Adaptation of Candida shehatae and Pichia stipitis to wood hydrolysates for increased ethanol production

Parekh, Sarad R.; Yu, Shiyuan; Wayman, Morris

doi:10.1007/bf00253667

Cited by 92 publications

(42 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The fermentative production of ethanol was markedly improved by using cells previously adapted to the culture medium (CHEN and GONG 1985, PAREKH et al 1986, YU et al 1987. Thus, this approach could become an alternative to the adsorption and/or precipitation of the sugar cane bagasse hydrolysate.…”

mentioning

confidence: 98%

Adaptation and reutilization ofCandida guilliermondii cells for xylitol production in bagasse hydrolysate

Sene¹,

Felipe²,

Vítolo

et al. 1998

J. Basic Microbiol.

View full text Add to dashboard Cite

mentioning

confidence: 98%

Adaptation and reutilization ofCandida guilliermondii cells for xylitol production in bagasse hydrolysate

Sene¹,

Felipe²,

Vítolo

et al. 1998

J. Basic Microbiol.

View full text Add to dashboard Cite

“…Besides the carbon source and aeration, yeast requires substances for the cellular synthesis and energy, such as nitrogen source. In the present work, nitrogen source was added in the synthetic medium as proposed by PAREKH et al (1986).…”

Section: Resultsmentioning

confidence: 99%

“…The fermentation assays were carried out in 125 ml Erlenmeyer flask containing 50 ml of synthetic xylose solution (37 g/L) (supplemented with Media defined by Parekh et al, (1986) (5g/L of ammonium sulfate, 3g/L of yeast extract and 3g/L of malt extract) and initial cell concentration (0.5 g/l) of S. shehatae UFMG 52.2. Flasks were incubated in a rotator shaker (Innova 4000 Incubator Shaker, New Brunswick Scientific, Enfield, CT, USA) at 200 rpm and 30 ºC for 72 h.…”

Section: Fermentationmentioning

confidence: 99%

Avaliação de um nova levedura da biodiversidade brasileira, Scheffersomyces shehatae UMFG-HM 52.2, para a conversão de pentoses em bioetanol

Antunes¹,

Milessi²,

Chandel³

et al. 2013

BBR - Biochem. Biotechnol.

View full text Add to dashboard Cite

Hemicellulose, a heterogeneous polymer of various sugars linked with xylose backbone, is the second most available carbohydrate polymer on Earth, after cellulose. Hemicellulose can be hydrolysed by thermochemical or enzyme mediated action into a mixed sugar solution primarily composed by xylose. Bioconversion of hemicellulose derived sugars into ethanol is an important criterion to get the satisfactory overall bioethanol yield from lignocellulosic materials. However, the development of hemicellulose conversion into ethanol requires additional challenges. Only few microorganisms are able to ferment pentose sugars into ethanol. Xylose-utilizing microorganisms have shown low rates of sugar assimilation, conversion and poor tolerance to ethanol. These factors often prevent their use in technological processes at large scale. In this line, the search for new microorganisms that efficiently assimilate pentoses, particularly xylose, is a priority within the context of the integral use of lignocellulosic biomass into ethanol. This study showed the improved fermentation of synthetic xylose into ethanol under batch cultivations by a new strain of Scheffersomyces shehatae (UMFG-HM 52.2), isolated from Brazilian Atlantic rain forest ecosystem. Ethanol production performance of this yeast was evaluated in synthetic medium (using supplemented synthetic xylose as carbon source) in 125mL Erlenmeyer flaks at 200 rpm contained 50 mL of medium incubated for 72 h at 30°C. After 18 h of incubation, ethanol yield (Y P/S ) of 0.39 g/g and productivity (Qp) of 0.79 g/L was obtained. This study showed the potential of S. shehatae UMFG-HM 52.2 as a promising candidate in process to produce ethanol from pentose sugars. RESUMOA hemicelulose, polímero heterogêneo composto por diferentes monossacarídeos ligados em uma estrutura principal de xilose, depois da celulose, é o segundo carboidrato polimérico mais abundante na terra. A hemicelulose pode ser hidrolisada por meio termoquímico ou enzimático, em uma solução mista de açúcares, com a predominância de xilose. A bioconversão de açúcares derivados de hemicelulose em etanol é um critério importante para obter-se rendimentos satisfatórios na produção de bioetanol a partir de materiais lignocelulósicos. Entretanto, o desenvolvimento da conversão da hemicelulose em etanol requer desafios adicionais. Os microrganismos para a conversão de pentoses em etanol são poucos. Microrganismos fermentadores de xilose têm demonstrado baixos índices de assimilação e conversão de açúcares e baixa tolerância ao etanol. Esses fatores são importantes pois muitas vezes impedem a sua utilização em processos tecnológicos em larga escala. Neste contexto, a busca de novos microrganismos que assimilam eficientemente pentoses, particularmente a xilose, é uma prioridade dentro do contexto da utilização integral da biomassa lignocelulósica em etanol. Este estudo apresentou a fermentação de xilose em etanol sob cultivo em batelada por uma nova linhagem de levedura, Scheffersomyces shehatae (UMFG-HM 52.2), isolada...

show abstract

“…However, its ethanol tolerance was lower than that of even E. coli. Scheffersomyces stipitis (Pichia stipitis) is one of the naturally xylose fermenting strains, which can also ferment galactose, glucose, and cellobiose (Parekh et al 1986). The redox balance between xylose reductase and xylitol dehydrogenase is less drastic in this yeast because the respective xylose reductase can use both NADH and NADPH (Verduyn et al 1985).…”

Section: Consumption Of Multiple Sugarsmentioning

confidence: 99%

Can Microbially Derived Advanced Biofuels Ever Compete with Conventional Bioethanol? A Critical Review

Veettil

Kumar

Koukoulas³

2016

BioResources

View full text Add to dashboard Cite

Adaptation of Candida shehatae and Pichia stipitis to wood hydrolysates for increased ethanol production

Cited by 92 publications

References 7 publications

Adaptation and reutilization ofCandida guilliermondii cells for xylitol production in bagasse hydrolysate

Adaptation and reutilization ofCandida guilliermondii cells for xylitol production in bagasse hydrolysate

Avaliação de um nova levedura da biodiversidade brasileira, Scheffersomyces shehatae UMFG-HM 52.2, para a conversão de pentoses em bioetanol

Can Microbially Derived Advanced Biofuels Ever Compete with Conventional Bioethanol? A Critical Review

Contact Info

Product

Resources

About