Acylsucrose-Producing Tomato Plants Forces Bemisia tabaci to Shift Its Preferred Settling and Feeding Site

Rodríguez-López, Maria; Garzo, Elisa; Bonani, Jean Patrick; Fernández-Muñoz, Rafael; Moriones, Enrique; Fereres, Alberto

doi:10.1371/journal.pone.0033064

Cited by 45 publications

(45 citation statements)

References 35 publications

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“…S5), whiteflies appeared to respond by ovipositing away from the source of 7-epizingiberene production (Fig. 4C) as they do for trichomeproduced acylsugars (31). Also herbivores belonging to different orders were severely affected by 7-epizingiberene.…”

Section: Discussionmentioning

confidence: 83%

Improved herbivore resistance in cultivated tomato with the sesquiterpene biosynthetic pathway from a wild relative

Bleeker

Mirabella

Diergaarde³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

208

192

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Tomato breeding has been tremendously efficient in increasing fruit quality and quantity but did not focus on improving herbivore resistance. The biosynthetic pathway for the production of 7-epizingiberene in a wild tomato was introduced into a cultivated greenhouse variety with the aim to obtain herbivore resistance. 7-Epizingiberene is a specific sesquiterpene with toxic and repellent properties that is produced and stored in glandular trichomes. We identified 7-epizingiberene synthase (ShZIS) that belongs to a new class of sesquiterpene synthases, exclusively using Z-Z-farnesyl-diphosphate (zFPP) in plastids, probably arisen through neo-functionalization of a common ancestor. Expression of the ShZIS and zFPP synthases in the glandular trichomes of cultivated tomato resulted in the production of 7-epizingiberene. These tomatoes gained resistance to several herbivores that are pests of tomato. Hence, introduction of this sesquiterpene biosynthetic pathway into cultivated tomatoes resulted in improved herbivore resistance.metabolic engineering | terpenoid | white fly | spider mite | promoter H erbivorous insects pose serious problems in agricultural production areas. On commercial tomato, whiteflies, spider mites, and aphids are major pests, not only by the feeding damage they cause, but also because they transmit devastating viruses that can cause losses up to 100% (1). Production of tomato, a crop with considerable economic importance, reached 145,751,507 tons worldwide in 2010, representing a value of 53.3 billion dollars (www.fao.org). In general, wild tomatoes are far less attractive to pests than cultivated tomatoes due to an elevated or qualitatively different production of an array of defense compounds such as alkaloids, phenolic compounds, and terpenes.Toxic and repellent compounds are mostly produced in glandular trichomes, autonomous epidermal protrusions specialized in efficient production, storage, and release of defense compounds (2). These glands have long been regarded as economically important and challenging targets for bioengineering (2). Confining metabolic changes to trichomes ensures that the plant metabolism and crop yield remains unaffected.Terpenes constitute the largest and most diverse class of plantproduced secondary compounds that maintain a variety of biological functions and applications, including anticancer (taxol) and antimalarial (artemisin) drugs, in flavor and fragrance industry, and for crop improvement through enhanced pest resistance. Wild tomato sesquiterpenes and derivatives have been implicated in defense against herbivores (3-5). Sesquiterpenes are C 15 -terpenes predominantly derived from precursors of the cytosolic mevalonate pathway where the C 5 -isoprene units isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) are assembled into C 15 E-E-farnesyl diphosphate (FPP) by FPP synthase (FPS) and converted by specific sesquiterpene synthases. However, it was recently shown that some wild tomato species contain a sesquiterpene pathway that is confined to ...

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Section: Discussionmentioning

confidence: 83%

Improved herbivore resistance in cultivated tomato with the sesquiterpene biosynthetic pathway from a wild relative

Bleeker

Mirabella

Diergaarde³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

208

192

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“…Resistance to B. tabaci and its relation to plant morphological traits has been well documented in other crops, such as tomato, cotton, and cassava (Bellotti and Arias, 2001;Boiça et al, 2007;Oriani and Vendramim, 2010). Physical barriers, such as the cuticle thickness of leaf may prevent the insect stylet from reaching the phloem (Janssen et al, 1989), while the presence of high density of glandular trichomes may cause high mortality of whitefly as compounds act as a glued trap for adult whiteflies, in addition, the acylsugars produced by such trichomes deters settling and probing of B. tabaci (Heinz and Zalom, 1995;Liedl et al, 1995;Rodríguez-López et al, 2012). The internal characteristics of the leaves, such as chemical composition of leaf sap, nutritional value of leaves, and activity of plant defense-related enzymes has been also implicated but less studied in host resistance to B. tabaci.…”

Section: Introductionmentioning

confidence: 99%

Morphological characterization of Capsicum annuum L. accessions from southern Mexico and their response to the Bemisia tabaci-Begomovirus complex

Ballina-Gómez¹,

Latournerie-Moreno²,

Ruíz-Sánchez³

et al. 2013

Chilean J. Agric. Res.

