Acute toxic effects of polyethylene microplastic on adult zebrafish

Mak, Chu Wa; Yeung, Kirsten; Chan, King Ming

doi:10.1016/j.ecoenv.2019.109442

Cited by 202 publications

(62 citation statements)

References 96 publications

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“…Bent tails have also been reported in microplastic-treated Danio rerio adults at moderate and high concentrations [104], coinciding with another article as well [105]. In the former study, bent tails observed in polyethylene microplastic treated Danio rerio may be associated with "knockdown of the cysteine-rich motor neuron 1 gene (crim1) or missense mutation in polycystin-2(pkd2)."…”

Section: Hatchingsupporting

confidence: 76%

Embryotoxic and teratogenic effects of polyethylene microbeads found in facial wash products in Zebrafish (Danio rerio)using the Fish Embryo Acute Toxicity Test

Guzman

Chua

Sedano

2020

Preprint

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Use of polyethylene beads in facial cleansers has been continuously questioned by scientific communities for they adversely affect aquatic organisms once these beads find their way into their habitats. This study specifically aims to determine Danio rerio mortality rate using lethal endpoints and to evaluate sublethal teratogenic effects in Danio rerio due to polyethylene microbead exposure. Danio rerio, a model organism for ecotoxicology, was subjected to the Fish Embryo Acute Toxicity Test. Embryos were exposed to polyethylene microbead suspensions (PE-MBS) of varying concentrations (i.e., 20 μg/L, 200 μg/L, 2000 μg/L). They were also exposed to 5% ethanol (positive control), reconstituted water (negative control), 0.01% Tween 80 (emulsifier control), and 1% DMSO (solvent control). Toxicological endpoints (i.e., egg coagulation, lack of somite formation, non-detachment of tail, and lack of heartbeat) were observed every 24 hours until the 96th hour exposure. Hatching was observed from 48 hpf while teratogenicity was observed at 144 hpf. Significant differences between means and variances were observed for all treatment groups in relation to the negative control. For all groups, 0.01% Tween 80, 1% DMSO and 20 μg/L PE-MBS did not significantly differ with the negative control due to negligible concentration but 5% ethanol and higher concentrations of PE-MBS did. This indicated that high concentrations of PE-MBS exposure may induce early hatching, mortality, increased malformation, and increased heart rate. Tukey Kramer post hoc Test substantiated that PE-MBS toxicity is dose dependent since embryotoxicity and teratogenicity increases at higher concentrations. LC50 obtained using probit analysis based on experimental data was 2455.096 μg/L, and was higher than the concentrations used in this study. Further studies should be conducted to know more about the adverse effects of polyethylene microbeads to the biota.Author SummaryMargaret De Guzman, MSc, Patricia Chua, and Franceska Sedano have all equally contributed to this work in conceptualization, formal analysis, funding acquisition, and investigation. All authors have also equally headed project administration, procurement of resources and writing.

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Section: Hatchingsupporting

confidence: 76%

Embryotoxic and teratogenic effects of polyethylene microbeads found in facial wash products in Zebrafish (Danio rerio)using the Fish Embryo Acute Toxicity Test

Guzman

Chua

Sedano

2020

Preprint

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“…Although MP encounter by fish is assumed to occur mainly during active feeding, there is increasing evidence of MPs being encountered via the gills and/or epidermis of wild freshwater fish (Abbasi et al, 2018;Hurt et al, 2020;Park et al, 2020). Experimental studies have also demonstrated MP accumulation on the gills (Mak et al, 2019;Roch et al, 2020). Passive uptake of MPs is thus an additional source of MPs following environmental exposure during swimming and respiration.…”

Section: Mp Encounter Rates In Freshwater Fishesmentioning

confidence: 99%

“…In general, much of the experimental MP literature for freshwater fish has focussed on using various life stages of cyprinids (Table 2), especially zebrafish, as the model species to test the effects of MP exposure, most likely due to their extensive use in toxicology research (Dai et al, 2014). The broad diet and different feeding behaviours of zebrafish (Froese & Pauly, 2019) allow several MP exposure methods, including presented paired with food items (Batel et al, 2018;Lei et al, 2018;Lu et al, 2016), within feed (Mak et al, 2019) and/or via contaminated prey resources (Batel et al, 2016(Batel et al, , 2020.…”

