Acute toxicity evaluation of drought-tolerant transgenic rice Agb0103 to Daphnia magna

Abstract

A drought-tolerant transgenic rice (Agb0103) was developed using a pepper methionine sulfoxide reductase (CaMsrB2) under the control of rice Rab21 promoter with a selection marker, the phosphinothricin acetyltransferase (PAT) gene. Commercialization of genetically modified (GM) crops will require the evaluation of risks associated with the release of GM crops. With the potential problems associated to GM crops safety testing, the investigation of their effects on non-target organisms is necessary for environmental risk research. This study was carried out to assess acute toxicity of a GM crop using the water flea (Daphnia magna) for non-target organism risk evaluation. The effect of acute toxicity on Daphnia magna of Agb0103 rice and a non-GM rice, Ilmibyeo, were investigated at different concentrations (0, 625, 1,250, 2,500, 5,000, and 10,000 mg/L). The Agb0103 rice used for the test was confirmed to express the CaMsrB2/PAT gene by the PCR and ELISA. Daphnia magna feeding tests showed no significant differences in cumulative immobility or abnormal response with either Agb0103 rice or non-GM rice. The 48hr-EC 50 values showed no difference between Agb0103 rice (2243 mg/L) and non-GM rice (2694 mg/L). These results suggest that there is no significant difference in toxicity to Daphnia magna between Agb0103 rice and its non-GM counterpart.

Application of simple and massive purification system of dsRNA in vivo for acute toxicity to Daphnia magna

“…), and drought tolerant transgenic rice (Agb0103, Oh et al . ) were developed and risk assessments were performed on for environmental release in Korea. These studies announced that transgenic rice did not show any difference compared to non‐GM rice in an acute toxicity test (48 hr‐EC 50 ).…”

“…Biosafety assessment of genetically modified rice to D. magna has been performed many times to investigate the effects on non-target organisms. Insect resistant transgenic rice (mCry1Ac1, Oh et al 2011), disease resistant rice (OsCK1, Oh et al 2014), and drought tolerant transgenic rice (Agb0103, Oh et al 2016) were developed and risk assessments were performed on for environmental release in Korea. These studies announced that transgenic rice did not show any difference compared to non-GM rice in an acute toxicity test (48 hr-EC 50 ).…”