Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic inhibitors by cell recycle batch fermentation

Landaeta, Roberto; Aroca, Germán; Acevedo, Fernando; Teixeira, J. A.; Mussatto, Solange I.

doi:10.1016/j.apenergy.2012.06.048

Cited by 53 publications

(38 citation statements)

References 38 publications

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“…Biotechnol. Biofuels 9, 167. doi:10.1186/s13068-016-0570-6 vanillin, syringaldehyde, and hydroxybenzoic acid have also been developed in ethanologenic yeast by approaching adaptation strategy (Landaeta et al, 2013;Slininger et al, 2015). In another study, adaptation of S. stipitis was performed in acid hydrolyzate and evolved strain showed enhanced tolerance with improved fermentation of hydrolyzate in shorter duration (Nigam, 2001).…”

Section: −1mentioning

confidence: 99%

“…Both the strains were cultivated in 500-ml cotton plugged Erlenmeyer flasks with working volume of 100 ml in YEP medium containing 20 g l −1 xylose for 24 h. Figure 2 shows the growth pattern of K. marxianus NIRE-K3 and Landaeta et al (2013), and Jõers and Tenson (2016) also reported the shorter lag phase for adapted strains. Moreover, xylose utilization of K. marxianus NIRE-K3.1 was about 3.21-fold higher as compare to K. marxianus NIRE-K3 (Figure 2).…”

Section: Adaptation Of K Marxianus Nire-k3mentioning

confidence: 99%

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Evolutionary Adaptation of Kluyveromyces marxianus NIRE-K3 for Enhanced Xylose Utilization

et al. 2017

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The evolutionary adaptation was approached on the thermotolerant yeast Kluyveromyces marxianus NIRE-K3 at 45°C on xylose as a sole source of carbon for enhancement of xylose uptake. After 60 cycles, evolved strain K. marxianus NIRE-K3.1 showed comparatively 3.75-and 3.0-fold higher specific growth and xylose uptake rates, respectively, than that of native strain. Moreover, the short lag phase was also observed on adapted strain. During batch fermentation with xylose concentration of 30 g l −1, K. marxianus NIRE-K3.1 could utilize about 96% of xylose in 72 h and produced 4.67 and 15.7 g l −1 of ethanol and xylitol, respectively, which were 9.72-and 4.63-fold higher than that of native strain. Similarly, specific sugar consumption rate, xylitol, and ethanol yields were 5.07-, 1.15-, and 2.44-fold higher as compared to the native strain, respectively. The results obtained after evolutionary adaptation of K. marxianus NIRE-K3 show the significant improvement in the xylose utilization, ethanol and xylitol yields, and productivities. By understanding the results obtained, the significance of evolutionary adaptation has been rationalized, since the adapted culture could be more stable and could enhance the productivity.

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Section: −1mentioning

confidence: 99%

Section: Adaptation Of K Marxianus Nire-k3mentioning

confidence: 99%

Evolutionary Adaptation of Kluyveromyces marxianus NIRE-K3 for Enhanced Xylose Utilization

et al. 2017

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“…As leveduras 56 algumas leveduras apresentam a capacidade de converter compostos tóxicos, gerados na etapa de hidrólise ácida de materiais lignocelulósicos, em compostos com menor toxicidade, porém com reflexo sobre a fase de latência, que se torna mais longa, e com consequente redução da produtividade. Não foram observados efeitos dos inibidores presentes no hidrolisado ácido da torta de girassol que tivessem prolongado a fase lag de crescimento (Figura 2).…”

Section: Fermentação Alcoólica Do Hidrolisado Hemicelulósicounclassified

FERMENTAÇÃO ALCOÓLICA DE HIDROLISADO HEMICELULÓSICO DE TORTA DE GIRASSOL POR Galactomyces geotrichum UFVJM-R10 E Candida akabanensis UFVJM-R131

Matos¹,

Souza²,

Santos³

et al. 2017

Quim. Nova

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ALCOHOLIC FERMENTATION OF HEMICELLULOSIC HYDROLYZATE FROM SUNFLOWER CAKE BYGalactomyces geotrichum UFVJM-R10 AND Candida akabanensis UFVJM-R131. The use of the hemicellulosic fraction of plants for the production of second generation bioethanol depends on microorganisms capable to ferment pentoses. Two yeast strains habile to xylose fermenting in synthetic medium, Candida akabanensis UFVJM-R131 and Galactomyces geotrichum UFVJM-R10, not yet registered in the literature for the production of bioethanol, were evaluated here in the alcoholic fermentation of the hemicellulosic hydrolyzate from sunflower cake. The biomass hydrolysis was performed by 38 minutes at 120 °C / 1 atm with 6% H 2 SO 4 solution and solid / liquid ratio of 1:3.2. Chromatographic characterization of the hemicellulosic hydrolyzate showed the presence of glucose (7.57 g L ). Both yeasts were able to produce ethanol from the acid hydrolyzate from sunflower cake. The fermentation carried out with G. geotrichum UFVJM-R10 and C. akabanensis UFVJM-R131 presented Y P/S values of 0.29 and 0.27 g ethanol g -1 sugars , respectively. The amounts of the inhibitors identified in the hydrolyzate did not affect the efficiency of the alcoholic fermentation. The supplementation of the hydrolyzate with nitrogen and mineral sources increased the rate of consumption of xylose and arabinose.

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“…In these processes, high yields can be reached because it is possible to operate with a high concentration of cells (e.g., 45% v /v) inside the reactor [5,7,8]. Moreover, using a high cell density, it is possible to achieve a high ethanol concentration in the fermented juice, a goal of the industry [9]. The cell-recycling approach retains the flocculent yeast in the fermentor and provides a more convenient way to maintain high cell concentration in the reactor.…”

Section: Introductionmentioning

confidence: 98%

Continuous ethanol fermentation in tower reactors with cell recycling using flocculent Saccharomyces cerevisiae

Santos

Sousa

Guidini

et al. 2015

Process Biochemistry

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Adaptation of a flocculent Saccharomyces cerevisiae strain to lignocellulosic inhibitors by cell recycle batch fermentation

Cited by 53 publications

References 38 publications

Evolutionary Adaptation of Kluyveromyces marxianus NIRE-K3 for Enhanced Xylose Utilization

Evolutionary Adaptation of Kluyveromyces marxianus NIRE-K3 for Enhanced Xylose Utilization

FERMENTAÇÃO ALCOÓLICA DE HIDROLISADO HEMICELULÓSICO DE TORTA DE GIRASSOL POR Galactomyces geotrichum UFVJM-R10 E Candida akabanensis UFVJM-R131

Continuous ethanol fermentation in tower reactors with cell recycling using flocculent Saccharomyces cerevisiae

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