Acute toxicity of aqueous extract of Ambrosia arborescens Mill. on biochemical and histopathological parameters in rats

Silva-Correa, Carmen R.; Torre, Víctor E. Villarreal-La; González-Siccha, Anabel D.; Cruzado-Razco, José L.; González-Blas, María V.; Sagástegui-Guarniz, William Antonio; Calderón-Peña, Abhel A.; Aspajo-Villalaz, Cinthya L.; Hilario-Vargas, Julio

doi:10.1007/s43188-021-00106-0

Cited by 6 publications

(6 citation statements)

References 37 publications

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“…In relation to the phytochemistry and biological activities of A. arborescens, Hilario-Vargas et al reported the acute toxicity of an aqueous extract of the leaves of A. arborescens Mill at a single dose of 300 and 2000 mg/kg in rats. The study showed that the aqueous extract had an LD 50 greater than 2000 mg/kg, causing mild congestion in the liver and kidneys based on creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and histopathological examinations [17]. Another work indicated that the sesquiterpene lactones coronopilin and damsin from Bolivian A. arborescens inhibited the expression of the pro-inflammatory cytokines IL-6 and MCP-1 via NF-κB inhibition, suggesting that they could be useful as anti-inflammatory agents for human skin [18].…”

Section: Antioxidant Activitymentioning

confidence: 99%

Microwave-Assisted Extraction of Secondary Metabolites Using Ethyl Lactate Green Solvent from Ambrosia arborescens: LC/ESI-MS/MS and Antioxidant Activity

Guillen,

Terrones,

de Terrones

et al. 2024

Plants

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Alternative solvents are being tested as green solvents to replace the traditional organic solvents used in both academy and industry. Some of these are already available, such as ethyl lactate, cyrene, limonene, glycerol, and others. This alternative explores eco-friendly processes for extracting secondary metabolites from nature, thus increasing the number of unconventional extraction methods with lower environmental impact over conventional methods. In this context, the Peruvian Ambrosia arborescens was our model while exploring a microwave-assisted extraction (MAE) approach over maceration. The objective of this study was to perform a phytochemical study including UHPLC-ESI-MS/MS and the antioxidant activity of Ambrosia arborescens, using sustainable strategies by mixing both microwaves and ethyl lactate as a green solvent. The results showed that ethyl lactate/MAE (15.07%) achieved a higher extraction yield than methanol/maceration (12.6%). In the case of the isolation of psilostachyin, it was similar to ethyl lactate (0.44%) when compared to methanol (0.40%). Regarding UHPLC-ESI-MS/MS studies, the results were similar. Twenty-eight compounds were identified in the ethyl lactate/MAE and methanol/maceration extracts, except for the tentative identification of two additional amino acids (peaks 4 and 6) in the MeOH extract. In relation to the antioxidant assay, the activity of the ethyl lactate extract was a little higher than the methanol extract in terms of ORAC (715.38 ± 3.2) and DPPH (263.04 ± 2.8). This study on A. arborescens demonstrated that the unconventional techniques, such as MAE related to ethyl lactate, could replace maceration/MeOH for the extraction and isolation of metabolites from diverse sources. This finding showed the potential of unconventional methods with green solvents to provide eco-friendly methods based on green chemistry.

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Section: Antioxidant Activitymentioning

confidence: 99%

Microwave-Assisted Extraction of Secondary Metabolites Using Ethyl Lactate Green Solvent from Ambrosia arborescens: LC/ESI-MS/MS and Antioxidant Activity

Guillen,

Terrones,

de Terrones

et al. 2024

Plants

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“…The body weight of the rats was measured three times, once before administering the extract and on days 7 and 14 of observation. 19 The animal study protocol was approved by the Ethics Committee of the Faculty of Medicine the Universidad Nacional de Trujillo (Approval Certificate No. : 372 -2022/ UNT-FM-C.E.…”

Section: Acute Toxicity Testmentioning

confidence: 99%

“…The biochemical parameters that were measured are urea, creatinine, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), cholesterol, and triglycerides. 19…”

Section: Blood Biochemistry Evaluationmentioning

confidence: 99%

Evaluation of the Acute Toxicity of the Ethanolic Extract of the Rhizome of Zingiber officinale Roscoe in Rats

Villarreal-La Torre,

Chávez-Flores,

Silva-Correa

et al. 2024

Pharmacogn J.