Section: Impac Ts Of Ing E S Ted Mps On Fre S Hwater Fis He Smentioning

confidence: 99%

Microplastics in freshwater fishes: Occurrence, impacts and future perspectives

et al. 2021

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Microplastics (MPs) are small, plastic particles of various shapes, sizes and polymers.Although well studied in marine systems, their roles and importance in freshwater environments remain uncertain. Nevertheless, the restricted ranges and variable traits of freshwater fishes result in their communities being important receptors and strong bioindicators of MP pollution. Here, the current knowledge on MPs in freshwater fishes is synthesized, along with the development of recommendations for future research and sample processing. MPs are commonly ingested and passively taken up by numerous freshwater fishes, with ingestion patterns often related to individual traits (e.g. body size, trophic level) and environmental factors (e.g. local urbanization, habitat features). Controlled MP exposure studies highlight various effects on fish physiology, biochemistry and behaviour that are often complex, unpredictable, species-specific and nonlinear in respect of dose-response relationships.Egestion is typically rapid and effective, although particles of a particular shape and/ or size may remain, or translocate across the intestinal wall to other organs via the blood. Regarding future studies, there is a need to understand the interactions of MP pollution with other anthropogenic stressors (e.g. warming, eutrophication), with a concomitant requirement to increase the complexity of studies to enable impact assessment at population, community and ecosystem levels, and to determine whether there are consequences for processes, such as parasite transmission, where MPs could vector parasites or increase infection susceptibility. This knowledge will determine the extent to which MP pollution can be considered a major anthropogenic stressor of freshwaters in this era of global environmental change.

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“…Larger PS particles at around 100 µm or above were shown not to have any significant effect in a number of studies [65][66][67]. MP/NP feeding can result in behavioral abnormalities in terms of feeding and movement of adults and larvae [35,[68][69][70][71][72][73][74], as well as reproduction in adults [75][76][77]. There is evidence of mother-offspring transfer of NPs [78], and that prenatal exposure of MPs affected early development of the neonates [77].…”

Section: Introductionmentioning

confidence: 99%

Toxicity of Microplastics and Nanoplastics in Mammalian Systems

Yong

Valiyaveettil

Tang

2020

IJERPH

555

280

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Fragmented or otherwise miniaturized plastic materials in the form of micro- or nanoplastics have been of nagging environmental concern. Perturbation of organismal physiology and behavior by micro- and nanoplastics have been widely documented for marine invertebrates. Some of these effects are also manifested by larger marine vertebrates such as fishes. More recently, possible effects of micro- and nanoplastics on mammalian gut microbiota as well as host cellular and metabolic toxicity have been reported in mouse models. Human exposure to micro- and nanoplastics occurs largely through ingestion, as these are found in food or derived from food packaging, but also in a less well-defined manner though inhalation. The pathophysiological consequences of acute and chronic micro- and nanoplastics exposure in the mammalian system, particularly humans, are yet unclear. In this review, we focus on the recent findings related to the potential toxicity and detrimental effects of micro- and nanoplastics as demonstrated in mouse models as well as human cell lines. The prevailing data suggest that micro- and nanoplastics accumulation in mammalian and human tissues would likely have negative, yet unclear long-term consequences. There is a need for cellular and systemic toxicity due to micro- and nanoplastics to be better illuminated, and the underlying mechanisms defined by further work.

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Acute toxic effects of polyethylene microplastic on adult zebrafish

Cited by 202 publications

References 96 publications

Embryotoxic and teratogenic effects of polyethylene microbeads found in facial wash products in Zebrafish (Danio rerio)using the Fish Embryo Acute Toxicity Test

Embryotoxic and teratogenic effects of polyethylene microbeads found in facial wash products in Zebrafish (Danio rerio)using the Fish Embryo Acute Toxicity Test

Microplastics in freshwater fishes: Occurrence, impacts and future perspectives

Toxicity of Microplastics and Nanoplastics in Mammalian Systems

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