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The high diversity of chili pepper (Capsicum annuum L.) in Mexico offers an excellent alternative to search for wild and semi-domesticated genotypes as sources of resistance to the complex Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae)-Begomovirus, which has caused enormous losses in commercial production of various horticultural crops. The goal of the present work was to characterize ex situ 18 genotypes of C. annuum from southern Mexico through 47 morphological descriptors, and to evaluate its response to the B. tabaci-Begomovirus complex. Morphological characterization showed the variables calyx annular constriction (CAC), number of branch bifurcation (NBB), and calyx pigmentation (CP) had the highest variation. Principal components analysis (PCA) of 47 morphological characteristics showed that 12 components were selected as meaningful factors. These components explained 94% of the variation. Cluster analysis showed three major clusters and seven sub-clusters. On the other hand, evaluation of the response to B. tabaci-Begomovirus showed that the genotypes have differential susceptibility to this vector-pathogen complex. Genotypes 'Chawa', 'Blanco', 'Maax' and 'X´catic' were into the low susceptibility to B. tabaci and low severity of viral symptoms. Surprisingly, the genotype 'Simojovel' showed high susceptibility to whitefly, but was grouped into genotypes with low symptom severity. This study shows the potential of native germplasm of pepper to explore sources of resistance to the B. tabaci-Begomovirus complex.

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“…For example, the cuticle thickness of leaf may prevent the insect stylet from reaching the phloem (Firdaus et al, 2011). While the presence of high density of glandular trichomes may cause high mortality of whiteflies, due to the secretion of chemical compounds such as acylsugars, which act as a glued trap for the whiteflies and attractant for natural enemies (Rodríguez et al, 2012). Resistance to B. tabaci and its relation to plant morphological traits has been well documented in tomato, cotton, and cassava (Bellotti and Arias, 2001;Boiça et al, 2007;Oriani and Vendramim, 2010).…”

Section: Introductionmentioning

confidence: 99%

Survival of Bemisia tabaci and activity of plant defense-related enzymes in genotypes of Capsicum annuum L.

Latournerie-Moreno¹,

Ic-Caamal²,

Ruíz-Sánchez³

et al. 2015

Chilean J. Agric. Res.

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The whitefly Bemisia tabaci (Gennadius, 1889) is a major plant pest of horticultural crops from the families Solanaceae, Fabaceae and Cucurbitaceae in Neotropical areas. The exploration of host plant resistance and their biochemical mechanisms offers an excellent alternative to better understand factors affecting the interaction between phytophagous insect and host plant. We evaluated the survival of B. tabaci in landrace genotypes of Capsicum annuum L., and the activity of plant defense-related enzymes (chitinase, polyphenoloxidase, and peroxidase). The landrace genotypes Amaxito, Tabaquero, and Simojovel showed resistance to B. tabaci, as we observed more than 50% nymphal mortality, while in the commercial susceptible genotype Jalapeño mortality of B. tabaci nymphs was not higher than 20%. The activities of plant defense-related enzymes were significantly different among pepper genotypes (P < 0.05). Basal activities of chitinase, polyphenoloxidase and peroxidase were significantly lower or equal in landrace genotypes than that of the commercial genotype Jalapeño. The activity of plant enzymes was differential among pepper genotypes (P < 0.05). For example, the activity of chitinase enzyme generally was higher in non-infested plants with B. tabaci than those infested. Instead polyphenoloxidase ('Amaxito' and 'Simojovel') and peroxidase enzymes activities ('Tabaquero') increased in infested plants (P < 0.05). We conclude that basal activities of plant defense-related enzymes could be act through other mechanism plant induction, since plant defense-related enzymes showed a different induction response to B. tabaci. We underlined the role of polyphenoloxidase as plant defense in the pepper genotype Simojovel related to B. tabaci.

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Acylsucrose-Producing Tomato Plants Forces Bemisia tabaci to Shift Its Preferred Settling and Feeding Site

Cited by 45 publications

References 35 publications

Improved herbivore resistance in cultivated tomato with the sesquiterpene biosynthetic pathway from a wild relative

Improved herbivore resistance in cultivated tomato with the sesquiterpene biosynthetic pathway from a wild relative

Morphological characterization of Capsicum annuum L. accessions from southern Mexico and their response to the Bemisia tabaci-Begomovirus complex

Survival of Bemisia tabaci and activity of plant defense-related enzymes in genotypes of Capsicum annuum L.

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