Acyl Type of Bacterial Cell Wall: Its Simple Identification by Colorimetric Method

Streptosporangium oxazolinicum sp. nov., a novel endophytic actinomycete producing new antitrypanosomal antibiotics, spoxazomicins

“…28 The N-acyl types of muramic acid were determined by the method of Uchida and Aida. 29 Phospholipids in cells were extracted and identified by the method of Minnikin et al 30 The presence of mycolic acids was examined by TLC following Tomiyasu (1982). 31 Whole-cell sugar composition was analyzed according to the methods of Becker et al 28 Methyl esters of cellular fatty acids were prepared by direct transmethylation with methanolic hydrochloride, and analyzed on a GLC system (HP 6890; Hewlett Packard, Palo Alto, CA, USA).…”

Nocardia veterana sp. nov., isolated from human bronchial lavage.

“…For the analyses of cell wall amino acids and sugars (Stanek & Roberts, 1974), acyl-type of murein (Uchida & Aida, 1977), peptidoglycan and quinone analysis (Minnikin et al, 1984 ;Kroppenstedt, 1982Kroppenstedt, , 1985 and polar lipids (Minnikin et al, 1977), cells were grown in Trypticase soy broth (BBL) for 4 d at 28 mC on a rotary shaker, harvested by centrifugation and washed twice with distilled water. The chemotaxonomic properties of DSM 44445 T were also consistent with their classification into the genus Nocardia (Goodfellow, 1992 ;Goodfellow & Lechevalier, 1989).…”

Isolation of actinomycetes from the root of the plant, Ophiopogon japonicus, and proposal of two new species, Actinoallomurus liliacearum sp. nov. and Actinoallomurus vinaceus sp. nov.

“…The N-acyl types of muramic acid were determined using the method of Uchida and Aida. 25 Phospholipids in cells were extracted and identified using the method of Minnikin et al 26 The presence of mycolic acids was examined by thin layer chromatography in accordance with Tomiyasu. 27 Whole-cell sugar composition was analyzed according to the methods of Becker et al 28 Methyl esters of cellular fatty acids were prepared by direct transmethylation with methanolic hydrochloride, and were analyzed on a GLC system (HP 6890, Hewlett Packard, Palo Alto, CA, USA).…”