Acute stress response in triploid rainbow trout (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis)

Benfey, Tillmann J.; Biron, Michel

doi:10.1016/s0044-8486(99)00314-2

Cited by 132 publications

(100 citation statements)

References 29 publications

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“…The increase of catecholamines concentration may result in erythrocytes release to the blood stream by spleen contraction (McDonald & Milligan, 1997). Increase of parameters involving red blood cells, such as hemoglobin and hematocrit, has been observed in response to stress (Benfey & Biron, 2000;Wojtaszek et al, 2002;Urbinati et al, 2004). However, vitamin C did not affect hematocrit of matrinxã after air exposure in this study, differing with the findings by Barros et al (2002) in tilapia (Oreochromis niloticus), whose elevation of hematocrit was directly related to the dietary vitamin C levels.…”

contrasting

confidence: 95%

“…The hyperglycemia reported in several teleosts during stress is primarily mediated by catecholamines and lately by cortisol (Wendelaar Bonga, 1997). Increase in blood glucose and cortisol is reported in several studies (Benfey & Biron, 2000;Sadler et al, 2000;Urbinati et al, 2004) and, similarly to the cortisol response, excepting for T100 fish, vitamin C did not interfere in the blood glucose response of fish in this study. However, the glucose values found after air exposure were more elevated than the pre-stress values described by Urbinati et al (2004) (44 mg.dl -1 ) in juveniles and by Carneiro & Urbinati (2002) (40mg.dl -1 ) in adults of the same species.…”

supporting

confidence: 87%

“…Decrease in lymphocyte and increase in neutrophil number are frequently reported in stressed fish (Tort et al, 1996;Rotlant et al, 1997;Benfey & Biron, 2000;Iida & Kurogi, 2001). No significant differences were observed in the total number of leukocyte of matrinxã along the samplings and the values obtained (4,9 to 5,8 cell μL -1 x 10 3 ) were higher than those described by Tavares-Dias et al (1999) for the species (2,6 cell μL -1 x 10 3 ).…”

mentioning

confidence: 97%

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Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Abreu

Urbinati

2006

Acta Amaz.

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The role of vitamin C on physiological responses of matrinxã (Brycon amazonicus) submitted to air exposure was analyzed. Nine hundred fish (70.15 g) were distributed in fifteen 500 l boxes (60 fish.box -1 ) and fed five rations (treatments): Control (no vitamin C); T100 (100 mg); T200 (200 mg); T400 (400 mg) and T800 (800 mg of vitamin C kg.ration -1 ). Each ration was offered to fish of three boxes during 60 days before the stress challenge that consisted of exposing fish to air for two minutes. Samplings were carried out for 5, 15, 30 and 60 minutes after the air exposure. Blood was collected for glucose, cortisol, total protein, sodium, chloride, hematocrit, hemoglobin determination, and white and red cell count. Liver was removed for hepatosomatic index (HSI) calculation and glycogen determination. Vitamin C did not affect the levels of cortisol, chloride, total protein, hemoglobin, leukocytes, hepatic glycogen or HSI in air exposed fish. Blood glucose levels elevation observed 60 minutes after the challenge did not depend on the levels of vitamin C, nor did the drop in serum sodium levels verified 60 minutes after stressor. In general, hematocrit did not change by effect of vitamin C but it was lower at 15 and 30 minutes after the challenge. The number of erythrocytes decreased in fish after 5 minute sampling in all treatments, especially at 30 and 60 minutes. The air exposure evoked alterations in stress indicators of matrinxã, and the vitamin C did not alter the responses. KEY WORDSBrycon amazonicus, capture, stress, ascorbyl -polyphosphate. de glicose, cortisol, proteína total, sódio, cloreto, hematócrito, hemoglobina, contagem Respostas fisiológicas de matrinxã (Brycon amazonicus) arraçoados com diferentes níveis de vitamina C e submetidos à exposição aérea RESUMO Este trabalho avaliou a participação da vitamina C nos indicadoras de estresse no matrinxã (Brycon amazonicus), durante exposição aérea. Novecentos peixes (70,15g) foram distribuídos em caixas de cimento (500 l) e alimentados com os tratamentos: Controle (sem vitamina C); T100 (100 mg); T200 (200 mg); T400 (400 mg); T800 (800 mg de vitamina C kg.ração -1 ). Cada ração foi fornecida a peixes de três caixas por 60 dias antes da aplicação do estressor, que consistiu em suspender os peixes mantendo-os no ar por dois minutos. Cinco, 15, 30 e 60 minutos depois, os peixes anestesiados foram amostrados. Foi coletado sangue para determinação PALAVRAS -CHAVEBrycon amazonicus, captura, estresse, ascorbil polifosfato.

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contrasting

confidence: 95%

supporting

confidence: 87%

mentioning

confidence: 97%

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Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Abreu

Urbinati

2006

Acta Amaz.

