Ad-Dressing Stem Cells: Hydrogels for Encapsulation

Kandilogiannakis, Leonidas; Filidou, Eirini; Kolios, George; Paspaliaris, Vasilis

doi:10.3390/pr9010011

Cited by 7 publications

(8 citation statements)

References 156 publications

(215 reference statements)

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“…In the search for the most suitable biomaterial for cell microencapsulation, synthetic polymers (e.g., poly(ethylene glycol) (PEG) [ 12 , 13 ], polyurethane [ 14 ], polyvinyl alcohol (PVA) [ 15 ]) have been extensively explored. Although microcapsules can be shaped with these materials through different methods, most microencapsulation systems rely on natural polymer-based hydrogels, such as alginate [ 16 – 18 ] and hyaluronic acid [ 19 , 20 ], due to their high water content and consistency mimicking the physical features of the extracellular matrix (ECM) [ 21 , 22 ]. For instance, alginate has been extensively employed in microencapsulation approaches because it is biocompatible, widely available, and easily cross-linked in the presence of divalent cations, such as Ca 2+ .…”

Section: Introductionmentioning

confidence: 99%

Silk fibroin microgels as a platform for cell microencapsulation

Bono

Saroglia

Marcuzzo

et al. 2022

J Mater Sci: Mater Med

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Cell microencapsulation has been utilized for years as a means of cell shielding from the external environment while facilitating the transport of gases, general metabolites, and secretory bioactive molecules at once. In this light, hydrogels may support the structural integrity and functionality of encapsulated biologics whereas ensuring cell viability and function and releasing potential therapeutic factors once in situ. In this work, we describe a straightforward strategy to fabricate silk fibroin (SF) microgels (µgels) and encapsulate cells into them. SF µgels (size ≈ 200 µm) were obtained through ultrasonication-induced gelation of SF in a water-oil emulsion phase. A thorough physicochemical (SEM analysis, and FT-IR) and mechanical (microindentation tests) characterization of SF µgels were carried out to assess their nanostructure, porosity, and stiffness. SF µgels were used to encapsulate and culture L929 and primary myoblasts. Interestingly, SF µgels showed a selective release of relatively small proteins (e.g., VEGF, molecular weight, MW = 40 kDa) by the encapsulated primary myoblasts, while bigger (macro)molecules (MW = 160 kDa) were hampered to diffusing through the µgels. This article provided the groundwork to expand the use of SF hydrogels into a versatile platform for encapsulating relevant cells able to release paracrine factors potentially regulating tissue and/or organ functions, thus promoting their regeneration. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Silk fibroin microgels as a platform for cell microencapsulation

Bono

Saroglia

Marcuzzo

et al. 2022

J Mater Sci: Mater Med

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“…Mechanical manipulation such as syringe injection, cryopreservation, and thawing are some of the fundamental features in stem cell therapy, which allows on-demand and off-the-shelf clinical application [40]. We cryopreserved the biosynspheres for 6 months, thawed, and then extrude the biosynspheres as a simulation of clinical manipulation.…”

Section: Discussionmentioning

confidence: 99%

A clinical feasible stem cell encapsulation ensures an improved wound healing

Zuo

Lü²,

Zhang³

et al. 2023

Biomed. Mater.

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Cell encapsulation has proven to be promising in stem cell therapy. However, there are issues needed to be addressed, including unsatisfied yield, unmet clinically friendly formulation, and unacceptable viability of stem cells after cryopreservation and thawing. We developed a novel biosynsphere technology to encapsulate stem cells in clinically-ready biomaterials with controlled microsphere size. We demonstrated that biosynspheres ensure the bioviability and functionality of adipose-derived stromal cells (ADSCs) encapsulated, as delineated by a series of testing procedures. We further demonstrated that biosynspheres protect ADSCs from the hardness of clinically handling such as cryopreservation, thawing, high-speed centrifugation and syringe/nozzle injection. In a swine full skin defect model, we showed that biosynspheres were integrated to the destined tissues and promoted the repair of injured tissues with an accelerating healing process, less scar tissue formation and normalized deposition of collagen type I and type III, the ratio similar to that found in normal skin. These findings underscore the potential of biosynsphere as an improved biofabrication technology for tissue regeneration in clinical setting.

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“…NSCs also contribute to the strengthening of recipient neuronal axons ( Assunção-Silva et al, 2015 ). In addition to the fact that NSCs (encapsulated) within the matrices in injured SC differentiate into neurons and oligodendrocytes, they are also able to migrate in the injury site, enhance neuronal regeneration and reduce inflammatory processes in SC ( Skop et al, 2014 ; Kandilogiannakis et al, 2021 ).…”

Section: Advances Of Hydrogel Combined With Neural Stem Cells In Scimentioning

confidence: 99%

Modern advances in spinal cord regeneration: hydrogel combined with neural stem cells

Rybachuk,

Nesterenko,

Zhovannyk

2024

Front. Pharmacol.

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Severe spinal cord injuries (SCI) lead to loss of functional activity of the body below the injury site, affect a person’s ability to self-care and have a direct impact on performance. Due to the structural features and functional role of the spinal cord in the body, the consequences of SCI cannot be completely overcome at the expense of endogenous regenerative potential and, developing over time, lead to severe complications years after injury. Thus, the primary task of this type of injury treatment is to create artificial conditions for the regenerative growth of damaged nerve fibers through the area of the SCI. Solving this problem is possible using tissue neuroengineering involving the technology of replacing the natural tissue environment with synthetic matrices (for example, hydrogels) in combination with stem cells, in particular, neural/progenitor stem cells (NSPCs). This approach can provide maximum stimulation and support for the regenerative growth of axons of damaged neurons and their myelination. In this review, we consider the currently available options for improving the condition after SCI (use of NSC transplantation or/and replacement of the damaged area of the SCI with a matrix, specifically a hydrogel). We emphasise the expediency and effectiveness of the hydrogel matrix + NSCs complex system used for the reconstruction of spinal cord tissue after injury. Since such a complex approach (a combination of tissue engineering and cell therapy), in our opinion, allows not only to creation of conditions for supporting endogenous regeneration or mechanical reconstruction of the spinal cord, but also to strengthen endogenous regeneration, prevent the spread of the inflammatory process, and promote the restoration of lost reflex, motor and sensory functions of the injured area of spinal cord.

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Ad-Dressing Stem Cells: Hydrogels for Encapsulation

Cited by 7 publications

References 156 publications

Silk fibroin microgels as a platform for cell microencapsulation

Silk fibroin microgels as a platform for cell microencapsulation

A clinical feasible stem cell encapsulation ensures an improved wound healing

Modern advances in spinal cord regeneration: hydrogel combined with neural stem cells

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