ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries

Kaufmann, Jan; Brangsch, Julia; Kader, Avan; Saatz, Jessica; Mangarova, Dilyana B.; Zacharias, Martin; Kempf, W.; Schwaar, Timm; Ponader, Marco; Adams, Lisa C.; Möckel, Jana; Botnar, René M.; Taupitz, Matthias; Mägdefessel, Lars; Traub, Heike; Hamm, Bernd; Weller, Michael G.; Makowski, Marcus R.

doi:10.1038/s41467-022-30464-8

Cited by 8 publications

(7 citation statements)

References 66 publications

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“…The possible association of ILT on histologic appearance should be investigated in a larger patient cohort. In this context, experimental radiology using new radioactive tracers or specific magnetic resonance imaging probes have shown an unequal distribution of their respective target in human aortic aneurysm, both in circumferent and longitudinal direction [ 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

Completing the view – histologic insights from circular AAA specimen including 3D imaging

Menges,

Nackenhorst,

Müller

et al. 2023

Diagn Pathol

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Abdominal aortic aneurysm (AAA) is a pathologic enlargement of the infrarenal aorta with an associated risk of rupture. However, the responsible mechanisms are only partially understood. Based on murine and human samples, a heterogeneous distribution of characteristic pathologic features across the aneurysm circumference is expected. Yet, complete histologic workup of the aneurysm sac is scarcely reported. Here, samples from five AAAs covering the complete circumference partially as aortic rings are investigated by histologic means (HE, EvG, immunohistochemistry) and a new method embedding the complete ring. Additionally, two different methods of serial histologic section alignment are applied to create a 3D view. The typical histopathologic features of AAA, elastic fiber degradation, matrix remodeling with collagen deposition, calcification, inflammatory cell infiltration and thrombus coverage were distributed without recognizable pattern across the aneurysm sac in all five patients. Analysis of digitally scanned entire aortic rings facilitates the visualization of these observations. Immunohistochemistry is feasible in such specimen, however, tricky due to tissue disintegration. 3D image stacks were created using open-source and non-generic software correcting for non-rigid warping between consecutive sections. Secondly, 3D image viewers allowed visualization of in-depth changes of the investigated pathologic hallmarks. In conclusion, this exploratory descriptive study demonstrates a heterogeneous histomorphology around the AAA circumference. Warranting an increased sample size, these results might need to be considered in future mechanistic research, especially in reference to intraluminal thrombus coverage. 3D histology of such circular specimen could be a valuable visualization tool for further analysis.

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Section: Discussionmentioning

confidence: 99%

Completing the view – histologic insights from circular AAA specimen including 3D imaging

Menges,

Nackenhorst,

Müller

et al. 2023

Diagn Pathol

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show abstract

“… 25 , 26 Thus, new imaging methods using radioactive or molecular magnetic resonance imaging probes are currently evaluated on a preclinical level to combine histologic features of remodeling with in vivo imaging approaches. 27 , 28 , 29 Ideally, such data will be considered in future versions of FEM-based AAA biomechanics to integrate remodeling during aneurysm growth and increase the precision of the rupture risk assessment.…”

Section: Discussionmentioning

confidence: 99%

Rupture risk parameters upon biomechanical analysis independently change from vessel geometry during abdominal aortic aneurysm growth

Zschäpitz

Bohmann

Lutz

et al. 2023

JVS-Vascular Science

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“…The library contained glycine, serine, proline, tyrosine, glutamine, isoleucine, phenylalanine, tryptophane, histidine, glutamic acid, and arginine to cover hydrophilic, hydrophobic, basic, acidic, and aromatic amino acids. Similar amino acid compositions have previously proved suitable for the generation of peptide libraries [ 45 ]. The beads were immobilized on an electrically conductive, double-sided adhesive tape attached to a glass microscope slide.…”

Section: Methodsmentioning

confidence: 99%

Efficient Purification of Cowpea Chlorotic Mottle Virus by a Novel Peptide Aptamer

Tscheuschner

Ponader

Raab

et al. 2023

Viruses

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The cowpea chlorotic mottle virus (CCMV) is a plant virus explored as a nanotechnological platform. The robust self-assembly mechanism of its capsid protein allows for drug encapsulation and targeted delivery. Additionally, the capsid nanoparticle can be used as a programmable platform to display different molecular moieties. In view of future applications, efficient production and purification of plant viruses are key steps. In established protocols, the need for ultracentrifugation is a significant limitation due to cost, difficult scalability, and safety issues. In addition, the purity of the final virus isolate often remains unclear. Here, an advanced protocol for the purification of the CCMV from infected plant tissue was developed, focusing on efficiency, economy, and final purity. The protocol involves precipitation with PEG 8000, followed by affinity extraction using a novel peptide aptamer. The efficiency of the protocol was validated using size exclusion chromatography, MALDI-TOF mass spectrometry, reversed-phase HPLC, and sandwich immunoassay. Furthermore, it was demonstrated that the final eluate of the affinity column is of exceptional purity (98.4%) determined by HPLC and detection at 220 nm. The scale-up of our proposed method seems to be straightforward, which opens the way to the large-scale production of such nanomaterials. This highly improved protocol may facilitate the use and implementation of plant viruses as nanotechnological platforms for in vitro and in vivo applications.

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ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries

Cited by 8 publications

References 66 publications

Completing the view – histologic insights from circular AAA specimen including 3D imaging

Completing the view – histologic insights from circular AAA specimen including 3D imaging

Rupture risk parameters upon biomechanical analysis independently change from vessel geometry during abdominal aortic aneurysm growth

Efficient Purification of Cowpea Chlorotic Mottle Virus by a Novel Peptide Aptamer

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