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“…Tort et al (1994) [45] and Altimiras et al (1994) [46] reported a decrease of haematocrit value, in response to hypoosmotic shock, in sea bream, Sparus aurata. On the other hand, Benfey & Biron (2000) [47] showed that acute stress causes an elevation in haematocrit value, in contrast with above results. Moreover, Mazur & Iwama (1993) [48] reported no changes in haematocrit contents in chinook salmon subjected to handling stress.…”

Section: Discussioncontrasting

confidence: 54%

Haematological and Immunological Responses in Juvenile Sea Bass (Dicentrarchus labrax L.) After Short-Term Acute Stress

Maricchiolo¹,

Caruso²,

Genovese³

2008

TOFISHSJ

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Abstract:The physiological effects of acute stress (induced by an intraperitoneal injection) on the haematocrit, haemoglobin, serum cortisol and glucose, haemagglutinins and lysozyme were investigated in juvenile sea bass Dicentrarchus labrax. A total of 40 fish were subjected to an intraperitoneal injection. Eight fish were sampled at 0, 2, 4, 24 and 48 h after acute stress and compared to unstressed fish (controls) sacrificed at the same times. Intraperitoneal injection resulted in significant changes in cortisol and glucose 4 and 2 hours after exposure to stress, respectively, but not in haematocrit and haemoglobin levels. No significant differences between stressed and control fish were recorded for lysozyme content and haemagglutinating activity throughout the experiment. In juvenile sea bass acute stress caused by intraperitoneal injection was mostly associated with the increase in common stress indicators (cortisol and glucose), while no clear response in terms of non-specific immune parameters was evidenced.

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“…A decrease in thrombocyte number and WBC in triploid jundia observed in this work corroborated the findings of Budiño et al (2006) and Benfey and Biron (2000). The induction of triploidy in fish has usually been reported to be accompanied by modification in physiology and reduced resistance to acute stress (Cotter et al, 2000).…”

Section: Discussionsupporting

confidence: 90%

Triploidy in the hematology of jundia juveniles (Siluriformes: Heptapteridae)

et al. 2012

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This study compared the hematological characteristics of diploid and triploid of jundia, Rhamdia quelen juveniles, an important freshwater fish cultured in south Brazil. Hematological morphometry of erythrocytes were determined in blood smears under a light microscope. The blood was used to measure the number of red blood cells (RBC) with a hemocytometer Neubauer chamber, and the numbers of white blood cells (WBC) and thrombocytes that were obtained using an indirect method. The results showed that triploidy increased (p < 0.01) the size and volume of the erythrocytes. Nevertheless, as expected, triploidy decreased (p < 0.01) the number of circulating erythrocytes, leucocytes and trombocytes in the blood of jundia. Moreover differential cell counts were different in diploids and triploids, suggesting that triploidy affects the number of cells and their proportion in blood. Lymphocytes were the most predominant cells in the differential counting of diploid fish (62.5%) while monocytes were predominant in triploid fish (49.6%). These results suggest performance differences between ploidies of jundia, and require future studies to evaluate the potential of triploid jundia in the culture conditions and resistance to infection.Keywords: Rhamdia quelen, triploid, diploid, blood cells. Triploidia na hematologia de juvenis de jundiá (Siluriformes: Heptapteridae)Resumo O presente estudo comparou características hematológicas de juvenis de jundiás Rhamdia quelen diploides e triploides, um importante peixe de água doce cultivado no sul do Brasil. A morfometria hematológica dos eritrócitos foi determinada em extensões sanguíneas sob microscopia óptica comum. O sangue dos animais foi também utilizado para mensurar o número total de eritrócitos em câmera de Neubauer, bem como os números totais de leucócitos e trombócitos pelo método indireto. Os resultados demonstraram que a triploidia aumentou (p < 0,01) o tamanho e volume dos eritrócitos. No entanto, como esperado, a triploidia reduziu (p < 0,01) o número de eritrócitos, leucócitos e trombócitos circulantes do jundiá. Além disto, a contagem diferencial de células sanguíneas foi diferente em diploides e triploides, sugerindo que a triploidia afeta o número das células, bem como sua proporção no sangue. Linfócitos foram as células predominantes na contagem de leucócitos de peixes diploides (62,5%), enquanto os monócitos foram os predominantes nos peixes triploides (49,6%). Estes resultados sugerem desempenhos diferentes entre as ploidias do jundiá e exige estudos futuros para avaliar o potencial dos jundiás triploides sob condições de cultivo e frente à infecção.Palavras-chave: Rhamdia quelen, triploide, diploide, células sanguíneas.

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Acute stress response in triploid rainbow trout (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis)

Cited by 132 publications

References 29 publications

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Haematological and Immunological Responses in Juvenile Sea Bass (Dicentrarchus labrax L.) After Short-Term Acute Stress

Triploidy in the hematology of jundia juveniles (Siluriformes: Heptapteridae)

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