ADAMTS1 and HSPG2 mRNA levels in cumulus cells are related to human oocyte quality and controlled ovarian hyperstimulation outcomes

Abstract

Purpose The study investigated potential correlations between the expression levels of ADAMTS1 and HSPG2 in cumulus cells (CCs) and controlled ovarian hyperstimulation (COH) outcomes. Methods RT-PCR was used to determine ADAMTS1 and HSPG2 mRNA levels in mice CCs at different timepoints (0, 4, 8, 12, and 16 h) after human chorionic gonadotropin (hCG) injection, and in CCs after RNAi treatment. Women with polycystic ovary syndrome (PCOS) (n = 45) and normal ovulatory controls (n = 103) undergoing IVF/ICSI were recruited. Relative ADAMTS1 and HSPG2 mRNA levels were measured by RT-PCR. Moreover, correlations of ADAMTS1 and HSPG2 levels with COH outcomes were analyzed. Results At different timepoints after hCG treatment, ADAMTS1 mRNA had the highest level at 12 h, whereas HSPG2 showed opposite profiles to ADAMTS1 with the lowest level at 12 h. HSPG2 expression was upregulated after ADAMTS1 RNAi treatment The PCOS group had higher HSPG2 and lower ADAMTS1 expression levels than controls. In normal ovulatory women (control group), a higher expression of ADAMTS1 and lower expression of HSPG2 were associated with more mature oocytes, transplantable embryos, and good quality embryos, whereas higher transplantable embryo rates and good quality embryo rates were obtained only with lower HSPG2 expression. ROC curves showed the co-measurement of ADAMTS1 and HSPG2 had a better predictive power than separate analyses. Conclusion The dynamic profiles of ADAMTS1 and HSPG2 were inversely correlated in CCs. In PCOS and normal ovulatory patients, higher ADAMTS1 and lower HSPG2 expression levels in CCs were related to better COH outcomes.

Screening target genes for the treatment of PCOS via analysis of single-cell sequencing data

“…ADAMTS1 was reported relevant to premature ovarian failure and menopause and to be significantly increased in granulosa cells in PCOS [ 31 ]. ADAMTS1 expression was found to exhibit a positive correlation with oocyte fertilisation rate [ 32 ] and more mature oocytes, transplantable embryos and better-quality embryos [ 33 ]; however, we found it to be significantly down regulated in our analysis. via the screening of drugs relevant to these 3 DEGs, we found that most therapeutic drugs targeted PGR and the majority of these were progesterone drugs, exerting both progestogenic and anti-androgenic effects.…”

Sohlh1 and Lhx8 are prominent biomarkers to estimate the primordial follicle pool in mice

“…The thermal cycler conditions were as follows: 30 s at 95.0 °C for cDNA denatured, followed by 40 cycles of 15 s at 95 °C and 60 °C for the 34 s. Verification of specific product amplification was performed by dissociation curve analysis. The mRNA relative expression was calculated by the 2^ −ΔCt method with Gapdh used as an internal control [ 16 , 17 ].Primer sequences for each gene were listed in Supplementary Table 1 .…”

Genetic architecture of non‐syndromic skeletal class III malocclusion

“…As a member of the ADAMTS protein family, ADAMTS1 is involved in many biological processes, including catalytic activity against proteoglycans, and the degradation of collagen (Ma et al, 2020). The SNPs rs2738 and rs229038 of the ADAMTS1 gene were reported to be associated with MP in 230 unrelated patients and 196 unrelated controls (Guan et al, 2015).…”