Acute Toxicity Study of Durian Mesocarpium Ethanol Extracts (Durio Zibethinus Linn) in Healthy Mice

Abstract

Durian (Durio zibethinus Linn.) has been shown to have toxicity effect on acute toxicity in vivo. Objective: This study aimed to determine the acute toxicity of the Durian Mesocarpium Ethanol Extracts (EEMD), various doses of Durian Mesocarpium Ethanol Extracts (EEMD) by examining in vivo to the healthy mice experimentally. The toxic effects appeared in a short time after 24 hours by oral administrations of the dosages. This study used completely randomized design. The animals used in this study were mice (Swiss Webster), with criteria are healthy, male, and the range of the ages were 2 -3 months with the body weight 20 -30 g. The various dosages of Durian Mesocarpium Ethanol Extracts (EEMD) were divided into six experimental groups to determine the LD50 there are K-(NaCMC); P1 (100 mg/KgBW); P2 (200 mg/KgBW); P3 (400 mg/KgBW); P4 (800 mg/KgBW); P5 (1600 mg/KgBW). The result showed that Durian Mesocarpium Ethanol Extracts (EEMD) has LD50 400 mg/KgBW which included in moderate toxicity with effect like tremor which is one of type acute toxicity due to healthy mice. Conclusion: Based on these results, Durian (Durio zibethinus Linn.) have moderate toxicity after 24 hours oral administration dosages.

Acute Toxicity Screening of Katuk Leaf Extract (Breynia androgyna (L.) Chakrab. & N.P.Balakr) in Mice (Mus musculus) Using the Thompson and Weil Methods

“…The LD50 value was determined by counting the number of deaths of animals for 14 days post-treatment with the stipulated doses. 17 , 18 The Thompson & Weil Method was used to determine the LD50, as shown in the formula below:…”