Acute toxic effects of 8‐epidiosbulbin E, a 19‐norclerodane diterpene from yam Dioscorea antaly, on medaka Oryzias latipes embryos

Abstract

The fractionation of an aqueous extract of yam Dioscorea antaly from Madagascar led to the isolation of terpenoids and flavonoids. Compounds were identified on the basis of modern mass spectrometry and two-dimensional nuclear magnetic resonance (2D-NMR). Toxicological effects of the most abundant isolated compound, 8-epidiosbulbin E were studied on medaka Oryzias latipes embryo-larval development. The lethal concentration (killing 50%; LC(50) ) to embryos treated 24 h before hatching and for 3 days after hatching was estimated to be 0·56 mg ml(-1) (P< 0·05). No mortality was observed with O. latipes larvae exposed after hatching until day 4. Anatomo-pathological studies of embryos exposed to 0·56 mg ml(-1) showed development anomalies of the central nervous system, liver, muscle and intestine. The present data thus extend the model of O. latipes embryos as a useful animal model to analyse the effects of food toxins.

Bioactive Micronutrients in Coffee: Recent Analytical Approaches for Characterization and Quantification

“…Antimicrobial activities are found in a recent review [81]. Some diterpenes are toxic to insects [82] and to embryos of medaka, Japanese rice fish [83]. Structure of cafestol is given in Figure 3.…”

Marked toxicity of Albizia bernieri extracts on embryo–larval development in the medaka fish (Oryzias latipes)

“…In this case in addition widening of the sinusoidal capillaries was clearly observed in the liver and the kidney was disturbed (Rakotobe et al, 2010a). Finally, a 19-norclerodane diterpene, 8-epidiosbulbin E, isolated from D. antaly tested on the same animal model (Rakotobe et al, 2010b), displays a LC 50 of 0.56 mg mL À1 , which is also high compared to the present study. It should be noted that convulsive alkaloids have been isolated from Dioscorea dumetorum (David and Michael, 1985).…”