Acyl-Lipid Metabolism

Li‐Beisson, Yonghua; Shorrosh, Basil S.; Beisson, Fred; Andersson, Mats X.; Arondel, Vincent; Bates, Philip D.; Baud, Sébastien; Bird, David A.; DeBono, Allan; Durrett, Timothy P.; Franke, Rochus; Graham, Ian A.; Katayama, Kenta; Kelly, Amélie A.; Larson, Tony R.; Markham, Jonathan E.; Miquel, Martine; Molina, Isabel; Nishida, Ikuo; Rowland, Owen; Samuels, Lacey; Schmid, Katherine M.; Wada, Hajime; Welti, Ruth; Xu, Changcheng; Zallot, Rémi; Ohlrogge, John B.

doi:10.1199/tab.0161

Cited by 1,088 publications

(1,032 citation statements)

References 471 publications

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“…The enrichment of the 'suberin biosynthesis' GO term is also consistent with the enrichment in 'fatty acid biosynthesis' given that suberin and associated wax biosynthesis requires de novo fatty acid synthesis (Li-Beisson et al 2013). The induction in cork of several genes related to the synthesis, export from the plastid and elongation of fatty acids (Table S6A), according to the Aralip website (http:// aralip.plantbiology.msu.edu/pathways/pathways), reinforces the involvement of all these pathways in suberin and waxes biosynthesis.…”

Section: Discussionsupporting

confidence: 66%

“…The induction in cork of several genes related to the synthesis, export from the plastid and elongation of fatty acids (Table S6A), according to the Aralip website (http:// aralip.plantbiology.msu.edu/pathways/pathways), reinforces the involvement of all these pathways in suberin and waxes biosynthesis. Similarly, the GO terms 'glycolytic process', 'pyruvate metabolism' and 'lactate metabolic process' are also enriched in cork, probably because genes belonging to these functional categories are required to supply acetylCoA groups that are necessary for the fatty acid synthesis and elongation (Li-Beisson et al 2013). Some genes related to these categories, such as the putative orthologs of Arabidopsis sucrose synthase SUS4 and the plastidial pyruvate kinase PKp1, are clearly upregulated in the cork oak outer bark (Table S6B).…”

Section: Discussionmentioning

confidence: 99%

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A comparative transcriptomic approach to understanding the formation of cork

et al. 2017

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Key message The transcriptome comparison of two oak species reveals possible candidates accounting for the exceptionally thick and pure cork oak phellem, such as those involved in secondary metabolism and phellogen activity. Abstract Cork oak, Quercus suber, differs from other Mediterranean oaks such as holm oak (Quercus ilex) by the thickness and organization of the external bark. While holm oak outer bark contains sequential periderms interspersed with dead secondary phloem (rhytidome), the cork oak outer bark only contains thick layers of phellem (cork rings) that accumulate until reaching a thickness that allows industrial uses. Here we compare the cork oak outer bark transcriptome with that of holm oak. Both transcriptomes present similitudes in their complexity, but whereas cork oak external bark is enriched with upregulated genes related to suberin, which is the main polymer responsible for the protective function of periderm, the upregulated categories of holm oak are enriched in abiotic stress and chromatin assembly. Concomitantly with the upregulation of suberin-related genes, there is also induction of regulatory and meristematic genes, whose predicted activities agree with the increased number of phellem layers found in the cork oak sample. Further transcript profiling among different cork oak tissues and conditions suggests that cork and wood share many regulatory mechanisms, probably reflecting similar ontogeny. Moreover, the analysis of transcripts accumulation during the cork growth season showed that most regulatory genes are upregulated early in the season when the cork cambium becomes active. Altogether our work provides the first transcriptome comparison between cork oak and holm oak outer bark, which unveils new regulatory candidate genes of phellem development.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

A comparative transcriptomic approach to understanding the formation of cork

et al. 2017

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“…One explanation could be that additional lipases are present in photosynthetic tissues, which allow TAG turnover to continue to occur in sdp1. Arabidopsis contains many genes that could potentially have this function (Li-Beisson et al, 2013). CGI58 is one candidate, since the protein has lipase activity (Ghosh et al, 2009) and the mutant accumulates TAG in its leaves to around 0.2% of dry weight (James et al, 2010), which is higher than we detected in sdp1.…”

