Acute toxicity and morphology alterations of glyphosate-based herbicides to Daphnia magna and Cyclops vicinus

Gustinasari, Kiki; Sługocki, Łukasz; Czerniawski, Robert; Pandebesie, Ellina Sitepu; Hermana, Joni

doi:10.1007/s43188-020-00054-1

Cited by 16 publications

(3 citation statements)

References 23 publications

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“…There have long been concerns over its implications in the environment and for public health. Glyphosate has been shown to cause morphological alterations in zooplanctonic organisms and crustaceans [ 42 ], and to impact carbon and fat metabolism and the microbiome [ 43 ] as well as the heart rate of daphnids [ 44 ]. Our results support these findings, as an increase in lipase and peptidase was observed along with a decrease in alkaline phosphatase and GST.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Single and Combined Stressors on Daphnids—Enzyme Markers of Physiology and Metabolomics Validate the Impact of Pollution

Michalaki

McGivern²,

Poschet³

et al. 2022

Toxics

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The continuous global increase in population and consumption of resources due to human activities has had a significant impact on the environment. Therefore, assessment of environmental exposure to toxic chemicals as well as their impact on biological systems is of significant importance. Freshwater systems are currently under threat and monitored; however, current methods for pollution assessment can neither provide mechanistic insight nor predict adverse effects from complex pollution. Using daphnids as a bioindicator, we assessed the impact in acute exposures of eight individual chemicals and specifically two metals, four pharmaceuticals, a pesticide and a stimulant, and their composite mixture combining phenotypic, biochemical and metabolic markers of physiology. Toxicity levels were in the same order of magnitude and significantly enhanced in the composite mixture. Results from individual chemicals showed distinct biochemical responses for key enzyme activities such as phosphatases, lipase, peptidase, β-galactosidase and glutathione-S-transferase. Following this, a more realistic mixture scenario was assessed with the aforementioned enzyme markers and a metabolomic approach. A clear dose-dependent effect for the composite mixture was validated with enzyme markers of physiology, and the metabolomic analysis verified the effects observed, thus providing a sensitive metrics in metabolite perturbations. Our study highlights that sensitive enzyme markers can be used in advance on the design of metabolic and holistic assays to guide the selection of chemicals and the trajectory of the study, while providing mechanistic insight. In the future this could prove to become a useful tool for understanding and predicting freshwater pollution.

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Section: Discussionmentioning

confidence: 99%

The Effects of Single and Combined Stressors on Daphnids—Enzyme Markers of Physiology and Metabolomics Validate the Impact of Pollution

Michalaki

McGivern²,

Poschet³

et al. 2022

Toxics

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“…Testing with Daphnia sp. is one of the most used approaches in the toxicity assessment of different chemical compounds, including pharmaceuticals [ 47 ]. Moreover, using organisms from Daphnia species cultured through parthenogenesis ensures significantly reduced variability [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Profile, Antioxidant and Cytotoxic Potential of Capsicum annuum (L.) Dry Hydro-Ethanolic Extract

Ivan,

Popovici,

Chițescu

et al. 2024

Pharmaceutics

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Capsicum annuum (L.) is one of the essential spices most frequently used in our daily routine and has remarkable ethnobotanical and pharmacological properties. Its fruits are rich in vitamins, minerals, carotenoids, and numerous other phenolic metabolites with a well-known antioxidant activity. Regular consumption of chili fruits may have a positive influence on human health. Therefore, we investigated a commercially available chili fruit powder in the present study, extracting it with 50% ethanol. The dried hydro-ethanolic extract (CAE) was thoroughly analyzed using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC–HRMS/MS), and 79 bioactive phenolic constituents were identified. Then, we quantified the main phenolic compounds and found a polyphenol content of 4.725 ± 1.361 mg Eq tannic acid/100 g extract and a flavonoid amount of 1.154 ± 0.044 mg Eq rutin/100 g extract. Phenolic secondary metabolites are known for their dual redox behavior as antioxidants/pro-oxidants, underlying their numerous benefits in health and disease. Thus, the antioxidant potential of CAE was evaluated using three methods; our results could explain the protective effects of chili fruits: IC50DPPH = 1.669 mg/mL, IC50ABTS = 0.200 mg/mL, and EC50FRAP = 0.561 mg/mL. The pro-oxidant potential of phenolic compounds could be a basis for CAE cytotoxicity, investigated in vitro on tumor cell lines and in vivo on Daphnia sp. Results demonstrated the dose- and time-dependent CAE’s cytotoxic activity; the highest antiproliferative activity was recorded on colon (LoVo) and breast (MDA-MB-231) cancer cell lines after 48 h of exposure (IC50 values < 200 µg/mL). In vivo testing on Daphnia sp. reported a potent CAE cytotoxicity after 48 h and embryonic developmental delays. Extensive data analyses support our results, showing a significant correlation between the CAE’s concentration, phenolic compound content, antioxidant activity, exposure time, and the viability rate of different tested cell lines.

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“…Although the differences were not signi cant, these results suggest that cladocerans accelerate their growth in the presence of some stressors, such as some herbicides, and that higher doses of glyphosate can cause the necessity for females to be bigger to release their rst offspring. In contrast, Gustinasari et al (2020) studied the effects of glyphosate on morphological aspects of D. magna but did not observe changes in body length until the concentration exceeded 320 mg L − 1 . The results provided here highlight the importance of trade-off processes in regulating the distribution of stored energy towards growth and reproduction in daphnids (Knops et al 2001).…”

Section: Recovery Testmentioning

confidence: 96%

Sensitivity of Daphnia spinulata Birabén, 1917 to glyphosate at different salinity levels

Lares,

Vignatti,

Echaniz

et al. 2024

Environ Sci Pollut Res

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Daphnia spinulata Birabén, 1917 is an endemic cladoceran species, frequent in the zooplankton communities of the shallow lakes of the Pampean region of Argentina. These lakes have varying salinity levels and, being located in agricultural areas, are frequently subject to pesticide pollution. This study aimed to determine the effects of the herbicide glyphosate (Panzer Gold®) in combination with different salinity levels on the biological parameters of D. spinulata and its recovery ability after a short exposure. Three types of assays were performed: an acute toxicity test, a chronic assessment to determine survival, growth and reproduction, and recovery assays under optimal salinity conditions (1 g L − 1 ). The LC 50 -48h of glyphosate was 7.5 mg L − 1 (3. 15-11.72). Longevity and the number of offspring and clutches were signi cantly reduced by glyphosate and high salinity exposure, whether in isolation or combined. The timing of the rst offspring did not recover after glyphosate exposure. Our results reveal that D. spinulata is sensitive to the herbicide, which causes stronger negative effects in conditions of higher salinity. Further research is needed to shed further light on the sensitivity of this cladoceran to glyphosate and its variability under other interactive stress factors.

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Acute toxicity and morphology alterations of glyphosate-based herbicides to Daphnia magna and Cyclops vicinus

Cited by 16 publications

References 23 publications

The Effects of Single and Combined Stressors on Daphnids—Enzyme Markers of Physiology and Metabolomics Validate the Impact of Pollution

The Effects of Single and Combined Stressors on Daphnids—Enzyme Markers of Physiology and Metabolomics Validate the Impact of Pollution

Phytochemical Profile, Antioxidant and Cytotoxic Potential of Capsicum annuum (L.) Dry Hydro-Ethanolic Extract

Sensitivity of Daphnia spinulata Birabén, 1917 to glyphosate at different salinity levels

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