Adaptation following intestinal resection: mechanisms and signals

Thiesen, Aducio; Drozdowski, Laurie; Iordache, Claudiu; Neo, C.C; Woudstra, Trudy D.; Xenodemetropoulos, Ted; Keelan, M.; Clandinin, M. T.; Thomson, A. B. R.; Wild, Gary

doi:10.1016/s1521-6918(03)00097-0

Cited by 39 publications

(18 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In fact, whereas alterations in proliferation and migration of enterocytes and in mucosal surface area are considered non-specific adaptive changes, those in density of transporters are considered specific [32][33][34]. In fact, whereas alterations in proliferation and migration of enterocytes and in mucosal surface area are considered non-specific adaptive changes, those in density of transporters are considered specific [32][33][34].…”

Section: Discussionmentioning

confidence: 99%

Maternal protein restriction during pregnancy affects gene expression and immunolocalization of intestinal nutrient transporters in rats

Pinheiro

Buratini

et al. 2013

Clinical Science

View full text Add to dashboard Cite

Intrauterine dietary restriction may cause changes in the functioning of offspring organs and systems later in life, an effect known as fetal programming. The present study evaluated mRNA abundance and immunolocalization of nutrient transporters as well as enterocytes proliferation in the proximal, median and distal segments of small intestine of rats born to protein-restricted dams. Pregnant rats were fed hypoproteic (6% protein) or control (17% protein) diets, and offspring rats were evaluated at 3 and 16 weeks of age. The presence of SGLT1 (sodium-glucose co-transporter 1), GLUT2 (glucose transporter 2), PEPT1 (peptide transporter 1) and the intestinal proliferation were evaluated by immunohistochemical techniques and the abundance of specific mRNA for SGLT1, GLUT2 and PEPT1 was assessed by the real-time PCR technique. Rats born to protein-restricted dams showed higher cell proliferation in all intestinal segments and higher gene expression of SGLT1 and PEPT1 in the duodenum. Moreover, in adult animals born to protein-restricted dams the immunoreactivity of SGLT1, GLUT2 and PEPT1 in the duodenum was more intense than in control rats. Taken together, the results indicate that changes in the small intestine observed in adulthood can be programmed during the gestation. In addition, they show that this response is caused by both up-regulation in transporter gene expression, a specific adaptation mechanism, and intestinal proliferation, an unspecific adaptation mechanism.

show abstract

Section: Discussionmentioning

confidence: 99%

Maternal protein restriction during pregnancy affects gene expression and immunolocalization of intestinal nutrient transporters in rats

Pinheiro

Buratini

et al. 2013

Clinical Science

View full text Add to dashboard Cite

show abstract

“…In the present study, a mouse model of ICR previously developed by Dekaney et al [24] was used to determine the impact of ICR on the microbiota in murine jejunum and colon. Other commonly used resection models include proximal small bowel resection in rat, pig or mouse models [25] but we developed the ICR model since ICR is a more frequent surgery in humans than proximal small bowel resection. An ICR model has also been developed in rats [26] but a mouse model has the potential advantage that it can be applied to genetically manipulated mice that develop spontaneous gastrointestinal diseases, such as IBD models [27].…”

Section: Introductionmentioning

confidence: 99%

Impact of Ileocecal Resection and Concomitant Antibiotics on the Microbiome of the Murine Jejunum and Colon

