Acute Toxicity of Deltamethrin on the Fatty Acid Composition of Phospholipid Classes in Liver and Gill Tissues of Nile tilapia

Cengiz, Elif İpek; Bayar, Ahmet Serhat; Kizmaz, Veysi; Başhan, Mehmet; Satar, Ali

doi:10.1007/s41742-017-0034-2

Cited by 17 publications

(7 citation statements)

References 20 publications

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“…Design of “green pesticides” is based on the structure of naturally occurring substances synthesized by plants to protect them against pests. However, these novel generations of pesticides such as pyrethroids and neonicotinoids are still able to exhibit adverse effects on animals and humans (Cengiz et al, ; Cimino et al, ).…”

Section: Introductionmentioning

confidence: 99%

Chromosome Missegregation and Aneuploidy Induction in Human Peripheral Blood Lymphocytes In vitro by Low Concentrations of Chlorpyrifos, Imidacloprid and α‐Cypermethrin

Mužinić

Ramić

Želježić

2018

Environ and Mol Mutagen

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Chlorpyrifos, imidacloprid, and α‐cypermethrin are some of the most widely used insecticides in contemporary agriculture. However, their low‐dose, nontarget genotoxic effects have not been extensively assayed. As one of the most relevant cancer biomarkers, we aimed to assess the aneuploidy due to chromosome missegregation during mitosis. To aim it we treated human lymphocytes in vitro with three concentrations of insecticides equivalents relevant for real scenario exposure assessed by regulatory agencies. We focused on chlorpyrifos as conventional and imidacloprid and α‐cypermethrin as sustainable use insecticides. Cytokinesis‐blocked micronucleus assay was performed coupled with fluorescence in situ hybridization (FISH) with directly labeled pancentromeric probes for chromosomes 9, 18, X and Y. None of the insecticides induced significant secondary DNA damage in terms of micronuclei (MN), nuclear buds (NB), or nucleoplasmic bridges (NPB). However, significant disbalances in chromosomes 9, 18, X and Y, and in insecticide‐treated cells has been observed. According to recent studies, these disbalances in chromosome numbers may be atributted to defect sister chromatid cohesion which contribute to the increase of chromosome missegregation but not to micronuclei incidence. We conclude that tested insecticidal active substances exert chromosome missegregation effects at low concentrations, possibly by mechanism of sister chromatid cohesion. These findings may contribute to future risk assesments and understanding of insecticide mode of action on human genome. Environ. Mol. Mutagen. 60:72–84, 2019. © 2018 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 99%

Chromosome Missegregation and Aneuploidy Induction in Human Peripheral Blood Lymphocytes In vitro by Low Concentrations of Chlorpyrifos, Imidacloprid and α‐Cypermethrin

Mužinić

Ramić

Želježić

2018

Environ and Mol Mutagen

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“…Cengiz et al (2016) tarafından yapılan bir çalışmada deltametrinin PC üzerindeki etkisi total SFAs ve total PUFAs üzerinde bulunmuştur. Yapılan başka bir çalışmada ise total SFAs, total MUFAs ve total PUFAs da anlamlı bir değişme gözlenmemiştir (Cengiz et al, 2017).…”

Section: Hücresel Membran Yapısında Membran Lipitlerininunclassified

“…Cengiz et al (2016) tarafından yapılan çalışmalarda da aynı sonuçlar gözlenmiştir. Başka bir çalışmada ise deltametrinin PI üzerinde total SFAs, total MUFAs ve total PUFAs da anlamlı değişiklikler tespit edilmemiştir (Cengiz et al, 2017).…”

Section: Hücresel Membran Yapısında Membran Lipitlerininunclassified

Lambda-cyhalothrin'nin Oreochromis niloticus’un Solungaç Fosfolipid (PL) Alt Sınıfı Üzerine Etkisi

YOLCU,

SATAR

2023

Comm. J. Biol.

