Adaptation of Carbon Source Utilization Patterns of Geobacter metallireducens During Sessile Growth

Marozava, Sviatlana; Merl-Pham, Juliane; Müller, Hubert

doi:10.3389/fmicb.2020.01271

Cited by 3 publications

(1 citation statement)

References 66 publications

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“…Methane oxidation was detected on the oxygen-rich site while methanol catabolism was observed on all polluted sites ( Bargiela et al, 2015 ). In a recent study, Marozava and colleagues used metagenomics and metaproteomics to establish a specific expression profile of catabolic pathways for aromatics biodegradation by Geobacter metallireducens during sessile growth ( Marozava et al, 2020 ).…”

Section: Multi-omics Techniques Applied In Anaerobic Hydrocarbon Biodmentioning

confidence: 99%

New Frontiers of Anaerobic Hydrocarbon Biodegradation in the Multi-Omics Era

et al. 2020

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The accumulation of petroleum hydrocarbons in the environment substantially endangers terrestrial and aquatic ecosystems. Many microbial strains have been recognized to utilize aliphatic and aromatic hydrocarbons under aerobic conditions. Nevertheless, most of these pollutants are transferred by natural processes, including rain, into the underground anaerobic zones where their degradation is much more problematic. In oxic zones, anaerobic microenvironments can be formed as a consequence of the intensive respiratory activities of (facultative) aerobic microbes. Even though aerobic bioremediation has been well-characterized over the past few decades, ample research is yet to be done in the field of anaerobic hydrocarbon biodegradation. With the emergence of high-throughput techniques, known as omics (e.g., genomics and metagenomics), the individual biodegraders, hydrocarbon-degrading microbial communities and metabolic pathways, interactions can be described at a contaminated site. Omics approaches provide the opportunity to examine single microorganisms or microbial communities at the system level and elucidate the metabolic networks, interspecies interactions during hydrocarbon mineralization. Metatranscriptomics and metaproteomics, for example, can shed light on the active genes and proteins and functional importance of the less abundant species. Moreover, novel unculturable hydrocarbon-degrading strains and enzymes can be discovered and fit into the metabolic networks of the community. Our objective is to review the anaerobic hydrocarbon biodegradation processes, the most important hydrocarbon degraders and their diverse metabolic pathways, including the use of various terminal electron acceptors and various electron transfer processes. The review primarily focuses on the achievements obtained by the current high-throughput (multi-omics) techniques which opened new perspectives in understanding the processes at the system level including the metabolic routes of individual strains, metabolic/electric interaction of the members of microbial communities. Based on the multi-omics techniques, novel metabolic blocks can be designed and used for the construction of microbial strains/consortia for efficient removal of hydrocarbons in anaerobic zones.

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Section: Multi-omics Techniques Applied In Anaerobic Hydrocarbon Biodmentioning

confidence: 99%

New Frontiers of Anaerobic Hydrocarbon Biodegradation in the Multi-Omics Era

et al. 2020

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Validation of 16S rRNA gene sequencing and metagenomics for evaluating microbial immigration in a methanogenic bioreactor

Lin,

Liu

2023

Water Research

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Buried wood effects on macronutrient supply and microbial activity and metabolic potential in different oil sands reclamation soils in northern Alberta

et al. 2023

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Buried wood is an important yet understudied component of natural and anthropogenic soils. Nutrient immobilization as a response to wood addition during oil sands' reclamation may be a concern since surface wood is salvaged with the soil, thereby becoming buried wood in reclamation cover soils. This project investigated the impact of buried wood on macronutrient supply and microbial communities in different reclamation soils. A 60-day incubation was performed with different rates and types of wood (0%–50%, aspen and pine) and four different soils: fine and coarse forest floor-mineral mix (fFFMM and cFFMM), peat-mineral mix (PMM), and peat. Analysis of macronutrient supply rates using Plant Root Simulator (PRS™) probes and a community-level physiological profiling (CLPP) to assess metabolic potential was performed at the end of the incubation period; microbial activity was measured through soil respiration during the incubation. Responses varied by soil type; however, buried wood caused nitrogen immobilization in three soils due to an increase in the C:N ratio. Soils with lower C:N ratios like fFFMM and PMM were more susceptible to immobilization with a decrease in available nitrogen by up to 95% at a 10% of wood addition. Phosphorus immobilization was observed in cFFMM, and potassium supply increased at 20% of wood and above. Soil microbial activity and metabolic potential increased but no significant changes in the soil profiles were observed. The findings of this study demonstrate that buried wood increases the soil C:N ratio and can potentially cause nitrogen immobilization when added by 10% of volume or more.

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Adaptation of Carbon Source Utilization Patterns of Geobacter metallireducens During Sessile Growth

Cited by 3 publications

References 66 publications

New Frontiers of Anaerobic Hydrocarbon Biodegradation in the Multi-Omics Era

New Frontiers of Anaerobic Hydrocarbon Biodegradation in the Multi-Omics Era

Validation of 16S rRNA gene sequencing and metagenomics for evaluating microbial immigration in a methanogenic bioreactor

Buried wood effects on macronutrient supply and microbial activity and metabolic potential in different oil sands reclamation soils in northern Alberta

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