et al. 2013

View full text Add to dashboard Cite

Ileocecal resection (ICR) is a commonly required surgical intervention in unmanageable Crohn’s disease and necrotizing enterocolitis. However, the impact of ICR, and the concomitant doses of antibiotic routinely given with ICR, on the intestinal commensal microbiota has not been determined. In this study, wild-type C57BL6 mice were subjected to ICR and concomitant single intraperitoneal antibiotic injection. Intestinal lumen contents were collected from jejunum and colon at 7, 14, and 28 days after resection and compared to non-ICR controls. Samples were analyzed by16S rRNA gene pyrosequencing and quantitative PCR. The intestinal microbiota was altered by 7 days after ICR and accompanying antibiotic treatment, with decreased diversity in the colon. Phylogenetic diversity (PD) decreased from 11.8 ± 1.8 in non-ICR controls to 5.9 ± 0.5 in 7-day post-ICR samples. There were also minor effects in the jejunum where PD values decreased from 8.3 ± 0.4 to 7.5 ± 1.4. PCoA analysis indicated that bacterial populations 28 days post-ICR differed significantly from non-ICR controls. Moreover, colon and jejunum bacterial populations were remarkably similar 28 days after resection, whereas the initial communities differed markedly. Firmicutes and Bacteroidetes were the predominant phyla in jejunum and colon before ICR; however, Firmicutes became the vastly predominant phylum in jejunum and colon 28 days after ICR. Although the microbiota returned towards a homeostatic state, with re-establishment of Firmicutes as the predominant phylum, we did not detect Bacteroidetes in the colon 28 days after ICR. In the jejunum Bacteroidetes was detected at a 0.01% abundance after this time period. The changes in jejunal and colonic microbiota induced by ICR and concomitant antibiotic injection may therefore be considered as potential regulators of post-surgical adaptive growth or function, and in a setting of active IBD, potential contributors to post-surgical pathophysiology of disease recurrence.

show abstract

“…Although tissue collagen contents are not involved among intestinal adaptation parameters, and the data regarding the non-mucosal changes following massive small bowel resection is extremely limited, the analysis of soluble collagen fractions is considered a reliable indicator of intestinal wound healing [34,35]. On the other hand, the "glutamate family" of amino acids plays an important role on fibroblastic growth, and thus, tissue collagen production [36].…”

Section: Gln -Glutamine; Gh -Growth Hormon; En -Enteral Nutrition; Hgmentioning

confidence: 99%

The effects of parenteral glutamine on intestinal adaptation in a rat model of short bowel syndrome

et al. 2010

View full text Add to dashboard Cite

AbstractShort bowel syndrome (SBS) is characterized by the malabsorption of nutrients and fluids that occurs after major intestinal resection, resulting from an adaptation process that begins immediately to increase the mucosal surface area and absorption. Certain nutrients and trophic factors are widely used to increase intestinal adaptation following massive intestinal resection. The efficacy and benefits of glutamine on the intestinal adaptation process is still controversial. This study was conducted to determine the effects of parenteral glutamine administration on intestinal adaptation in a rat model of SBS. Fourteen male Wistar rats were divided into two groups; all 14 underwent 75% small bowel resection. Within each group, rats were assigned to 14 days of treatment with subcutaneous glutamine (0.3 g/kg/day) or isotonic saline daily. Weight changes and histological intestinal adaptation parameters (mucosal thickness, villus height, and crypt depth) were measured. Non-mucosal intestinal changes were evaluated by intestinal fractioned collagen analysis. All rats initially lost weight and began to gain weight postoperatively; however, they did not reach their preoperative weights during the experiment and there was no significant difference between the groups. Histological adaptation parameters were significantly increased after 75% intestinal resection in both groups compared to paired native samples (P<0.01); although the percent of increase was slightly higher in Gln group, no significant difference was detected between the two groups. Fractioned-collagen amounts were found to be similar between groups. The results indicated that parenteral glutamine administration alone does not improve the intestinal adaptation process after massive intestinal resection in rats.

show abstract

Adaptation following intestinal resection: mechanisms and signals

Cited by 39 publications

References 102 publications

Maternal protein restriction during pregnancy affects gene expression and immunolocalization of intestinal nutrient transporters in rats

Maternal protein restriction during pregnancy affects gene expression and immunolocalization of intestinal nutrient transporters in rats

Impact of Ileocecal Resection and Concomitant Antibiotics on the Microbiome of the Murine Jejunum and Colon

The effects of parenteral glutamine on intestinal adaptation in a rat model of short bowel syndrome

Contact Info

Product

Resources

About