ADaphnia magnaFirst-Brood Chronic Test: An Alternative to the Conventional 21-Day Chronic Bioassay?

Guilhermino, Lúcia; Sobral, Olímpia; Chastinet, Carla B.A.; Ribeiro, Rui; Gonçalves, Fernando; Silva, Carolina; Soares, Amadeu M.V.M.

doi:10.1006/eesa.1998.1730

Cited by 54 publications

(21 citation statements)

References 20 publications

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“…Being so, removal of eggs from the brood chamber would enhance their exposure to the toxicant and, thus, signi"cant e!ects would be anticipated at lower 3,4-DCA concentrations. Indeed, such increased sensitivity was found in the present study: the LOEC value for the duration of the "rst egg stage (0.625 g L\) was much lower than LOECs reported in the literature for the D. magna reproduction test: 5}20 (Soares et al, 1990), 10 (Diamantino et al, 1997, 12 (Elendt, 1990), and (Guilhermino et al, 1999) 20 g L\.…”

Section: Toxicity Testingcontrasting

confidence: 79%

“…Baird et al (1991b) pointed out that this toxicant a!ects embryonic development, inside the brood chamber, more than egg production in female ovaries. Guilhermino et al (1999) showed that it was possible to reduce chronic exposure to 3,4-DCA, from 21 to 10 days, with a similar "nal EC for D. magna reproduction. Being so, removal of eggs from the brood chamber would enhance their exposure to the toxicant and, thus, signi"cant e!ects would be anticipated at lower 3,4-DCA concentrations.…”

Section: Toxicity Testingmentioning

confidence: 97%

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In Vitro Development of Parthenogenetic Eggs: A Fast Ecotoxicity Test with Daphnia magna?

Sobral

Chastinet

Nogueira

et al. 2001

Ecotoxicology and Environmental Safety

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In the present study, the authors investigated and defined development stages of Daphnia magna eggs that could be potential endpoints for sublethal toxicity tests with in vitro parthenogenetic egg cultures. Such an "egg test" could be a cost-effective alternative to the classic 21-day test with D. magna. Three main stages of embryonic development were considered: release of the egg external membrane, release of the internal membrane, and separation of the caudal spine. The first embryonic stage was attained approximately 30 h after transfer of eggs from ovaries to the brood chamber (considered as time zero), the second stage at 48 h, and the third stage at 68 h. Embryonic development was considered completed with the caudal spine separation. Thereafter, juveniles were able to swim in the water column. Egg mortality, duration of each egg stage, egg diameter, and egg abnormalities were investigated as potential endpoints. In vitro tests were carried out with several toxicants (DBS, 3,4-DCA, cadmium, and copper) and with acid mine drainage, sensitivity generally being higher than with the accepted chronic 21-day test with D. magna.

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Section: Toxicity Testingcontrasting

confidence: 79%

Section: Toxicity Testingmentioning

confidence: 97%

In Vitro Development of Parthenogenetic Eggs: A Fast Ecotoxicity Test with Daphnia magna?

Sobral

Chastinet

Nogueira

et al. 2001

Ecotoxicology and Environmental Safety

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“…A positive association between the number of aborted eggs and the testosterone dose was observed suggesting that this compound reduced the reproduction of daphnids by interfering with the normal eggs development. Several authors (Baird et al, 1991;Guilhermino et al, 1999) verified that sodium bromide and 3,4-dichloroaniline (DCA) caused greater abnormal embryonic development, an increased level of abortion and reduced the total number of viable offspring released. In addition, the egg production was unaffected by chronic exposure to both chemical, which conforms to D. magna behavior (Baird et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Acute and chronic effects of testosterone and 4-hydroxyandrostenedione to the crustacean Daphnia magna

Barbosa

Nogueira

Soares

2008

Ecotoxicology and Environmental Safety

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a b s t r a c tSteroid compounds have been globally detected in surface waters. The ecological impacts of these biologically active chemicals are largely unknown. Toxicity of testosterone and 4-hydroxyandrostenedione was assessed for the freshwater cladoceran Daphnia magna. Acute toxicity tests showed that 6.20 mg L À1 of testosterone, the highest concentration tested, did not have effect on the daphnids, whereas 4-hydroxyandrostenedione had an EC 50 of 4.26 mg L À1 . Chronic toxicity tests were carried out using survival, body length, fecundity, and fertility as endpoints. Long-term testosterone exposure reduced D. magna fecundity and fertility at concentrations ranging from 0.31 to 2.48 mg L À1 . The significant decrease in fecundity was associated with an increase in aborted eggs. Long-term 4-hydroxyandrostenedione exposure at 0.84 mg L À1 increased the mortality of the neonates. The chronic toxicity effects were observed at concentrations higher than the measured environmental concentrations of these compounds. Nevertheless, the reproductive impairment of the daphnids is likely to occur at environmental levels as an ultimate response to long-term exposure.

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“…The number of the eggs and the young worms, which are continuous data, were "tted by a sigmoid curve. The logistic model was obtained by a more general model largely used in pharmacology to detect dose}response e!ects (Neter et al, 1985;Silva et al, 1995) and more recently also used in ecotoxicology (OECD, 1995;Field et al, 1999;Guilhermino et al, 1999). …”

Section: Discussionmentioning

confidence: 99%

“…The general model, which is an extension of the model for binary responses, is The resulting model used is: The EC was estimated because it is considered to be particularly appropriate to evalute the NOEC (Guilhermino et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Toxicity of 4-Nonylphenol to Tubifex tubifex and Chironomus riparius in 28-Day Whole-Sediment Tests

Bettinetti

Provini

2002

Ecotoxicology and Environmental Safety

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ADaphnia magnaFirst-Brood Chronic Test: An Alternative to the Conventional 21-Day Chronic Bioassay?

Cited by 54 publications

References 20 publications

In Vitro Development of Parthenogenetic Eggs: A Fast Ecotoxicity Test with Daphnia magna?

In Vitro Development of Parthenogenetic Eggs: A Fast Ecotoxicity Test with Daphnia magna?

Acute and chronic effects of testosterone and 4-hydroxyandrostenedione to the crustacean Daphnia magna

Toxicity of 4-Nonylphenol to Tubifex tubifex and Chironomus riparius in 28-Day Whole-Sediment Tests

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