Section: Discussionmentioning

confidence: 56%

The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis

et al. 2013

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There has been considerable interest recently in the prospect of engineering crops to produce triacylglycerol (TAG) in their vegetative tissues as a means to achieve a step change in oil yield. Here, we show that disruption of TAG hydrolysis in the Arabidopsis (Arabidopsis thaliana) lipase mutant sugar-dependent1 (sdp1) leads to a substantial accumulation of TAG in roots and stems but comparatively much lower TAG accumulation in leaves. TAG content in sdp1 roots increases with the age of the plant and can reach more than 1% of dry weight at maturity, a 50-fold increase over the wild type. TAG accumulation in sdp1 roots requires both ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) and PHOSPHATIDYLCHOLINE: DIACYLGLYCEROL ACYLTRANSFERASE1 and can also be strongly stimulated by the provision of exogenous sugar. In transgenic plants constitutively coexpressing WRINKLED1 and DGAT1, sdp1 also doubles the accumulation of TAG in roots, stems, and leaves, with levels ranging from 5% to 8% of dry weight. Finally, provision of 3% (w/v) exogenous Suc can further boost root TAG content in these transgenic plants to 17% of dry weight. This level of TAG is similar to seed tissues in many plant species and establishes the efficacy of an engineering strategy to produce oil in vegetative tissues that involves simultaneous manipulation of carbohydrate supply, fatty acid synthesis, TAG synthesis, and also TAG breakdown.

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“…Expression levels of lipid metabolism genes were compiled according to the Arabidopsis Acyl-Lipid Metabolism database (http://aralip. plantbiology.msu.edu) (Supplemental Data Set 3) (Beisson et al, 2003;Li-Beisson et al, 2013). In cases where more than one transcript hit the same AGI protein in Arabidopsis, transcript with the highest fragments per kilobase of exon per million fragments value (and presumably highest expression level) was assigned as a representative of the AGI group (Supplemental Data Set 4).…”

Section: Pathway Coordination At the Transcriptome Level In A Lentifmentioning

confidence: 99%

“…Of the identified transcripts, 74,532 were considered as expressed (more than 50 reads per transcript across six samples) (Supplemental Data Set 7), and among the expressed transcripts, 51,230 were successfully annotated. The 1744 transcripts involved in lipid metabolism were categorized based on the lipid gene list (http:// aralip.plantbiology.msu.edu) (Beisson et al, 2003;Li-Beisson et al, 2013) (Supplemental Data Set 8). Transcripts involved in fatty acid synthesis, glycerolipid synthesis, and TAG synthesis were specifically inspected (Supplemental Data Set 9).…”

Section: Rna-seq Analysis Of Wheat Transcriptomementioning

confidence: 99%

Understanding the Biochemical Basis of Temperature-Induced Lipid Pathway Adjustments in Plants

et al. 2015

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Glycerolipid biosynthesis in plants proceeds through two major pathways compartmentalized in the chloroplast and the endoplasmic reticulum (ER). The involvement of glycerolipid pathway interactions in modulating membrane desaturation under temperature stress has been suggested but not fully explored. We profiled glycerolipid changes as well as transcript dynamics under suboptimal temperature conditions in three plant species that are distinctively different in the mode of lipid pathway interactions. In Arabidopsis thaliana, a 16:3 plant, the chloroplast pathway is upregulated in response to low temperature, whereas high temperature promotes the eukaryotic pathway. Operating under a similar mechanistic framework, Atriplex lentiformis at high temperature drastically increases the contribution of the eukaryotic pathway and correspondingly suppresses the prokaryotic pathway, resulting in the switch of lipid profile from 16:3 to 18:3. In wheat (Triticum aestivum), an 18:3 plant, low temperature also influences the channeling of glycerolipids from the ER to chloroplast. Evidence of differential trafficking of diacylglycerol moieties from the ER to chloroplast was uncovered in three plant species as another layer of metabolic adaptation under temperature stress. We propose a model that highlights the predominance and prevalence of lipid pathway interactions in temperature-induced lipid compositional changes.

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Acyl-Lipid Metabolism

Cited by 1,088 publications

References 471 publications

A comparative transcriptomic approach to understanding the formation of cork

A comparative transcriptomic approach to understanding the formation of cork

The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis

Understanding the Biochemical Basis of Temperature-Induced Lipid Pathway Adjustments in Plants

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