ADAM10 localization in temporomandibular joint disk with internal derangement: an ex vivo immunohistochemical study

Abstract

Abstract:The purpose of this study was to determine the presence of ADAM10 in temporomandibular joint disk with internal derangement. Twentyfive paraffin blocks of displaced temporomandibular joint (TMJ) disk specimens from earlier investigations were retrieved from the archives of the University of Catania. Of these 16 had been removed from females and 9 from males; 11 with anterior disk displacement with reduction (ADDwR) and 14 with anterior disk displacement without reduction (ADDwoR). The sections were dehydrated, embedded in paraffin and cut. Then they were incubated in 0.3% H2O2/methanol and half of sections from each sample were incubated in diluted rabbit polyclonal anti-ADAM10 antibody. Then biotinylated antimouse/anti-rabbit IgG was applied to the sections, followed by avidin-biotinperioxidase complex. The results were analyzed and the results were that ADAM10 was overexpressed in the posterior band of sections from patients with ADDwR compared to the other bands of both ADDwR and ADDwoR sections. Overexpression correlated with severe histopathological degeneration. We believe these results have the potential to provide insights into the pathogenesis of TMJ disk degeneration and to help design new therapeutic approaches targeting the proteolytic events that lead to tissue degeneration. Early therapeutic block of ADAM10 activity could succeed in limiting aggrecan-rich matrix breakdown without affecting normal physiology.

Neuron/Glial Antigen 2-Type VI Collagen Interactions During Murine Temporomandibular Joint Osteoarthritis

“…NG2 in secondary cartilages such as the TMJ has never been characterized. NG2 has promise as a critical regulator of TMJ cartilage homeostasis because of a pericellular matrix rich in type VI collagen 7 , the presence of the NG2 ligands FGF2 and PDGF 27,28 , and the elevated levels of NG2 active proteases MMP13, MMP14, and ADAM10 during TMJ OA 29,30 . The goal of this study was to evaluate and define the interaction of the NG2 ectodomain with type VI collagen during the progression of TMJ OA and to evaluate if NG2 internalization is contextually linked with the inflammatory and/or mechanical stresses associated with TMJ OA.…”

Immunohistochemical panel of degenerated articular discs from patients with temporomandibular joint osteoarthritis

“…In such conditions, cartilage turnover keeps the disc healthy 19,20 . Otherwise, joint overloading may stimulate catabolic cytokine expression, inducing matrix degradation and compromising proteoglycan and glycosaminoglycan synthesis, mainly by action of metalloproteinases (MMPs) 12 . Although disc displacement has been regarded as a result of these changes rather than the cause for OA, Fazaeli et al 20 stated that even slight displacements can lead to unfavourable biomechanical circumstances 2,14 .…”

“…Until recently, immunohistochemical assays on human TMJ discs were based on subjective assessments of biomarkers' expression. Computerised histomorphometry of TMJ discs has been applied for the investigation of internal derangement and seldom for TMJOA 12,32,48 . We determined both immunohistochemistry results and collagen degeneration through histomorphometric image analysis in an effort to strengthen the validity of results and allow reproducibility.…”

“…The TMJ disc is a fibrocartilage tissue containing fibroblasts, fibrochondrocytes and an extracellular matrix (ECM) composed mainly from type I collagen and proteoglycan complexes 11,12 . It presents as a biconcave anatomical structure and may be divided into three different regions along its anteroposterior axis: anterior band (AB), intermediate zone (IZ) and posterior band (PB).…”

Shear Wave Elastography in the Evaluation of Temporomandibular Joint Disorders

“…The evidence from other studies also suggests that the articular disc in TMD patients might undergo a proteolytic remodeling. One study reported that a TMD was associated with an overexpression of ADAM10 protease in the posterior bands of the disc, which resulted in an enhanced breakdown of the aggrecan-rich matrix (Loreto et al 2016). In another study, the immunoreactivity of ADAMTS-4, a metalloproteinase responsible for proteoglycan degradation, was found in the anterior bands of the disc (Leonardi et al 2015).…”