Acute salinity stress alters the haemolymph metabolic profile of Penaeus monodon and reduces immunocompetence to white spot syndrome virus infection

Joseph, Annies; Philip, Rosamma

doi:10.1016/j.aquaculture.2007.08.047

Cited by 93 publications

(50 citation statements)

References 39 publications

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“…Many studies in crustaceans have proven the inference (Luana et al 2007;Visudtiphole et al 2010;Duan et al 2014), that the expression of CRTs was up-regulated in hepatopancreas after temperature and pathogen challenges. Interestingly, the expression of CRT was suppressed after salinity challenges in this study, which suggests that the stress and immune response functions of CRT were disturbed in salinity stress (Joseph and Philip 2007). This inference is consistent with the phenomenon of increased mortality and decreased immunity caused by salinity changes in the crustaceans (Joseph and Philip 2007;Romano and Zeng 2006;Prangnell and Fotedar 2006); however, the specific mechanism needs further verification.…”

Section: Discussionsupporting

confidence: 74%

“…Interestingly, the expression of CRT was suppressed after salinity challenges in this study, which suggests that the stress and immune response functions of CRT were disturbed in salinity stress (Joseph and Philip 2007). This inference is consistent with the phenomenon of increased mortality and decreased immunity caused by salinity changes in the crustaceans (Joseph and Philip 2007;Romano and Zeng 2006;Prangnell and Fotedar 2006); however, the specific mechanism needs further verification. Osmotic and ionic regulation in the crustacea is mostly accomplished by the multifunctional gills.…”

Section: Discussionsupporting

confidence: 74%

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Cloning and characterization of calreticulin and its association with salinity stress in P. trituberculatus

Wang

Zhang

et al. 2015

Cell Stress and Chaperones

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Calreticulin (CRT) is a highly conserved and multifunctional endoplasmic reticulum (ER) chaperone protein and plays important roles in salinity stress response. Portunus trituberculatus is a commercially important fishery species, and water salinity conditions influence its commercial farming significantly. In order to research the function of calreticulin under salinity stress, the full-length cDNA sequence of calreticulin from P. trituberculatus (PtCRT) was firstly cloned and characterized. The complete cDNA sequence of PtCRT is 1676 bp with 1218 bp open reading frame (ORF), encoding a polypeptide of 405 amino acids. Multiple sequence alignments showed that the deduced acid amino sequences of PtCRT shared the highest homology to CRT of Fenneropenaeus chinensis (89 %). Fluorescent quantitative real-time PCR analysis indicated that PtCRT was expressed in all detected tissues and showed the highest expression level in hepatopancreas. In addition, salinity challenge significantly influenced the expression level of PtCRT in gill. Six single nucleotide polymorphisms (SNPs) were detected in cDNA sequence of PtCRT, and one SNP was associated with the salt tolerant trait. All results indicated that PtCRT plays an important role in mediating the salinity adaption of P. trituberculatus.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionsupporting

confidence: 74%

Cloning and characterization of calreticulin and its association with salinity stress in P. trituberculatus

Wang

Zhang

et al. 2015

Cell Stress and Chaperones

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“…Liu, Hou, Wang, & Liu, 2015). Despite of previous reports that the innate immunity of L.vannamei was depressed at low salinity (Joseph & Philip, 2007;C.-C. Li, Yeh, & Chen, 2010).Five up-regulated immune-related genes were discovered in the hepatopancreas SSH library, including kazal-type proteinase inhibitor vanpin-2, chitinase, glutathione S-transferase, MnSOD and lectin A isoform 1. The up-regulation of these genes indicated that shrimps may keep homeostasis of internal environment www.ccsenet.org/ijb…”

Section: Cell Defense Homeostasis and Immunementioning

confidence: 83%

Profiling of Up-Regulated Genes Response to Acute Hypo-Osmotic Stress in Hepatopancreas and Gill of the Pacific White Shrimps (Litopenaeus vannamei)

Liu¹,

Sun²,

Dong³

et al. 2016

IJB

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Suppression subtractive hybridization (SSH) was applied to screen responsively up-regulation genes in hepatopancreas and gill of Litopenaeus vannamei induced by acute hypo-osmotic stress. In the hepatopancreas, 196 clones were randomly selected and sequenced. 131 non-redundant transcripts, corresponding to 41 genes, were found with elevated expressions. They were functionally clustered into eight biological processes which were protein synthesis and processing, carbohydrate metabolism and energy production, transport, cell growth, apoptosis, cytoskeletal component, cell defense and homeostasis, signal transduction, accounting for 26.2%, 14.3%, 16.7%, 4.7%, 4.7%, 2.3%, 11.9% and 7.1%, respectively. When confirmed by real time qRT-PCR, the gene expression levels of MnSOD, glutathione and glutamine synthetase showed significant increases (2.64-folds, 3.44-folds and 2.16-folds, respectively) in hepatopancreas (P<0.05). Totally 81 expressed sequence tags (ESTs) were obtained by random sequencing from the gill SSH cDNA library, and 52 unigenes, including 15 contigs and 37 singlets, after CP3 online matching were gained by clustering and assembling. The sequence alignment results revealed that 30 of them had significant homology to previously identified Genbank database sequences, while 22 unigenes did not match any sequence in the datebase and presumably represented unidentified cDNAs. Functionally, the unigenes were classified into six clusters, including ion channels and transport proteins (1); protein synthesis, translation and transcription factor (2); stress resistance and antioxidant factors (3); energy metabolism (4); signal receptor and transduction (5); cell fibrin and cytoskeletal proteins (6) with proportion of 23.3%, 20.0%, 20.0%, 16.7%, 10.0% and 10.0% respectively. Real time qRT-PCR confirmation revealed significant elevation of Arginine kinase, Carbonic anhydrase and NaK-ATPase-α-subunit expressions (3.73-folds, 2.55-folds and 5.83-folds, respectively) in the gill after acute low-osmotic stress (P < 0.05). These results provided insight into critical physiology processes and pathways constituting the hypo-osmotic stress adaption program in hepatopancreas and gill of L. vannamei.

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“…For some viruses, such as white spot syndrome virus, disease outbreaks appear to result from the amplification of viral loads in response to environmental stimuli such as stress or shifts in temperature or salinity (Joseph & Philip, 2007;Peng et al, 1998;Rahman et al, 2006;Vidal et al, 2001). For other viruses, the prevalence of persistent infections may be very high without any recorded evidence of disease (Sittidilokratna et al, 2009b;Wijegoonawardane et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of gill-associated virus and Mourilyan virus during mixed infections of black tiger shrimp (Penaeus monodon)

Oanh

Hulten

Cowley

et al. 2011

Journal of General Virology

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Acute salinity stress alters the haemolymph metabolic profile of Penaeus monodon and reduces immunocompetence to white spot syndrome virus infection

Cited by 93 publications

References 39 publications

Cloning and characterization of calreticulin and its association with salinity stress in P. trituberculatus

Cloning and characterization of calreticulin and its association with salinity stress in P. trituberculatus

Profiling of Up-Regulated Genes Response to Acute Hypo-Osmotic Stress in Hepatopancreas and Gill of the Pacific White Shrimps (Litopenaeus vannamei)

Pathogenicity of gill-associated virus and Mourilyan virus during mixed infections of black tiger shrimp (Penaeus monodon)

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