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Ginger (Zingiber officinale) is an herbaceous, perennial, rhizomatous plant that belongs to the Zingiberaceae family. 1 This plant is endemic to India and cultivated in South and South-East Asia, Africa, Latin America and Australia. 2 The rhizome of ginger has been processed into powder, syrup, volatile oil, and oleoresin. Clinical trials indicate that ginger can be ingested to alleviate nausea and vomiting induced by surgery, pain, diabetes, obesity, inflammation, male infertility, flatulent intestinal colic, indigestion, insomnia, to treat memory loss, rheumatism, stomach pain, and urinary tract infections. [3][4] Ginger has pharmacological activities such as antidiabetic 5 , antioxidant 6 , anticancer 7 , antineuroinflammatory 8 , antibacterial 9-11 , antiobesity 12 , immunomodulatory and antiallergic. 13 There are many compounds isolated from ginger. These can be classified into seven categories, which cover volatile oils such as α-zingiberene, which is the main component of the volatile oil of Z. officinale 14 , terpenoid analogues consisting mainly of monoterpene and sesquiterpene 15 , flavonoids 14 , gingerol analogues such as gingerol, shogaol, zingerone, paradol, gingerdione and gingerdiol 16 , diarylheptanoids that are a class of compounds with 1,7-disubstituted phenyl groups and a heptane structure, thus we have curcumin 14 , organic acids that can be differentiated into fatty acids, aromatic acids, oxalic and tartaric acids 17 , steroids, such as daucosterol and 24-propyl cholesterol. Gingerols are the main functional components and the characteristic aromatic ingredients of the Zingiber genus. 16 Plant species are currently important for their various therapeutic uses and play a significant role in traditional medicine. So, an evaluation of their safety profile must be carried out through toxicological studies. This study was carried out to evaluate the acute toxicity of the ethanolic extract of the rhizomes of Zingiber officinale on the biochemical and histopathological parameters of Albino Holtzman rats. MATERIALS AND METHODS Collection of the plant speciesThe rhizomes of Zingiber officinale Roscoe were collected in the town of Alto Toterani annex, Perenne district, Chanchamayo Province, Junín Department, Peru. The selected plant species were taken to the Natural History Museum of the Universidad Nacional Mayor de San Marcos for taxonomic identification (Proof N°293-UNMSM-2018).

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“…These findings were agreed with the work of [24] who investigated the acute toxicity test of Atalantia guillauminii and found safe for consumption. The ethanolic extracts of the aerial parts of Phragmites karka was evaluated to be safe as recorded by [25,26] reported that Ambrosia arborescens extract was examined for acute toxicity showed no mortality.…”

Section: Acute Toxicity Testmentioning

confidence: 99%

Pharmacological Activities of Rhododendron afghanicum; an Endemic Species of Khyber Pakhtunkhwa, Pakistan

Alam,

Ahmad,

Ahmad

et al. 2023

Chemistry & Biodiversity

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Majority of different kinds of metabolites having therapeutic characteristics are thought to be stored in medicinal plants. So, the present study was aimed to explore the crude extract of leaves and stem of R. afghanicum for phytochemical screening and various pharmacological activities. Toxicological studies at 100 mg/kg showed 60 % mortality where its safe dose level was 90 mg/kg. Phytochemical screening revealed the presence of alkaloids, glycosides, flavonoids and tannins in both extracts. Bacterial strains were susceptible to (RLEt) and (RLM) crude extracts except Staphylococcus aureus. RSM showed maximum anti‐inflammatory activity (20.16 %) followed by RSEt (20.14 %) where lowest activity was displayed by RLEt (18.46 %). Phytotoxic activity showed a substantial dose‐dependent phyto‐inhibition of Lemna minor. An outstanding cytotoxic potential was displayed with LD50 values of 9.46 and 13.03 μg/ml in both stem extracts. RLEt demonstrated a dose‐dependent pain relief at 30, 60 and 90 mg/kg which was 31 %, 40 % and 52 % respectively. A considerable spasmolytic action was observed by the shrinkage of jejunum muscle in albino mice. RLEt at 1000 ppm showed (17 mm) and RLM at 1000 ppm showed (16 mm) zone of inhibition against Aspergillus niger. These findings support and corroborate the traditional applications of R. afghanicum for treating digestive, analgesic and inflammatory ailments.

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Acute toxicity of aqueous extract of Ambrosia arborescens Mill. on biochemical and histopathological parameters in rats

Cited by 6 publications

References 37 publications

Microwave-Assisted Extraction of Secondary Metabolites Using Ethyl Lactate Green Solvent from Ambrosia arborescens: LC/ESI-MS/MS and Antioxidant Activity

Microwave-Assisted Extraction of Secondary Metabolites Using Ethyl Lactate Green Solvent from Ambrosia arborescens: LC/ESI-MS/MS and Antioxidant Activity

Evaluation of the Acute Toxicity of the Ethanolic Extract of the Rhizome of Zingiber officinale Roscoe in Rats

Pharmacological Activities of Rhododendron afghanicum; an Endemic Species of Khyber Pakhtunkhwa, Pakistan

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