Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats

Guševac Stojanović, Ivana; Drakulić, Dunja; Todorović, Ana; Martinović, Jelena; Filipović, Nenad; Stojanović, Zoran

doi:10.3390/toxics12030167

Cited by 4 publications

(1 citation statement)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have shown that due to their accumulation in cells and tissues, NPs/MPs can induce cytotoxicity [17], oxidative stress [18], genotoxicity [19], inflammation [20], and neurotoxicity [21], among others [22,23]. Hence, the aim of the present work was to describe the factors that increase the cytotoxicity of plastic NPs/MPs in vitro, with regard to cell viability, cell death, reactive oxygen species (ROS) induction, and genotoxicity.…”

Section: Introductionmentioning

confidence: 97%

Important Factors Affecting Induction of Cell Death, Oxidative Stress and DNA Damage by Nano- and Microplastic Particles In Vitro

Płuciennik,

Sicińska,

Misztal

et al. 2024

Cells

View full text Add to dashboard Cite

We have described the influence of selected factors that increase the toxicity of nanoplastics (NPs) and microplastics (MPs) with regard to cell viability, various types of cell death, reactive oxygen species (ROS) induction, and genotoxicity. These factors include plastic particle size (NPs/MPs), zeta potential, exposure time, concentration, functionalization, and the influence of environmental factors and cell type. Studies have unequivocally shown that smaller plastic particles are more cytotoxic, penetrate cells more easily, increase ROS formation, and induce oxidative damage to proteins, lipids, and DNA. The toxic effects also increase with concentration and incubation time. NPs with positive zeta potential are also more toxic than those with a negative zeta potential because the cells are negatively charged, inducing stronger interactions. The deleterious effects of NPs and MPs are increased by functionalization with anionic or carboxyl groups, due to greater interaction with cell membrane components. Cationic NPs/MPs are particularly toxic due to their greater cellular uptake and/or their effects on cells and lysosomal membranes. The effects of polystyrene (PS) vary from one cell type to another, and normal cells are more sensitive to NPs than cancerous ones. The toxicity of NPs/MPs can be enhanced by environmental factors, including UV radiation, as they cause the particles to shrink and change their shape, which is a particularly important consideration when working with environmentally-changed NPs/MPs. In summary, the cytotoxicity, oxidative properties, and genotoxicity of plastic particles depends on their concentration, duration of action, and cell type. Also, NPs/MPs with a smaller diameter and positive zeta potential, and those exposed to UV and functionalized with amino groups, demonstrate higher toxicity than larger, non-functionalized and environmentally-unchanged particles with a negative zeta potential.

show abstract

Section: Introductionmentioning

confidence: 97%

Important Factors Affecting Induction of Cell Death, Oxidative Stress and DNA Damage by Nano- and Microplastic Particles In Vitro

Płuciennik,

Sicińska,

Misztal

et al. 2024

Cells

View full text Add to dashboard Cite

show abstract

Impact of artificial sunlight aging on the respiratory effects of polyethylene terephthalate microplastics through degradation-mediated terephthalic acid release in male mice

Ishihara,

Kajino,

Iwamoto

et al. 2024

Toxicological Sciences

View full text Add to dashboard Cite

Microplastics are ubiquitous in the atmosphere, leading to human exposure through inhalation. Airborne microplastics undergo degradation due to sunlight irradiation, yet the respiratory risks associated with degraded microplastics remain poorly understood. In this study, we investigated the respiratory effects of polyethylene terephthalate (PET) degraded by artificial sunlight and created a transport and degradation model of PET for risk assessment. PET fibers were cut and subjected to artificial sunlight irradiation. Mice exposed to aged PET showed increased airway resistance induced by methacholine inhalation, along with lung inflammation and neutrophil infiltration. Terephthalic acid (TPA) was continuously released from PET aged by artificial sunlight. Exposure to TPA also caused lung inflammation and enhanced airway resistance induced by methacholine (MCh) in mice. These findings indicate that aged PET can cause respiratory impairment via TPA release. A simple transport and degradation model was developed to quantitatively relate the abundance of aged PET produced in this study (ie, 4,000 × 96 W m−2 h) and aged fractions of PET that can be generated in the atmosphere. Our results suggested 10-60% of PET was degraded as that produced in this study over sunny regions in summer, whereas only lower than 1% in high-latitude cities in Europe in winter. This study demonstrates the importance of considering the abundance of aged PET and further development of a transport and degradation model of PET to assess the risk of degraded PET in the atmosphere.

show abstract

In Vitro and In Vivo Genotoxicity of Polystyrene Microplastics: Evaluation of a Possible Synergistic Action with Bisphenol A

Santovito,

Lambertini,

Nota

2024

JoX

View full text Add to dashboard Cite

The ubiquitous presence of plastics represents a global threat for all ecosystems and human health. In this study, we evaluated, in vitro and in vivo, the genotoxic potential of different concentrations of polystyrene microplastics (PS-MPs) and their possible synergistic interactions with bisphenol-A (BPA). For the in vitro and the in vivo assays, we used human lymphocytes and hemocytes from Lymnaea stagnalis, respectively. The genomic damage was evaluated by the micronucleus assay, and differences in eggs laid and growth of L. stagnalis were also evaluated. In human lymphocytes, PS-MPs alone at the concentration of 200 μg/mL and in association with BPA 0.100 µg/mL significantly increased the frequencies of micronuclei and nuclear buds, indicating a possible in vitro genotoxic additive action of these two compounds. Vice versa, PS-MPs did not result in genotoxicity in hemocytes. Our results indicated that PS-MPs have genotoxic properties only in vitro and at a concentration of 200 µg/mL; moreover, this compound could intensify the genomic damage when tested with BPA, indicating possible cumulative effects. Finally, PS significantly reduced the growth and the number of laid eggs in L. stagnalis.

show abstract

Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats

Cited by 4 publications

References 53 publications

Important Factors Affecting Induction of Cell Death, Oxidative Stress and DNA Damage by Nano- and Microplastic Particles In Vitro

Important Factors Affecting Induction of Cell Death, Oxidative Stress and DNA Damage by Nano- and Microplastic Particles In Vitro

Impact of artificial sunlight aging on the respiratory effects of polyethylene terephthalate microplastics through degradation-mediated terephthalic acid release in male mice

In Vitro and In Vivo Genotoxicity of Polystyrene Microplastics: Evaluation of a Possible Synergistic Action with Bisphenol A

Contact Info

Product

Resources

About