Acute toxicity and genotoxicity studies on poly(ɛ-caprolactone)-poly(ethylene glycol)-poly(ɛ-caprolactone) nanomaterials

Huang, Yina; Gao, Hong; Gou, Maling; Ye, Huaxia; Liu, Yunke; Gao, Yuan; Peng, Feng; Zhang, Qian; Cen, Xiaobo; Zhao, Yinglan

doi:10.1016/j.mrgentox.2009.12.016

Cited by 34 publications

(10 citation statements)

References 24 publications

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“…Our conclusions demonstrated that ITO is negative in the Ames test, and these results are consistent with many studies that reported negative results in the Ames test (Balasubramanyam et al, 2010; Di Sotto et al, 2009; Huang et al, 2010; Kim et al, 2006; Kisin et al, 2007; Moreira et al, 2009; Nakagawa et al, 1997; Pan et al, 2010; Shinohara et al, 2009; Warheit et al, 2007; Yoshida et al, 2009) or weakly positive results (Pan et al, 2010; Sera et al, 1996).…”

Section: Discussionsupporting

confidence: 92%

Determination of mutagenicity and genotoxicity of indium tin oxide nanoparticles using the Ames test and micronucleus assay

et al. 2016

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In this study, the mutagenicity and genotoxicity of indium tin oxide (ITO) nanomaterial were assessed using two standard genotoxicity assays, the Salmonella reverse mutation assay (Ames test) and the in vitro micronucleus (MN) assay. Seven different concentrations (12.5, 25, 50, 75, 100, 125, and 150 µg/plate) of this nanomaterial were tested using the Ames test on the TA98 and TA100 strains in the presence and absence of the S9 mixture. At all the concentrations tested, this substance did not significantly increase the number of revertant colonies compared with the control with or without S9 mixture. The genotoxic effects of ITO were investigated in human peripheral lymphocytes treated with 125, 250, 500, and 750 µg/ml concentrations of this substance for 24- and 48-h treatment periods using an MN test. Nuclear division index (NDI) was also calculated in order to determine the cytotoxicity of ITO. It was determined that ITO increased MN frequency in the 750 µg/ml concentration in 24- and 48-h treatments. In addition, ITO dose dependently decreased the NDI significantly for two treatment periods.

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Section: Discussionsupporting

confidence: 92%

Determination of mutagenicity and genotoxicity of indium tin oxide nanoparticles using the Ames test and micronucleus assay

et al. 2016

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“…Nanotoxicology studies mechanisms of cytotoxicity of nanomaterials while nanogenotoxicology is focused on analyzing the potential of engineered nanomaterials on damaging DNA. While cytotoxicity of nanocarriers is relatively widely analyzed for different nanomaterials, genotoxicity of nanocarriers were investigated only in limited number of recent publications [3][4][5][7][8][9][10][11][12][13][14][15]. Meanwhile, genotoxicity of drug carriers should be avoided especially for prolonged chronic treatment.…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity of Different Nanocarriers: Possible Modifications for the Delivery of Nucleic Acids

Shah¹,

Taratula²,

Garbuzenko³

et al. 2013

Current Drug Discovery Technologies

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The prevention of cyto- and genotoxicity of nanocarriers is an important task in nanomedicine. In the present investigation, we, at the first time using similar experimental conditions, compared genotoxicity of nanocarriers with different composition, architecture, size, molecular weight and charge. Poly(ethylene glycol) polymers, neutral and cationic liposomes, micelles, poly(amindo amine) and poly(propyleneimine) dendrimers, quantum dots, mesoporous silica, and supermagnetic iron oxide (SPIO) nanoparticles were studied. All nanoparticles were used in non-cytotoxic concentrations. However, even in these concentrations, positively charged cationic liposomes, dendrimers, and SPIO nanoparticles induced genotoxicity leading to the significant formation of micronuclei in cells. Negatively charged and neutral nanocarriers were not genotoxic. A strong positive correlation was found between the number of formed micronuclei and the positive charge of nanocarriers. We proposed modifications of both types of dendrimers and SPIO nanoparticles that substantially decreased their genotoxicity and allowed for an efficient intracellular delivery of nucleic acids.

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“…Because of the simplicity and efficacy, the micronucleus test has become a popular and useful in vivo procedure for the investigation of polymer‐induced chromosome damage . In this micronucleus test, no abnormal signs in general appearance and body weight were observed in mice over the first and final administrations in all groups.…”

Section: Resultsmentioning

confidence: 99%

Toxicity andin vivobiological effect of the nanoparticular self-supported hydrogel of a thermosensitive copolymer for non-invasive drug delivery

Wang

Deng

Huang

et al. 2013

J. Biomed. Mater. Res.

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Injectable thermosensitive hydrogels provide local non-invasive platforms for sustained drug release,tissue engineering and cellular immunity. As a long-term implant, the toxicity and in vivo biological effect should be concerned.Previously we developed a novel type of injectable nanoparticular self-supported hydrogel (PECT NPs(Gel))of PEG and pendent cycle ethers modified poly(ε-caprolactone) triblock copolymer (PECT), which could sustainedly release PECT or drug-loaded PECT nanoparticles with the hydrogel disassembly and provided efficient antitumor activity and significant decrease of side effects. Herein, the aim of this work was to reveal the toxicity and in vivo biological effect of PECT nanoparticles and PECT NPs(Gel). In vitro cytotoxicity indicated no cell cytotoxicity was observed when the concentration of PECT nanoparticle was up to 500 µg/mL, and also nomutagenic effect and no genotoxicity were observed.In vivo intravenous injection of PECT nanoparticles demonstrated that the LD50 was approximate high to 2.564 g/kg, and compared with the control mice, the mice treated with daily administration of PECT nanoparticles showed no difference in the physical or behavioral alterations, body weight changes, biochemical and hematological parameters as well as organ coefficients. The in vivo chronic effect of PECT NPs(Gel)confirmed no toxic lesions to animals in a whole period of three months even the dosage was high to 20 g/kg. These findings indicated PECT nanoparticles and PECT NPs(Gel) were of well biocompatibility and did not provoke any side effect to body, which represented a new class of injectable and non-invasive systemic or site-specific delivery carrier.

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Acute toxicity and genotoxicity studies on poly(ɛ-caprolactone)-poly(ethylene glycol)-poly(ɛ-caprolactone) nanomaterials

Cited by 34 publications

References 24 publications

Determination of mutagenicity and genotoxicity of indium tin oxide nanoparticles using the Ames test and micronucleus assay

Determination of mutagenicity and genotoxicity of indium tin oxide nanoparticles using the Ames test and micronucleus assay

Genotoxicity of Different Nanocarriers: Possible Modifications for the Delivery of Nucleic Acids

Toxicity andin vivobiological effect of the nanoparticular self-supported hydrogel of a thermosensitive copolymer for non-invasive drug delivery

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