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Fatty acids have a crucial role in providing energy and performing essential functions in living organisms. Moreover, these substances exhibit the most significant alterations in their structure based on ecotoxicological parameters when viewed from a biochemical perspective. These bioactive chemicals are present in the cellular architecture. The study of these fatty acids, crucial for maintaining the integrity and permeability of cell membranes, holds great significance for all living organisms. Consequently, doing fatty acid analysis specifically at the phospholipid level holds significant importance. The impact of lambda cyhalothrin on the fatty acid content of several phospholipid subclasses (phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS) in the gill tissue of O. niloticus (Perciformes: Cichlidae) was assessed using gas chromatography. The alterations in the fatty acid composition was analyzed 21 days after exposure. Following the complete extraction of lipids from gill tissue, the tissue was subsequently separated into different subclasses of phospholipids using thin layer chromatography. The samples were subjected to methylation and then evaluated using Gas Chromatography to determine the percentage of the fatty acid. After doing the analysis, a grand total of 16 fatty acids were identified. The research revealed that the primary fatty acids were 16:0 and 18:0 of saturated fatty acids, monounsaturated 18:1n-9, and polyunsaturated 18:2n-6, 20:4n-6, and 22:6n-6. Upon analyzing the distribution of fatty acids, it was observed that PC, PE, and PI included 16:0, PE contained 18:1, PE and PS contained C18:2n-6 and 20:4n-6, and significant alterations in C22:6n-3 were detected in PE. Our investigation revealed that the n-3/n-6 ratio of fish in the PE subclass was the lowest when compared to PC, PI, and PS.

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“…The prepared diets were fed to Nile tilapia with or without DMT ambient exposure (15 µg/L) (98.5%, Kafr El-Zayat Company for Chemicals and Pesticides, El-Gharbeya, Egypt) for 30 days. The doses of the synbiotic mixture and DMT were proposed by following the methods of Dawood et al [30] and Cengiz et al [42], respectively. Fish were collected from a local farm and transferred to Sakha Aquaculture Research Unit, Kafrelsheikh, Egypt.…”

Section: Fish Diets and Experimental Designmentioning

confidence: 99%

Dietary Synbiotics Can Help Relieve the Impacts of Deltamethrin Toxicity of Nile Tilapia Reared at Low Temperatures

Gewaily

Abdo

Moustafa

et al. 2021

Animals

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The optimal water temperature for the normal growth of Nile tilapia is between 26 and 28 °C, and the toxicity of pesticides is strongly related to water temperature. An alternate approach to augmenting the resistance of fish to ambient water toxicity and low water temperature via synbiotic feeding was proposed. In this study, fish were allocated into four groups with 10 fish in each replicate, where they were fed a basal diet or synbiotics (550 mg/kg) and kept at a suboptimal water temperature (21 ± 2 °C). The prepared diets were fed to Nile tilapia for 30 days with or without deltamethrin (DMT) ambient exposure (15 μg/L). The groups were named control (basal diet without DMT toxicity), DMT (basal diet with DMT toxicity), synbiotic (synbiotics without DMT toxicity), and DMT + synbiotic (synbiotics with DMT toxicity). The results displayed upregulated transcription of catalase, glutathione peroxidase, and interferon (IFN-γ) genes caused by DMT exposure and synbiotic feeding when compared with the controls. Moreover, HSP70 and CASP3 genes displayed increased transcription caused by DMT exposure without synbiotic feeding. However, fish fed with synbiotics showed downregulated HSP70 and CASP3 gene expressions. The transcription of IL-1β and IL-8 genes were also decreased by DMT exposure, while fish fed synbiotics showed upregulated levels. DMT exposure resulted in irregular histopathological features in gills, intestine, spleen, and liver tissues, while fish fed synbiotics showed regular, normal, and protected histopathological images. Our results indicated that dietary synbiotics ameliorated histopathological damages in DMT-exposed tilapia through alleviation of oxidative stress and inflammation as well as enhancing the immunity.

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Acute Toxicity of Deltamethrin on the Fatty Acid Composition of Phospholipid Classes in Liver and Gill Tissues of Nile tilapia

Cited by 17 publications

References 20 publications

Chromosome Missegregation and Aneuploidy Induction in Human Peripheral Blood Lymphocytes In vitro by Low Concentrations of Chlorpyrifos, Imidacloprid and α‐Cypermethrin

Chromosome Missegregation and Aneuploidy Induction in Human Peripheral Blood Lymphocytes In vitro by Low Concentrations of Chlorpyrifos, Imidacloprid and α‐Cypermethrin

Lambda-cyhalothrin'nin Oreochromis niloticus’un Solungaç Fosfolipid (PL) Alt Sınıfı Üzerine Etkisi

Dietary Synbiotics Can Help Relieve the Impacts of Deltamethrin Toxicity of Nile Tilapia Reared at Low Temperatures

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