Acute reduction of serum 8-iso-PGF2-alpha and advanced oxidation protein products in vivo by a polyphenol-rich beverage; a pilot clinical study with phytochemical and in vitro antioxidant characterization

Abstract

BackgroundMeasuring the effects of the acute intake of natural products on human biomarker concentrations, such as those related to oxidation and inflammation, can be an advantageous strategy for early clinical research on an ingredient or product.Methods31 total healthy subjects were randomized in a double-blinded, placebo-controlled, acute pilot study with post-hoc subgroup analysis on 20 of the subjects. The study examined the effects of a single dose of a polyphenol-rich beverage (PRB), commercially marketed as "SoZo®", on serum anti-inflammatory and antioxidant markers. In addition, phytochemical analyses of PRB, and in vitro antioxidant capacity were also performed.ResultsAt 1 hour post-intake, serum values for 8-iso-PGF2-alpha and advanced oxidation protein products decreased significantly by 40% and 39%, respectively. Additionally, there was a trend toward decreased C-reactive protein, and increased nitric oxide levels. Both placebo and PRB treatment resulted in statistically significant increases in hydroxyl radical antioxidant capacity (HORAC) compared to baseline; PRB showed a higher percent change (55-75% versus 23-74% in placebo group), but the two groups did not differ significantly from each other.ConclusionsPRB produced statistically significant changes in several blood biomarkers related to antioxidant/anti-inflammatory effects. Future studies are justified to verify results and test for cumulative effects of repeated intakes of PRB. The study demonstrates the potential utility of acute biomarker measurements for evaluating antioxidant/anti-inflammatory effects of natural products.

Effect of Modest Caloric Restriction on Oxidative Stress in Women, a Randomized Trial

“…However, in the study on effect of diet-induced reduction in oxidative stress it has been showed that F 2 -isoprostane level was well correlated with advanced oxidation protein products [69]. In addition, one should be cautious if generalizing this study’s finding to males, obese individuals, and populations with serious obesity-related diseases, such as type 2 diabetes or hyperlipidemias.…”

Effects of red orange juice intake on endothelial function and inflammatory markers in adult subjects with increased cardiovascular risk

“…A reduction of oxidative stress biomarkers such as 8-isoprostaglandin F2a has been reported after consumption of blonde orange juice (44) or other polyphenol-rich beverages (45). Free radicals and reactive oxygen and nitrogen species may react with proteins and lead to the conversion of amino acid residues into carbonyl groups.…”

Biologically Active Compounds from Hops and Prospects for Their Use

“…Through numerous animal and clinical experiments it has been assumed that most of the beneficial effects of polyphenols are related to their antioxidant properties. Polyphenols have been shown to be effective antioxidants in a wide range of chemical oxidation systems, as demonstrated by their ability to scavenge reactive oxygen (ROS) or nitrogen species such as hydroxyl, peroxyl, superoxide, hydrogen peroxide, or singlet oxygen (Ross and Kasum ; Nemzer and others ; Sandoval‐Acuna and others ). These radicals are involved in oxidative stress and may play crucial roles in the development of tumors, atherosclerosis, diabetes, Alzheimer's disease, Parkinson's disease, or in the entire ageing process (Mladenka and others ; Nemzer and others ).…”

“…However, excessive inflammation can be dangerous and may lead to pathological changes in cells. Inflammatory abnormalities are a large group of disorders that are associated with numerous diseases such as cancer, atherosclerosis, erectile dysfunction, arthritis, obesity, or ischemic heart disease (Nemzer and others ; Raza and others ). In a significant number of studies, in vitro and in vivo , the effect of polyphenols on expression and activity of pro‐inflammatory factors have been demonstrated.…”

“…Nitric oxide is a ubiquitous compound involved in inflammation and plays a crucial role in vasodilation via the relaxation of vascular smooth muscles (Nemzer and others ). Excessive production of NO is catalyzed by iNOS (González‐Gallego and others ).…”