Acute viral gastroenteritis

Kapikian, Albert Z.

doi:10.1016/0091-7435(74)90019-x

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…In control incubations PBS was used in place of serum. Similar results were obtained by using the "macromethod" described by, Kapikian et al (13).…”

Section: M Mu Noelectron Microscopysupporting

confidence: 88%

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Rotavirus infections in adults in association with acute gastroenteritis

Bonsdorff

Hovi

Mäkelä

et al. 1978

Journal of Medical Virology

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During an epidemic of acute gastroenteritis in Helsinki, in March--May 1976, 18 out of 40 adult patients showed electron microscopic and/or serologic evidence for rotavirus infection. Rotavirus was most frequently seen in the fecal suspensions from 2 to 6 days after the onset of the symptoms but persisted in one patient for as long as 10 days. An increase in the complement-fixing (CF) serum antibody titers against the related Nebraska calf diarrhea virus (NCDV), or an initially high titer and subsequent significant decrease, was seen in all but one patient with rotavirus-positive feces, and in 6 additional patients. This suggests that using electron microscopy as the only diagnostic procedure a considerable number of rotavirus infections in adults remain undetected. Immune response against autologous or homologous rotavirus was also documented by immunoelectron microscopy. Complement-fixing antibody titers against NCDV decreased significantly from the convalescence values over a half-year observation period, but still remained clearly above the titers of a gastroenteritis-negative control population.

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“…In control incubations PBS was used in place of serum. Similar results were obtained by using the "macromethod" described by, Kapikian et al (13).…”

Section: M Mu Noelectron Microscopysupporting

confidence: 88%

“…Ten microliters of the antigen and 10 p1 of heated serum dilution were mixed and incubated for 30 minutes at room temperature. An aliquot of the mixture was then examined in the electron microscope as described above (12,13). In control incubations PBS was used in place of serum.…”

Section: M Mu Noelectron Microscopymentioning

confidence: 99%