showed that DM exposure produced cognitive deficits, anxiety, and depressive-like behaviour in mice following oral exposure for 28 days. Moreover, the mPFC and hippocampus showed neurodegenerative features, loss of dendritic and axonal arborization, a dose-dependent decrease in neuronal cell bodies’ length, width, area, and perimeter, and a dose-dependent increase in the distance between neuronal cell bodies. Conclusions Oral exposure to DM in mice induces behavioural deficits, mPFC and hippocampal neuronal degenerations via redox imbalance in the brain of mice. These observations confirm the neurotoxicity of DM extracts and raises concerns on the safety and potential adverse effects of DM in humans. Graphical abstract

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Section: Discussionsupporting

confidence: 84%

Datura metel stramonium exacerbates behavioral deficits, medial prefrontal cortex, and hippocampal neurotoxicity in mice via redox imbalance

Igben,

Iju,

Itivere

et al. 2023

Lab Anim Res

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“…In addition, a heavy load of ROS could enhance peroxidation of membrane lipids, leading to damage the cellular membranes [26]. The decreased level of choline and ethanolamine were seen in the D group compared with the CK group, and both are considered as the key players in the stability and integrity of cell membranes [27]. Therefore, any decrease in choline and ethanolamine may be due to excessive utilization for repairing the damaged membranes caused by ROS which are indicators of decreased level of sarcosine in D group [28].…”

Section: Discussionmentioning

confidence: 99%

The Antifungal Potential of Carvacrol against Penicillium Digitatum through 1H-NMR Based Metabolomics Approach

et al. 2019

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Carvacrol (5-Isopropyl-2-methylphenol), a volatile oil constituent, mainly exists in Labiaceae family plants. Carvacrol has long been studied for its natural antifungal potential and food preservative potential. However, its exact mode of action, especially against Penicillium digitatum (P. digitatum), remains unexplored. Herein, a 1 H-NMR-based metabolomic technique was used to investigate the antifungal mechanism of carvacrol against P. digitatum. The metabolomic profiling data showed that alanine, aspartate, glutamate, and glutathione metabolism were imbalanced in the fungal hyphae. A strong positive correlation was seen between aspartate, glutamate, alanine, and glutamine, with a negative correlation among glutathione and lactate. These metabolic changes revealed that carvacrol-induced oxidative stress had disturbed the energy production and amino acid metabolism of P. digitatum. The current study will improve the understanding of the metabolic changes posed by plant-based fungicides in order to control citrus fruit green mold caused by P. digitatum. Moreover, the study will provide a certain experimental and theoretical basis for the development of novel citrus fruit preservatives.aroused by excessive use of certain routine and synthetic antifungal agents. Over the past few decades, the intentions have been to ensure the hygiene and safety levels of fruit products along with promoting maximum biodegradability. In order to achieve this ambition, researchers are still trying to screen natural products with strong antifungal potential from plants. Moreover, it has also been tried to explore new preservation technologies that can replace synthetic fungicides. Compared with synthetic fungicides, natural antifungal substances have attained much attention in recent years [6][7][8][9].Various essential oils refined from Melaleuca alternifolia have strong inhibitory effects on multiple fungal strains, particularly P. italicum Wehmer and P. digitatum Sacc., and hence can be used as natural preservatives in agricultural and food processing [10]. Previously we have reported that cinnamaldehyde contained in cinnamon has a good antifungal effect against P. italicum and clarifies the method for dynamic detection of the antifungal effect of cinnamaldehyde [11]. In addition, GC-MS and GC-FID techniques successfully separated the essential oils components viz. limonene (87.02%), linalyl acetate (53.76%) and linalool (39.74%) from peels, leaves and flowers of Citrus aurantium var amara, neroli oil appeared as the best fungistatic agent, which reduced the P. digitatum sporulation to 25 % at a 50 mg/mL concentration of oil [12].Carvacrol (5-Isopropyl-2-methylphenol) is the major volatile oil constituent from Labiaceae family plants, such as oregano, mint, and thyme [13]. Carvacrol has many medicinal and edible applications, including antifungal, antimicrobial, anti-inflammatory, insecticidal, antitumor, and other effects [14]. Carvacrol inhibited the growth of P. digitatum and P. italicum in in vitro and in vivo experiments of l...

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Neurotoxic Potential of Alkaloids from Thorn Apple (Datura stramonium L.): A Commonly Used Indian Folk Medicinal Herb

Choudhary

Sharma

Agrawal

et al. 2021

Medicinal Herbs and Fungi

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Acute psychoactive and toxic effects of D. metel on mice explained by 1H NMR based metabolomics approach

Cited by 12 publications

References 41 publications

Datura metel stramonium exacerbates behavioral deficits, medial prefrontal cortex, and hippocampal neurotoxicity in mice via redox imbalance

Datura metel stramonium exacerbates behavioral deficits, medial prefrontal cortex, and hippocampal neurotoxicity in mice via redox imbalance

The Antifungal Potential of Carvacrol against Penicillium Digitatum through 1H-NMR Based Metabolomics Approach

Neurotoxic Potential of Alkaloids from Thorn Apple (Datura stramonium L.): A Commonly Used Indian Folk Medicinal Herb

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